ENSG00000066777

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262215 ENSG00000066777 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF1 protein_coding protein_coding 6.68801 3.355437 9.384141 0.2211302 0.4314122 1.480968 3.4235900 1.38035013 4.9068605 0.17056066 0.07758611 1.822289 0.47421667 0.421300000 0.5243333 0.10303333 4.793708e-01 1.551894e-18 FALSE TRUE
ENST00000517955 ENSG00000066777 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF1 protein_coding retained_intron 6.68801 3.355437 9.384141 0.2211302 0.4314122 1.480968 0.4936228 0.95464737 0.0000000 0.32200977 0.00000000 -6.591930 0.12931667 0.274666667 0.0000000 -0.27466667 6.143470e-11 1.551894e-18 FALSE FALSE
ENST00000522878 ENSG00000066777 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF1 protein_coding processed_transcript 6.68801 3.355437 9.384141 0.2211302 0.4314122 1.480968 0.3872671 0.42449038 0.0000000 0.21294171 0.00000000 -5.441252 0.08905417 0.125300000 0.0000000 -0.12530000 4.997644e-02 1.551894e-18 FALSE FALSE
MSTRG.31630.5 ENSG00000066777 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF1 protein_coding   6.68801 3.355437 9.384141 0.2211302 0.4314122 1.480968 1.1575006 0.00000000 2.8640758 0.00000000 0.30981960 8.166954 0.12106250 0.000000000 0.3039000 0.30390000 1.551894e-18 1.551894e-18 FALSE TRUE
MSTRG.31630.6 ENSG00000066777 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF1 protein_coding   6.68801 3.355437 9.384141 0.2211302 0.4314122 1.480968 0.4830257 0.03525253 0.4226429 0.02451193 0.22906026 3.257106 0.05535417 0.009666667 0.0460000 0.03633333 7.476636e-01 1.551894e-18 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000066777 E001 10.9830773 0.0024722891 2.576427e-03 8.993686e-03 8 67173511 67175486 1976 - 0.905 1.237 1.211
ENSG00000066777 E002 2.1036552 0.0069012008 7.213181e-01 8.157423e-01 8 67175487 67175572 86 - 0.409 0.482 0.382
ENSG00000066777 E003 1.2596890 0.0106239456 6.832311e-01 7.865012e-01 8 67197658 67197673 16 - 0.287 0.372 0.533
ENSG00000066777 E004 89.3697873 0.0004641419 1.706069e-37 5.011003e-35 8 67197674 67198404 731 - 1.702 2.209 1.703
ENSG00000066777 E005 123.6963401 0.0059327813 1.457406e-12 3.476032e-11 8 67198405 67199010 606 - 1.912 2.260 1.167
ENSG00000066777 E006 46.1647354 0.0004687957 1.702276e-04 8.359888e-04 8 67199011 67199098 88 - 1.533 1.758 0.765
ENSG00000066777 E007 2.8271502 0.0122537415 4.223377e-03 1.376715e-02 8 67199099 67199928 830 - 0.381 0.851 2.115
ENSG00000066777 E008 2.6312543 0.0066447815 4.204177e-01 5.636203e-01 8 67199929 67200395 467 - 0.524 0.372 -0.788
ENSG00000066777 E009 46.9046592 0.0034683716 2.460176e-03 8.644588e-03 8 67200396 67200513 118 - 1.549 1.747 0.675
ENSG00000066777 E010 52.5373423 0.0055586334 4.940998e-02 1.081085e-01 8 67201467 67201605 139 - 1.612 1.749 0.463
ENSG00000066777 E011 0.2965864 0.2779693274 3.566881e-02   8 67201606 67201889 284 - 0.000 0.365 12.016
ENSG00000066777 E012 63.3268734 0.0004444407 6.759400e-04 2.811055e-03 8 67203083 67203251 169 - 1.679 1.859 0.608
ENSG00000066777 E013 39.6097136 0.0005372900 7.324937e-03 2.206426e-02 8 67204680 67204766 87 - 1.482 1.660 0.608
ENSG00000066777 E014 30.5103012 0.0039821746 2.081868e-02 5.320912e-02 8 67204767 67204819 53 - 1.374 1.559 0.636
ENSG00000066777 E015 0.8889184 0.0135404003 8.960378e-01 9.375592e-01 8 67207095 67207190 96 - 0.251 0.225 -0.205
ENSG00000066777 E016 40.6331920 0.0045111780 1.039540e-02 2.973453e-02 8 67211483 67211615 133 - 1.493 1.679 0.632
ENSG00000066777 E017 20.8737600 0.0008533512 7.870145e-01 8.636257e-01 8 67216590 67216607 18 - 1.252 1.286 0.118
ENSG00000066777 E018 33.4078387 0.0006343737 3.778989e-02 8.694448e-02 8 67216608 67216662 55 - 1.415 1.568 0.524
ENSG00000066777 E019 47.4216902 0.0009346332 7.712267e-02 1.548740e-01 8 67217782 67217920 139 - 1.576 1.691 0.391
