ENSG00000066427

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000340660 ENSG00000066427 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN3 protein_coding protein_coding 7.855199 3.369453 8.83066 0.2445792 0.4514213 1.387364 1.2049261 1.1474988 0.5840747 0.57378968 0.3402389 -0.9622944 0.17639167 0.35516667 0.06910000 -0.286066667 6.703770e-01 3.095998e-09 FALSE TRUE
ENST00000359366 ENSG00000066427 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN3 protein_coding nonsense_mediated_decay 7.855199 3.369453 8.83066 0.2445792 0.4514213 1.387364 0.7091047 0.0000000 1.1150417 0.00000000 0.1053156 6.8138347 0.06913333 0.00000000 0.12733333 0.127333333 3.095998e-09 3.095998e-09 FALSE TRUE
ENST00000502250 ENSG00000066427 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN3 protein_coding protein_coding 7.855199 3.369453 8.83066 0.2445792 0.4514213 1.387364 0.3007972 0.0000000 0.2982087 0.00000000 0.1549276 4.9458358 0.05086250 0.00000000 0.03310000 0.033100000 3.541673e-01 3.095998e-09 FALSE TRUE
ENST00000503767 ENSG00000066427 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN3 protein_coding protein_coding 7.855199 3.369453 8.83066 0.2445792 0.4514213 1.387364 0.5400464 0.4499632 0.8532532 0.44996317 0.4460181 0.9082654 0.08579583 0.12686667 0.09456667 -0.032300000 7.300450e-01 3.095998e-09 FALSE TRUE
ENST00000504047 ENSG00000066427 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN3 protein_coding processed_transcript 7.855199 3.369453 8.83066 0.2445792 0.4514213 1.387364 1.3016212 0.0000000 0.7309916 0.00000000 0.7309916 6.2113853 0.11415833 0.00000000 0.07530000 0.075300000 9.781475e-01 3.095998e-09 FALSE FALSE
ENST00000511362 ENSG00000066427 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN3 protein_coding processed_transcript 7.855199 3.369453 8.83066 0.2445792 0.4514213 1.387364 0.8558127 0.6255057 1.4576259 0.34655578 0.1104933 1.2075073 0.12224583 0.18450000 0.16673333 -0.017766667 9.715998e-01 3.095998e-09 FALSE TRUE
ENST00000553309 ENSG00000066427 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN3 protein_coding processed_transcript 7.855199 3.369453 8.83066 0.2445792 0.4514213 1.387364 0.4534525 0.2396049 0.2624112 0.23960492 0.2624112 0.1261398 0.05180000 0.06516667 0.02703333 -0.038133333 8.923881e-01 3.095998e-09 FALSE TRUE
ENST00000554592 ENSG00000066427 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN3 protein_coding protein_coding 7.855199 3.369453 8.83066 0.2445792 0.4514213 1.387364 0.1811552 0.0000000 0.9563737 0.00000000 0.1807671 6.5945094 0.02183750 0.00000000 0.11066667 0.110666667 5.489503e-06 3.095998e-09 FALSE TRUE
ENST00000644486 ENSG00000066427 HEK293_OSMI2_2hA HEK293_TMG_2hB ATXN3 protein_coding protein_coding 7.855199 3.369453 8.83066 0.2445792 0.4514213 1.387364 0.6667035 0.3788733 0.9868098 0.05951611 0.1464513 1.3580183 0.08637917 0.11100000 0.11393333 0.002933333 1.000000e+00 3.095998e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000066427 E001 0.2966881 0.0290785164 5.685773e-01   14 92044496 92044774 279 - 0.154 0.000 -9.972
ENSG00000066427 E002 0.3697384 0.0274424043 5.420157e-02 1.166007e-01 14 92044775 92044797 23 - 0.000 0.300 13.301
ENSG00000066427 E003 0.3697384 0.0274424043 5.420157e-02 1.166007e-01 14 92044798 92044805 8 - 0.000 0.300 13.301
ENSG00000066427 E004 1.6899085 0.0333959443 8.451606e-01 9.036782e-01 14 92044806 92044958 153 - 0.432 0.396 -0.193
ENSG00000066427 E005 0.0000000       14 92046275 92046308 34 -      
ENSG00000066427 E006 1.1384763 0.0225134875 4.772321e-01 6.158323e-01 14 92047896 92047987 92 - 0.268 0.397 0.810
ENSG00000066427 E007 0.4783925 0.0222438986 9.017585e-01 9.414029e-01 14 92047988 92047997 10 - 0.154 0.175 0.224
ENSG00000066427 E008 0.4783925 0.0222438986 9.017585e-01 9.414029e-01 14 92047998 92048089 92 - 0.154 0.175 0.224
ENSG00000066427 E009 0.4439371 0.0215775956 3.247207e-01 4.681366e-01 14 92049603 92049610 8 - 0.215 0.000 -12.267
ENSG00000066427 E010 1.8027273 0.0437383485 8.307074e-01 8.938055e-01 14 92049611 92049814 204 - 0.432 0.474 0.214
