ENSG00000066422

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000312938 ENSG00000066422 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB11 protein_coding protein_coding 10.87932 2.442247 18.2695 0.1713064 0.4371033 2.898051 0.588032 0.39899751 1.0146239 0.05044273 0.1167911 1.3249304 0.0745875 0.16206667 0.05583333 -0.1062333 0.0007398322 0.0007398322 FALSE TRUE
ENST00000689142 ENSG00000066422 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB11 protein_coding nonsense_mediated_decay 10.87932 2.442247 18.2695 0.1713064 0.4371033 2.898051 6.487144 1.19719409 11.7968631 0.60167394 0.9239535 3.2898962 0.5677083 0.46020000 0.64420000 0.1840000 0.7309127575 0.0007398322 FALSE TRUE
ENST00000690624 ENSG00000066422 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB11 protein_coding protein_coding 10.87932 2.442247 18.2695 0.1713064 0.4371033 2.898051 0.654648 0.62093253 0.4765654 0.36364302 0.2625981 -0.3748521 0.1372417 0.27790000 0.02680000 -0.2511000 0.5544355978 0.0007398322 FALSE TRUE
ENST00000690651 ENSG00000066422 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB11 protein_coding protein_coding 10.87932 2.442247 18.2695 0.1713064 0.4371033 2.898051 2.319986 0.09202662 3.4500923 0.09202662 0.5381223 5.0837930 0.1437458 0.04276667 0.18950000 0.1467333 0.1511988594 0.0007398322 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000066422 E001 5.013319 0.0034362868 1.162238e-06 9.519349e-06 3 101648889 101649446 558 - 0.435 1.198 3.102
ENSG00000066422 E002 5.121871 0.0049816252 3.332282e-07 3.053549e-06 3 101649447 101649454 8 - 0.435 1.224 3.194
ENSG00000066422 E003 463.625436 0.0278183271 2.734581e-06 2.065705e-05 3 101649455 101651514 2060 - 2.408 2.794 1.284
ENSG00000066422 E004 103.561523 0.0010750034 1.390961e-01 2.468261e-01 3 101651515 101651683 169 - 1.822 1.851 0.099
ENSG00000066422 E005 103.871223 0.0005824435 7.201466e-02 1.466827e-01 3 101652496 101652671 176 - 1.826 1.839 0.045
ENSG00000066422 E006 93.816560 0.0003463731 2.642226e-01 4.021803e-01 3 101652780 101652938 159 - 1.778 1.826 0.161
ENSG00000066422 E007 83.960603 0.0003361419 2.816542e-01 4.215733e-01 3 101654704 101654821 118 - 1.733 1.779 0.157
ENSG00000066422 E008 1.481251 0.0093563492 2.197890e-01 3.501435e-01 3 101655841 101656103 263 - 0.317 0.001 -9.426
ENSG00000066422 E009 84.292451 0.0002961973 5.790622e-01 7.040413e-01 3 101656104 101656248 145 - 1.730 1.806 0.259
ENSG00000066422 E010 106.440620 0.0007522718 2.208875e-02 5.586084e-02 3 101659783 101660028 246 - 1.840 1.826 -0.047
ENSG00000066422 E011 110.243329 0.0013336987 4.914084e-05 2.774095e-04 3 101664538 101664714 177 - 1.866 1.742 -0.415
ENSG00000066422 E012 180.785276 0.0503646663 3.811818e-02 8.757746e-02 3 101664964 101665449 486 - 2.075 2.003 -0.241
ENSG00000066422 E013 110.860017 0.0046253050 4.873020e-05 2.753539e-04 3 101665450 101665808 359 - 1.871 1.720 -0.509
ENSG00000066422 E014 6.711957 0.0024681717 7.932595e-01 8.678114e-01 3 101666035 101666120 86 - 0.706 0.839 0.532
ENSG00000066422 E015 13.432939 0.0027258927 5.245834e-01 6.580679e-01 3 101670613 101671129 517 - 0.963 1.140 0.646
ENSG00000066422 E016 88.253738 0.0002932970 8.644384e-06 5.827323e-05 3 101671130 101671361 232 - 1.778 1.606 -0.581
ENSG00000066422 E017 7.896796 0.0184347180 5.358608e-01 6.676613e-01 3 101671362 101671977 616 - 0.759 0.947 0.731
ENSG00000066422 E018 93.328612 0.0002974534 1.887605e-05 1.177559e-04 3 101671978 101672213 236 - 1.797 1.647 -0.510
ENSG00000066422 E019 3.700721 0.0055561912 4.901780e-01 6.275377e-01 3 101675281 101676604 1324 - 0.541 0.473 -0.330
ENSG00000066422 E020 87.463682 0.0013522829 3.161571e-04 1.445343e-03 3 101676605 101677135 531 - 1.767 1.647 -0.407