ENSG00000066136

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000447388 ENSG00000066136 HEK293_OSMI2_2hA HEK293_TMG_2hB NFYC protein_coding protein_coding 47.43526 67.61345 37.56443 2.642165 1.015995 -0.8477724 27.941351 43.800159 20.153597 1.189742 0.8624594 -1.11951244 0.5783958 0.6485000 0.5365333 -0.11196667 0.003493273 1.771704e-06 FALSE TRUE
ENST00000467203 ENSG00000066136 HEK293_OSMI2_2hA HEK293_TMG_2hB NFYC protein_coding retained_intron 47.43526 67.61345 37.56443 2.642165 1.015995 -0.8477724 7.453657 7.954985 7.888294 1.361636 0.2775443 -0.01213049 0.1650000 0.1170333 0.2099000 0.09286667 0.014547351 1.771704e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000066136 E001 0.0000000       1 40685406 40688380 2975 +      
ENSG00000066136 E002 21.2581041 0.0009352943 5.695467e-01 6.960887e-01 1 40688381 40691647 3267 + 1.264 1.318 0.189
ENSG00000066136 E003 53.8699404 0.0004463205 4.851741e-01 6.231004e-01 1 40691648 40691685 38 + 1.656 1.701 0.151
ENSG00000066136 E004 54.9414936 0.0004343848 6.381454e-01 7.518516e-01 1 40691686 40691688 3 + 1.674 1.707 0.109
ENSG00000066136 E005 71.3911708 0.0003767092 8.359766e-01 8.974477e-01 1 40691689 40691703 15 + 1.810 1.808 -0.007
ENSG00000066136 E006 74.2716566 0.0004659717 2.971180e-01 4.384253e-01 1 40691704 40691715 12 + 1.853 1.814 -0.132
ENSG00000066136 E007 69.0894997 0.0020309157 1.870534e-01 3.100325e-01 1 40691716 40691716 1 + 1.835 1.777 -0.198
ENSG00000066136 E008 126.0981465 0.0039766290 9.551643e-02 1.836602e-01 1 40691717 40691742 26 + 2.101 2.033 -0.229
ENSG00000066136 E009 247.9612019 0.0034568287 1.373989e-03 5.221837e-03 1 40691743 40691837 95 + 2.422 2.313 -0.364
ENSG00000066136 E010 202.0566096 0.0063855278 8.856568e-03 2.592356e-02 1 40691838 40691867 30 + 2.338 2.223 -0.383
ENSG00000066136 E011 0.1472490 0.0432797952 2.199784e-01   1 40692008 40692012 5 + 0.171 0.000 -11.780
ENSG00000066136 E012 0.1472490 0.0432797952 2.199784e-01   1 40692013 40692200 188 + 0.171 0.000 -11.780
ENSG00000066136 E013 0.0000000       1 40692290 40692347 58 +      
ENSG00000066136 E014 0.0000000       1 40695606 40695745 140 +      
ENSG00000066136 E015 2.9320028 0.0292717818 1.176869e-01 2.166477e-01 1 40709357 40709542 186 + 0.728 0.473 -1.136
ENSG00000066136 E016 1.7454684 0.0089901151 2.813555e-02 6.828320e-02 1 40709543 40709616 74 + 0.641 0.274 -1.941
ENSG00000066136 E017 0.0000000       1 40712398 40712469 72 +      
ENSG00000066136 E018 22.4968204 0.0293474753 1.034016e-01 1.956663e-01 1 40712687 40712821 135 + 1.161 1.367 0.722
ENSG00000066136 E019 0.4783925 0.0212653892 2.091414e-01 3.375035e-01 1 40721606 40721704 99 + 0.293 0.086 -2.132
ENSG00000066136 E020 1.3995737 0.0098996352 7.127207e-01 8.093636e-01 1 40723208 40723274 67 + 0.293 0.365 0.453
ENSG00000066136 E021 1.5509873 0.0091320969 1.634225e-01 2.796186e-01 1 40723689 40723792 104 + 0.171 0.440 1.867
ENSG00000066136 E022 0.1482932 0.0415634053 1.000000e+00   1 40734848 40735033 186 + 0.000 0.086 9.518
ENSG00000066136 E023 0.0000000       1 40735315 40735600 286 +      
ENSG00000066136 E024 0.6287620 0.0167481929 4.318427e-01 5.743523e-01 1 40735601 40735691 91 + 0.293 0.158 -1.134
ENSG00000066136 E025 0.9254501 0.0136968632 7.585817e-02 1.528743e-01 1 40735692 40735750 59 + 0.467 0.158 -2.134
ENSG00000066136 E026 0.8158510 0.6555130302 1.000000e+00 1.000000e+00 1 40738834 40738835 2 + 0.171 0.236 0.576
