ENSG00000066735

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000315264 ENSG00000066735 HEK293_OSMI2_2hA HEK293_TMG_2hB KIF26A protein_coding protein_coding 10.03922 14.90609 6.570928 1.710019 0.1377457 -1.180506 8.1560970 13.8226668 2.7158178 1.3226933 1.3581269 -2.343318 0.77817083 0.93330000 0.4154000 -0.5179000 0.227042602 0.008867903 FALSE TRUE
ENST00000423312 ENSG00000066735 HEK293_OSMI2_2hA HEK293_TMG_2hB KIF26A protein_coding protein_coding 10.03922 14.90609 6.570928 1.710019 0.1377457 -1.180506 1.6250762 0.9744159 3.0536184 0.4897942 0.6116813 1.637897 0.19025000 0.05993333 0.4635333 0.4036000 0.008867903 0.008867903 FALSE TRUE
MSTRG.10248.1 ENSG00000066735 HEK293_OSMI2_2hA HEK293_TMG_2hB KIF26A protein_coding   10.03922 14.90609 6.570928 1.710019 0.1377457 -1.180506 0.1367403 0.0000000 0.7907342 0.0000000 0.7907342 6.323252 0.01844583 0.00000000 0.1193333 0.1193333 0.749584157 0.008867903 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000066735 E001 0.9587345 0.4090092488 2.769351e-01 4.164347e-01 14 104136546 104136602 57 + 0.480 0.153 -2.261
ENSG00000066735 E002 0.1515154 0.0432561572 1.937022e-01   14 104137676 104138383 708 + 0.178 0.000 -11.204
ENSG00000066735 E003 4.2538821 0.0139167179 3.570452e-03 1.192902e-02 14 104138587 104138594 8 + 0.933 0.520 -1.713
ENSG00000066735 E004 12.5868564 0.0266395828 2.357136e-08 2.709663e-07 14 104138595 104138764 170 + 1.444 0.800 -2.335
ENSG00000066735 E005 0.8470867 0.0142678476 1.506969e-01 2.626193e-01 14 104138853 104138913 61 + 0.401 0.152 -1.853
ENSG00000066735 E006 0.2214452 0.0373799445 1.000000e+00   14 104139040 104139042 3 + 0.000 0.083 9.016
ENSG00000066735 E007 43.3589289 0.0018456337 1.489575e-09 2.138493e-08 14 104139043 104139288 246 + 1.804 1.474 -1.123
ENSG00000066735 E008 119.7509696 0.0003055403 2.251152e-06 1.731312e-05 14 104152015 104152461 447 + 2.120 1.970 -0.503
ENSG00000066735 E009 61.8877851 0.0112037221 3.067009e-01 4.488807e-01 14 104157755 104157942 188 + 1.768 1.719 -0.165
ENSG00000066735 E010 64.8156632 0.0007187665 4.175770e-01 5.609115e-01 14 104166859 104167048 190 + 1.772 1.745 -0.091
ENSG00000066735 E011 79.9926786 0.0003815669 1.747060e-01 2.943233e-01 14 104171723 104171935 213 + 1.877 1.831 -0.155
ENSG00000066735 E012 56.5952485 0.0100254486 7.329939e-03 2.207687e-02 14 104172575 104172668 94 + 1.818 1.650 -0.570
ENSG00000066735 E013 115.3583316 0.0012463566 7.469807e-03 2.243642e-02 14 104172977 104173239 263 + 2.065 1.977 -0.297
ENSG00000066735 E014 88.2973279 0.0146313852 7.641620e-01 8.473012e-01 14 104173330 104173513 184 + 1.894 1.887 -0.023
ENSG00000066735 E015 90.0151983 0.0003386486 5.895960e-02 1.248529e-01 14 104173706 104173868 163 + 1.942 1.878 -0.215
ENSG00000066735 E016 92.0583113 0.0118711228 3.259260e-02 7.698767e-02 14 104174148 104174310 163 + 1.987 1.872 -0.385
ENSG00000066735 E017 82.8784931 0.0070400389 1.153368e-01 2.133414e-01 14 104174982 104175136 155 + 1.908 1.840 -0.229
ENSG00000066735 E018 926.1944563 0.0006247271 3.345289e-02 7.868235e-02 14 104175137 104177898 2762 + 2.867 2.914 0.157
ENSG00000066735 E019 99.4443607 0.0002809868 3.749197e-08 4.143231e-07 14 104178550 104178755 206 + 1.733 1.987 0.857
ENSG00000066735 E020 97.7427896 0.0024178773 2.343565e-04 1.110009e-03 14 104179236 104179386 151 + 1.783 1.975 0.646
ENSG00000066735 E021 636.3652195 0.0046659345 1.075597e-03 4.220002e-03 14 104179609 104180894 1286 + 2.655 2.771 0.388