ENSG00000066248

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264051 ENSG00000066248 HEK293_OSMI2_2hA HEK293_TMG_2hB NGEF protein_coding protein_coding 4.569178 8.273156 3.450181 0.5325562 0.1308432 -1.259333 1.9942028 4.01589375 0.8986740 0.1461602 0.12505972 -2.1474743 0.403800 0.4872667 0.2586000 -0.2286667 0.0001045127 0.0001045127 FALSE TRUE
ENST00000373552 ENSG00000066248 HEK293_OSMI2_2hA HEK293_TMG_2hB NGEF protein_coding protein_coding 4.569178 8.273156 3.450181 0.5325562 0.1308432 -1.259333 1.4111931 1.96465347 1.7038305 0.4742822 0.30775953 -0.2043751 0.350625 0.2361667 0.5005667 0.2644000 0.1277222308 0.0001045127 FALSE TRUE
ENST00000424488 ENSG00000066248 HEK293_OSMI2_2hA HEK293_TMG_2hB NGEF protein_coding protein_coding 4.569178 8.273156 3.450181 0.5325562 0.1308432 -1.259333 0.9174902 2.18978488 0.4446331 0.5259903 0.25276021 -2.2745878 0.174025 0.2638667 0.1245000 -0.1393667 0.5458636541 0.0001045127 FALSE FALSE
ENST00000461944 ENSG00000066248 HEK293_OSMI2_2hA HEK293_TMG_2hB NGEF protein_coding retained_intron 4.569178 8.273156 3.450181 0.5325562 0.1308432 -1.259333 0.1542395 0.05650292 0.2512599 0.0289578 0.08877622 1.9739959 0.046975 0.0072000 0.0731000 0.0659000 0.0082687283 0.0001045127 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000066248 E001 79.801896 0.0004087815 6.593484e-07 5.694884e-06 2 232878701 232879214 514 - 1.624 1.864 0.811
ENSG00000066248 E002 103.176342 0.0018229537 5.407886e-02 1.163906e-01 2 232879215 232879679 465 - 1.873 1.951 0.259
ENSG00000066248 E003 52.124461 0.0005813950 1.403742e-01 2.485464e-01 2 232881146 232881250 105 - 1.581 1.659 0.264
ENSG00000066248 E004 32.255742 0.0015099164 3.429206e-01 4.869596e-01 2 232882186 232882228 43 - 1.392 1.454 0.216
ENSG00000066248 E005 28.725916 0.0266132082 6.307464e-01 7.458803e-01 2 232882229 232882252 24 - 1.360 1.405 0.154
ENSG00000066248 E006 23.370573 0.0017711235 4.659004e-01 6.054937e-01 2 232882253 232882265 13 - 1.266 1.321 0.192
ENSG00000066248 E007 53.702458 0.0005376304 8.818658e-01 9.283141e-01 2 232883311 232883466 156 - 1.663 1.648 -0.050
ENSG00000066248 E008 58.920521 0.0004306110 4.857151e-01 6.235477e-01 2 232883981 232884144 164 - 1.722 1.680 -0.143
ENSG00000066248 E009 1.356702 0.0121701531 1.179579e-03 4.572137e-03 2 232884924 232885279 356 - 0.694 0.139 -3.388
ENSG00000066248 E010 37.851249 0.0005695269 6.175867e-01 7.353628e-01 2 232885280 232885322 43 - 1.534 1.496 -0.132
ENSG00000066248 E011 34.533299 0.0006488934 7.720376e-01 8.528789e-01 2 232885323 232885369 47 - 1.481 1.455 -0.090
ENSG00000066248 E012 0.000000       2 232885370 232885536 167 -      
ENSG00000066248 E013 39.895625 0.0006061097 7.710743e-01 8.522194e-01 2 232888033 232888107 75 - 1.541 1.516 -0.086
ENSG00000066248 E014 3.650371 0.0135931975 2.718329e-01 4.106404e-01 2 232890934 232890980 47 - 0.430 0.624 0.927
ENSG00000066248 E015 43.950100 0.0010583354 4.239197e-01 5.669550e-01 2 232891358 232891487 130 - 1.527 1.570 0.149
ENSG00000066248 E016 39.642191 0.0051752771 4.500501e-01 5.912215e-01 2 232892898 232893050 153 - 1.562 1.504 -0.198
ENSG00000066248 E017 1.296221 0.0117965568 2.914595e-02 7.028082e-02 2 232894602 232894755 154 - 0.581 0.194 -2.317
ENSG00000066248 E018 41.026296 0.0026917918 3.088262e-02 7.368818e-02 2 232894756 232894916 161 - 1.641 1.499 -0.484
ENSG00000066248 E019 0.000000       2 232901362 232901462 101 -      
ENSG00000066248 E020 0.000000       2 232905645 232905849 205 -      
ENSG00000066248 E021 46.786602 0.0091697362 6.880823e-03 2.091438e-02 2 232920284 232920585 302 - 1.731 1.543 -0.642
ENSG00000066248 E022 19.901818 0.0245331035 3.984187e-03 1.309857e-02 2 232927044 232927186 143 - 1.456 1.153 -1.059
ENSG00000066248 E023 9.091987 0.0048580812 7.497185e-03 2.250439e-02 2 232927893 232928154 262 - 1.144 0.835 -1.148
ENSG00000066248 E024 0.000000       2 232970043 232970213 171 -      
ENSG00000066248 E025 0.000000       2 232970214 232970328 115 -      
ENSG00000066248 E026 0.000000       2 232974623 232974964 342 -      
ENSG00000066248 E027 0.000000       2 233013068 233013256 189 -