ENSG00000066427 E011 1.4143249 0.0295368821 8.407932e-01 9.006334e-01 14 92050341 92050536 196 - 0.358 0.396 0.221
ENSG00000066427 E012 0.3729606 0.0297327289 5.291360e-01 6.619598e-01 14 92050537 92050646 110 - 0.084 0.176 1.226
ENSG00000066427 E013 1.5446221 0.1621422196 9.720942e-01 9.865550e-01 14 92058319 92058468 150 - 0.396 0.395 -0.005
ENSG00000066427 E014 75.4730653 0.0047369959 1.546091e-01 2.678845e-01 14 92058552 92062785 4234 - 1.887 1.818 -0.232
ENSG00000066427 E015 35.3880081 0.0006498284 1.571480e-06 1.250366e-05 14 92062786 92063880 1095 - 1.624 1.299 -1.121
ENSG00000066427 E016 13.9319388 0.0013494360 9.970576e-02 1.900263e-01 14 92063881 92064093 213 - 1.202 1.039 -0.584
ENSG00000066427 E017 29.6503393 0.0007376421 3.237499e-02 7.658162e-02 14 92064094 92064414 321 - 1.417 1.554 0.470
ENSG00000066427 E018 0.4783925 0.0222438986 9.017585e-01 9.414029e-01 14 92070745 92070934 190 - 0.154 0.175 0.224
ENSG00000066427 E019 10.0071388 0.0018088127 6.328565e-01 7.476476e-01 14 92070935 92070937 3 - 1.007 1.058 0.189
ENSG00000066427 E020 20.2953857 0.0009655230 5.293479e-01 6.621462e-01 14 92070938 92071010 73 - 1.291 1.340 0.173
ENSG00000066427 E021 19.7863379 0.0040488421 6.736510e-01 7.792850e-01 14 92071011 92071053 43 - 1.286 1.320 0.119
ENSG00000066427 E022 0.0000000       14 92079415 92079468 54 -      
ENSG00000066427 E023 0.0000000       14 92080541 92080567 27 -      
ENSG00000066427 E024 0.1451727 0.0432897268 1.000000e+00   14 92080953 92080964 12 - 0.084 0.000 -10.761
ENSG00000066427 E025 17.1523721 0.0011475784 9.499685e-01 9.726903e-01 14 92080965 92080987 23 - 1.246 1.241 -0.018
ENSG00000066427 E026 22.1560419 0.0009936785 5.395632e-01 6.708149e-01 14 92080988 92081022 35 - 1.331 1.378 0.161
ENSG00000066427 E027 23.8112995 0.0008743079 2.756494e-02 6.714189e-02 14 92081023 92081057 35 - 1.318 1.475 0.541
ENSG00000066427 E028 17.8893529 0.0201010619 1.407517e-01 2.490473e-01 14 92081058 92081061 4 - 1.196 1.356 0.561
ENSG00000066427 E029 27.1906786 0.0009128695 7.155850e-02 1.459278e-01 14 92082300 92082342 43 - 1.388 1.509 0.418
ENSG00000066427 E030 38.8247825 0.0007054380 6.796908e-02 1.399928e-01 14 92082343 92082466 124 - 1.548 1.650 0.350
ENSG00000066427 E031 33.1457631 0.0006853668 3.354465e-01 4.792342e-01 14 92083126 92083235 110 - 1.495 1.554 0.202
ENSG00000066427 E032 17.8328429 0.0011083015 8.667992e-01 9.182758e-01 14 92083236 92083258 23 - 1.251 1.265 0.049
ENSG00000066427 E033 0.7814233 0.0187076817 6.312155e-01 7.462720e-01 14 92083417 92083503 87 - 0.268 0.175 -0.777
ENSG00000066427 E034 27.1185907 0.0007103579 5.941848e-01 7.164578e-01 14 92088730 92088817 88 - 1.441 1.404 -0.128
ENSG00000066427 E035 5.9002363 0.0343716755 4.444606e-01 5.860242e-01 14 92090379 92090567 189 - 0.771 0.877 0.414
ENSG00000066427 E036 19.9262296 0.0009403157 6.838858e-01 7.869775e-01 14 92093252 92093318 67 - 1.309 1.276 -0.115
ENSG00000066427 E037 0.1515154 0.0430677373 1.000000e+00   14 92093379 92093551 173 - 0.084 0.000 -10.762
ENSG00000066427 E038 0.1515154 0.0430677373 1.000000e+00   14 92093552 92093618 67 - 0.084 0.000 -10.762
ENSG00000066427 E039 0.3332198 0.0318722463 5.287311e-01   14 92093619 92093745 127 - 0.084 0.175 1.224
ENSG00000066427 E040 21.6881584 0.0009181294 5.293928e-01 6.621846e-01 14 92093746 92093831 86 - 1.348 1.299 -0.172
ENSG00000066427 E041 0.0000000       14 92093832 92093850 19 -      
ENSG00000066427 E042 20.5852729 0.0009907374 1.425235e-01 2.514660e-01 14 92096093 92096134 42 - 1.348 1.228 -0.420
ENSG00000066427 E043 15.3438567 0.0014657535 4.546380e-01 5.952467e-01 14 92096135 92096137 3 - 1.213 1.144 -0.246
ENSG00000066427 E044 0.0000000       14 92096277 92096673 397 -      
ENSG00000066427 E045 31.0764028 0.0008349740 3.427872e-01 4.868249e-01 14 92096674 92096838 165 - 1.501 1.437 -0.218
ENSG00000066427 E046 16.4877322 0.0011315117 4.547368e-01 5.953389e-01 14 92106529 92106621 93 - 1.241 1.174 -0.236