ENSG00000066777 E020 45.4818972 0.0005245840 2.059100e-01 3.335604e-01 8 67218003 67218138 136 - 1.567 1.654 0.294
ENSG00000066777 E021 42.8117990 0.0005089797 4.361595e-01 5.782789e-01 8 67219431 67219560 130 - 1.547 1.606 0.201
ENSG00000066777 E022 39.4917156 0.0024699263 1.119133e-01 2.083776e-01 8 67224903 67225033 131 - 1.496 1.613 0.401
ENSG00000066777 E023 45.3614613 0.0046531852 6.102814e-01 7.296203e-01 8 67226023 67226183 161 - 1.587 1.560 -0.092
ENSG00000066777 E024 44.2144518 0.0004851206 4.538351e-01 5.945446e-01 8 67227137 67227309 173 - 1.582 1.543 -0.133
ENSG00000066777 E025 46.9791434 0.0006054350 7.505009e-01 8.371762e-01 8 67227447 67227598 152 - 1.591 1.620 0.100
ENSG00000066777 E026 41.4495999 0.0005156784 6.808681e-01 7.846965e-01 8 67227963 67228132 170 - 1.539 1.575 0.124
ENSG00000066777 E027 19.6497755 0.0009847084 5.851481e-01 7.089020e-01 8 67228224 67228264 41 - 1.244 1.202 -0.151
ENSG00000066777 E028 37.8991200 0.0005952787 3.715728e-02 8.577547e-02 8 67232855 67232945 91 - 1.537 1.393 -0.494
ENSG00000066777 E029 45.8507702 0.0013761087 3.548822e-03 1.186513e-02 8 67238343 67238493 151 - 1.624 1.428 -0.672
ENSG00000066777 E030 39.8116360 0.0193632855 2.609049e-02 6.412930e-02 8 67238735 67238893 159 - 1.565 1.359 -0.708
ENSG00000066777 E031 26.4725464 0.0007710151 7.547029e-01 8.402643e-01 8 67240162 67240290 129 - 1.359 1.343 -0.057
ENSG00000066777 E032 36.4325232 0.0025697549 9.712809e-01 9.860682e-01 8 67251299 67251450 152 - 1.487 1.499 0.042
ENSG00000066777 E033 43.2566080 0.0198756378 5.038581e-02 1.098503e-01 8 67253451 67253622 172 - 1.596 1.420 -0.604
ENSG00000066777 E034 31.3143566 0.0068249433 1.291119e-01 2.329386e-01 8 67257732 67257816 85 - 1.453 1.329 -0.426
ENSG00000066777 E035 44.6196118 0.0014714119 1.936595e-02 5.012735e-02 8 67258085 67258290 206 - 1.604 1.449 -0.530
ENSG00000066777 E036 33.3029157 0.0018205688 2.796714e-02 6.794814e-02 8 67259815 67259926 112 - 1.485 1.315 -0.586
ENSG00000066777 E037 47.9349859 0.0005616252 4.305154e-03 1.399809e-02 8 67266006 67266207 202 - 1.641 1.459 -0.622
ENSG00000066777 E038 36.4538056 0.0006629633 4.771160e-05 2.701865e-04 8 67266876 67266984 109 - 1.545 1.220 -1.127
ENSG00000066777 E039 33.6966724 0.0006010905 3.546425e-04 1.598068e-03 8 67267091 67267230 140 - 1.508 1.220 -1.002
ENSG00000066777 E040 21.2354043 0.0008667299 1.485758e-02 4.016844e-02 8 67267343 67267442 100 - 1.309 1.074 -0.836
ENSG00000066777 E041 40.9485530 0.0005465073 2.654523e-06 2.011003e-05 8 67271702 67271936 235 - 1.599 1.237 -1.252
ENSG00000066777 E042 39.2399324 0.0009400026 8.760594e-08 8.992080e-07 8 67275976 67276109 134 - 1.589 1.142 -1.553
ENSG00000066777 E043 46.1489747 0.0004837220 7.011071e-11 1.269572e-09 8 67277282 67277457 176 - 1.664 1.142 -1.809
ENSG00000066777 E044 34.4410479 0.0006024872 1.381015e-05 8.880795e-05 8 67287955 67288065 111 - 1.527 1.163 -1.268
ENSG00000066777 E045 51.3162038 0.0004483182 2.135606e-08 2.476641e-07 8 67291847 67292123 277 - 1.697 1.301 -1.360
ENSG00000066777 E046 29.6462129 0.0006682797 4.078128e-08 4.478518e-07 8 67296431 67296610 180 - 1.478 0.927 -1.962
ENSG00000066777 E047 20.9312306 0.0249536152 1.259835e-03 4.842788e-03 8 67299209 67299313 105 - 1.326 0.892 -1.569
ENSG00000066777 E048 13.4984663 0.0148809481 1.639616e-02 4.360686e-02 8 67299314 67299355 42 - 1.135 0.809 -1.219
ENSG00000066777 E049 21.8861431 0.0009269899 7.717611e-06 5.261356e-05 8 67301224 67301380 157 - 1.350 0.851 -1.810
ENSG00000066777 E050 13.2968047 0.0013193227 2.443919e-04 1.151753e-03 8 67302436 67302466 31 - 1.150 0.642 -1.953
ENSG00000066777 E051 18.6722339 0.0010416986 6.019906e-05 3.324161e-04 8 67343164 67343336 173 - 1.281 0.808 -1.740
ENSG00000066777 E052 8.0314391 0.0173043037 1.712959e-04 8.406472e-04 8 67343337 67343509 173 - 0.968 0.225 -3.610
ENSG00000066777 E053 13.0553403 0.0105185167 3.248501e-05 1.916118e-04 8 67343510 67343787 278 - 1.150 0.482 -2.689