ENSG00000066136 E027 154.2637813 0.0072393291 2.257541e-02 5.687169e-02 1 40738836 40738837 2 + 2.217 2.108 -0.363
ENSG00000066136 E028 354.2386389 0.0039686717 1.726596e-02 4.553659e-02 1 40738838 40738948 111 + 2.555 2.477 -0.258
ENSG00000066136 E029 276.5360557 0.0011272273 1.790423e-02 4.692922e-02 1 40747534 40747605 72 + 2.435 2.377 -0.193
ENSG00000066136 E030 338.7005345 0.0009143289 2.544818e-02 6.280229e-02 1 40749573 40749686 114 + 2.515 2.467 -0.160
ENSG00000066136 E031 247.0288728 0.0001881167 4.801235e-04 2.083981e-03 1 40753151 40753212 62 + 2.400 2.323 -0.257
ENSG00000066136 E032 212.5816155 0.0005579346 6.407345e-07 5.546757e-06 1 40753213 40753246 34 + 2.368 2.239 -0.431
ENSG00000066136 E033 1.6188564 0.0284120126 5.544839e-01 6.834616e-01 1 40757953 40758120 168 + 0.467 0.365 -0.550
ENSG00000066136 E034 160.9806153 0.0015748853 2.121582e-06 1.640788e-05 1 40758121 40758123 3 + 2.265 2.109 -0.521
ENSG00000066136 E035 447.9260496 0.0013107873 3.391119e-04 1.536894e-03 1 40758124 40758294 171 + 2.657 2.578 -0.263
ENSG00000066136 E036 5.7871519 0.0030489950 4.450423e-02 9.929097e-02 1 40758295 40758448 154 + 0.963 0.714 -0.973
ENSG00000066136 E037 364.0588500 0.0001475179 8.465651e-01 9.046509e-01 1 40762888 40762989 102 + 2.506 2.518 0.039
ENSG00000066136 E038 311.8682991 0.0001693500 1.836265e-02 4.792722e-02 1 40762990 40763046 57 + 2.402 2.463 0.203
ENSG00000066136 E039 6.4367482 0.0047901018 7.933929e-01 8.678979e-01 1 40763363 40763489 127 + 0.799 0.841 0.163
ENSG00000066136 E040 1.3296333 0.0096747291 4.274782e-03 1.391342e-02 1 40766090 40766231 142 + 0.641 0.158 -2.941
ENSG00000066136 E041 177.3474440 0.0002406012 6.491494e-04 2.713040e-03 1 40766596 40766601 6 + 2.119 2.231 0.374
ENSG00000066136 E042 351.9992366 0.0001978468 6.500301e-03 1.992400e-02 1 40766602 40766685 84 + 2.449 2.516 0.221
ENSG00000066136 E043 215.3005043 0.0025148639 8.132810e-01 8.815996e-01 1 40766686 40766703 18 + 2.274 2.290 0.053
ENSG00000066136 E044 6.3711681 0.0194693103 1.325791e-04 6.702079e-04 1 40766704 40766917 214 + 1.131 0.631 -1.932
ENSG00000066136 E045 17.6981015 0.0010418232 3.540146e-01 4.982984e-01 1 40766918 40766974 57 + 1.275 1.203 -0.254
ENSG00000066136 E046 7.5658215 0.1377347718 7.638400e-03 2.286923e-02 1 40766975 40767243 269 + 1.188 0.711 -1.801
ENSG00000066136 E047 3.4924294 0.0049915942 4.935686e-01 6.305684e-01 1 40768646 40768729 84 + 0.686 0.586 -0.432
ENSG00000066136 E048 17.3563628 0.0194390057 1.719892e-04 8.437652e-04 1 40768730 40769355 626 + 1.462 1.077 -1.352
ENSG00000066136 E049 321.0812286 0.0017596557 2.781553e-02 6.765188e-02 1 40769356 40769415 60 + 2.406 2.479 0.245
ENSG00000066136 E050 3.7859944 0.0041041371 9.326861e-01 9.616084e-01 1 40770323 40770396 74 + 0.641 0.633 -0.032
ENSG00000066136 E051 76.8081525 0.0005666120 1.086613e-01 2.035593e-01 1 40770397 40770615 219 + 1.780 1.861 0.274
ENSG00000066136 E052 55.6145779 0.0004295432 2.122844e-03 7.604978e-03 1 40770616 40770708 93 + 1.570 1.746 0.599
ENSG00000066136 E053 187.1732723 0.0026625626 2.104337e-03 7.547102e-03 1 40770709 40770711 3 + 2.132 2.259 0.427
ENSG00000066136 E054 1013.5587248 0.0029849267 9.016480e-08 9.228727e-07 1 40770712 40771383 672 + 2.844 2.994 0.499
ENSG00000066136 E055 296.3633534 0.0019338379 5.999057e-11 1.097117e-09 1 40771384 40771603 220 + 2.257 2.476 0.730