ENSG00000067082

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000497571 ENSG00000067082 HEK293_OSMI2_2hA HEK293_TMG_2hB KLF6 protein_coding protein_coding 5.664217 2.537555 7.343875 0.467086 0.3498031 1.529391 4.736626 2.421238 5.95081 0.4271411 0.1995663 1.293817 0.8615042 0.9565667 0.8136667 -0.1429 0.01810127 0.01810127 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000067082 E001 0.0000000       10 3775996 3775996 1 -      
ENSG00000067082 E002 0.2955422 0.0277940714 5.132849e-01   10 3775997 3776403 407 - 0.075 0.194 1.579
ENSG00000067082 E003 315.2363001 0.0066423686 3.916754e-11 7.398716e-10 10 3776404 3779046 2643 - 2.371 2.624 0.845
ENSG00000067082 E004 48.0835168 0.0005279270 3.849704e-04 1.716721e-03 10 3779047 3779346 300 - 1.680 1.569 -0.378
ENSG00000067082 E005 39.0946033 0.0006033336 1.113954e-02 3.150061e-02 10 3779347 3779549 203 - 1.580 1.512 -0.233
ENSG00000067082 E006 18.2381124 0.0010069149 5.053544e-02 1.101106e-01 10 3779550 3779590 41 - 1.267 1.178 -0.313
ENSG00000067082 E007 38.0812890 0.0024473741 2.898541e-02 6.997833e-02 10 3780106 3780229 124 - 1.567 1.512 -0.189
ENSG00000067082 E008 2.5214689 0.0075286645 2.745707e-01 4.137463e-01 10 3780230 3780326 97 - 0.560 0.429 -0.642
ENSG00000067082 E009 9.0696131 0.0020102908 3.513621e-01 4.955812e-01 10 3780327 3781640 1314 - 0.976 0.948 -0.103
ENSG00000067082 E010 40.8893162 0.0042967544 1.856241e-02 4.836797e-02 10 3781641 3781766 126 - 1.601 1.533 -0.233
ENSG00000067082 E011 27.0666107 0.0007581264 7.860256e-02 1.572591e-01 10 3781767 3781794 28 - 1.421 1.382 -0.138
ENSG00000067082 E012 25.2387306 0.0007404063 3.981802e-03 1.309164e-02 10 3781795 3781807 13 - 1.415 1.279 -0.473
ENSG00000067082 E013 80.0834084 0.0003573694 3.442400e-07 3.146542e-06 10 3781808 3782214 407 - 1.902 1.771 -0.442
ENSG00000067082 E014 1.0298247 0.0124257245 3.635848e-01 5.079987e-01 10 3783216 3783281 66 - 0.328 0.194 -1.004
ENSG00000067082 E015 31.9797805 0.0144922942 4.015680e-01 5.452838e-01 10 3784913 3785281 369 - 1.476 1.474 -0.006