ENSG00000066827

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000377838 ENSG00000066827 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAT protein_coding protein_coding 4.147788 6.699668 2.425411 0.4090942 0.09692878 -1.462076 3.1683586 4.3183830 2.317755 0.7015203 0.05495912 -0.894889 0.7901042 0.6528000 0.9572 0.3044000 4.712518e-02 4.087885e-06 FALSE TRUE
ENST00000517307 ENSG00000066827 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAT protein_coding processed_transcript 4.147788 6.699668 2.425411 0.4090942 0.09692878 -1.462076 0.2087333 0.4094686 0.000000 0.4094686 0.00000000 -5.390491 0.0407875 0.0548000 0.0000 -0.0548000 9.420104e-01 4.087885e-06 FALSE FALSE
ENST00000518408 ENSG00000066827 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFAT protein_coding retained_intron 4.147788 6.699668 2.425411 0.4090942 0.09692878 -1.462076 0.4911497 1.7943717 0.000000 0.5469008 0.00000000 -7.495353 0.0779375 0.2652667 0.0000 -0.2652667 4.087885e-06 4.087885e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000066827 E001 0.1472490 0.0423655761 1.842056e-01   8 134477788 134477791 4 - 0.183 0.000 -10.936
ENSG00000066827 E002 21.9971582 0.0008694557 6.234512e-03 0.0192291083 8 134477792 134478024 233 - 1.099 1.346 0.872
ENSG00000066827 E003 49.8163012 0.0007252466 2.461771e-03 0.0086490702 8 134478025 134478221 197 - 1.498 1.679 0.617
ENSG00000066827 E004 49.7756764 0.0013598618 2.293617e-03 0.0081305116 8 134478222 134478392 171 - 1.491 1.677 0.636
ENSG00000066827 E005 72.8473204 0.0047216505 2.203488e-03 0.0078534876 8 134478393 134478701 309 - 1.660 1.836 0.598
ENSG00000066827 E006 28.9072678 0.0224068765 4.895506e-01 0.6269606580 8 134478702 134478721 20 - 1.353 1.422 0.237
ENSG00000066827 E007 40.4539724 0.0100313775 5.276815e-02 0.1140792932 8 134509619 134509700 82 - 1.427 1.582 0.530
ENSG00000066827 E008 24.6325735 0.0010026609 4.146102e-02 0.0937389162 8 134509701 134509708 8 - 1.211 1.380 0.592
ENSG00000066827 E009 34.6525279 0.0006562985 7.934665e-03 0.0236136609 8 134509709 134509749 41 - 1.332 1.522 0.655
ENSG00000066827 E010 0.0000000       8 134510062 134510182 121 -      
ENSG00000066827 E011 0.0000000       8 134510696 134511082 387 -      
ENSG00000066827 E012 45.4347345 0.0005554288 8.713002e-02 0.1707937075 8 134512475 134512573 99 - 1.519 1.622 0.353
ENSG00000066827 E013 30.4884114 0.0007118733 5.460881e-01 0.6763311398 8 134512574 134512601 28 - 1.401 1.444 0.149
ENSG00000066827 E014 45.3927534 0.0005591910 2.521934e-01 0.3885152523 8 134520883 134521001 119 - 1.546 1.614 0.232
ENSG00000066827 E015 42.5744792 0.0005781609 3.496189e-01 0.4938285476 8 134532834 134532972 139 - 1.526 1.583 0.195
ENSG00000066827 E016 0.5180316 0.0246219125 3.613707e-01 0.5057594141 8 134564987 134565152 166 - 0.000 0.205 12.606
ENSG00000066827 E017 32.0927420 0.0006774586 3.353506e-01 0.4791297637 8 134565333 134565421 89 - 1.401 1.469 0.234
ENSG00000066827 E018 0.3299976 0.0274424043 6.150433e-01   8 134565422 134565671 250 - 0.000 0.147 12.033
ENSG00000066827 E019 43.7514497 0.0006644690 4.409719e-01 0.5826206439 8 134583832 134583950 119 - 1.546 1.592 0.158
ENSG00000066827 E020 29.1534911 0.0171874765 6.568307e-01 0.7662965929 8 134583951 134584005 55 - 1.382 1.419 0.126
ENSG00000066827 E021 43.8441522 0.0005796080 9.440302e-01 0.9689328884 8 134588246 134588395 150 - 1.589 1.585 -0.013
ENSG00000066827 E022 31.5836507 0.0023170883 8.840863e-01 0.9298946722 8 134590268 134590355 88 - 1.444 1.456 0.041
ENSG00000066827 E023 0.0000000       8 134594807 134594885 79 -      
ENSG00000066827 E024 0.0000000       8 134597544 134599729 2186 -      
ENSG00000066827 E025 0.2214452 0.0404776728 1.000000e+00   8 134599730 134599862 133 - 0.000 0.080 10.999
ENSG00000066827 E026 0.1451727 0.0428985887 1.838817e-01   8 134599863 134600435 573 - 0.183 0.000 -13.661
ENSG00000066827 E027 51.0055902 0.0049133701 1.611207e-01 0.2765766076 8 134600436 134600668 233 - 1.716 1.633 -0.281
ENSG00000066827 E028 123.5628066 0.0003440411 2.634165e-04 0.0012305741 8 134601477 134602806 1330 - 2.118 1.999 -0.400
ENSG00000066827 E029 22.1948608 0.0113908271 5.270461e-02 0.1139666269 8 134602807 134602933 127 - 1.427 1.253 -0.606
ENSG00000066827 E030 12.8936910 0.0016231716 1.394800e-03 0.0052898903 8 134608729 134608739 11 - 1.288 0.985 -1.085
ENSG00000066827 E031 22.5646934 0.0008708315 1.643878e-04 0.0008109044 8 134608740 134608840 101 - 1.505 1.228 -0.962
ENSG00000066827 E032 13.1794662 0.0014235969 1.232662e-02 0.0343170584 8 134608841 134608879 39 - 1.263 1.028 -0.843
ENSG00000066827 E033 30.8394206 0.0007150005 5.174318e-06 0.0000366987 8 134610470 134610655 186 - 1.639 1.350 -0.994
ENSG00000066827 E034 39.6389916 0.0037225734 6.439385e-03 0.0197647871 8 134637461 134637712 252 - 1.669 1.499 -0.580
ENSG00000066827 E035 0.2214452 0.0404776728 1.000000e+00   8 134639775 134639880 106 - 0.000 0.080 10.999
ENSG00000066827 E036 27.1531973 0.0100006578 2.454619e-02 0.0609423035 8 134657561 134657737 177 - 1.512 1.331 -0.626
ENSG00000066827 E037 0.4815130 0.0217653909 8.212973e-01 0.8873353591 8 134674730 134674888 159 - 0.183 0.147 -0.384
ENSG00000066827 E038 0.0000000       8 134696431 134696558 128 -      
ENSG00000066827 E039 15.9356692 0.0242235047 4.157399e-01 0.5590987690 8 134712845 134713049 205 - 1.224 1.136 -0.314