ENSG00000066044

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000407627 ENSG00000066044 HEK293_OSMI2_2hA HEK293_TMG_2hB ELAVL1 protein_coding protein_coding 111.5395 136.032 104.4235 6.254816 1.625643 -0.3814677 25.87912 39.29775 20.59251 1.105724 0.584376 -0.9319935 0.2284875 0.2897333 0.1971333 -0.09260000 5.547912e-06 5.547912e-06 FALSE  
ENST00000596459 ENSG00000066044 HEK293_OSMI2_2hA HEK293_TMG_2hB ELAVL1 protein_coding protein_coding 111.5395 136.032 104.4235 6.254816 1.625643 -0.3814677 82.41398 93.48239 80.54537 5.988476 1.102034 -0.2148682 0.7424542 0.6861667 0.7715333 0.08536667 5.401272e-03 5.547912e-06 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000066044 E001 5.4631644 0.1062163425 1.698258e-01 2.879916e-01 19 7943984 7948551 4568 - 0.589 0.869 1.148
ENSG00000066044 E002 413.8441276 0.0034846891 4.873691e-02 1.069235e-01 19 7958573 7960916 2344 - 2.614 2.582 -0.107
ENSG00000066044 E003 140.8044743 0.0026184112 3.048347e-14 9.337109e-13 19 7960917 7961612 696 - 2.279 2.019 -0.872
ENSG00000066044 E004 3447.2309948 0.0015322437 3.834998e-21 2.801886e-19 19 7961613 7963158 1546 - 3.385 3.568 0.609
ENSG00000066044 E005 2232.8459928 0.0003533277 6.796278e-02 1.399839e-01 19 7963159 7963629 471 - 3.315 3.330 0.050
ENSG00000066044 E006 1659.0405002 0.0003149704 7.086312e-02 1.447824e-01 19 7963630 7963807 178 - 3.188 3.202 0.047
ENSG00000066044 E007 1742.7557284 0.0002122989 8.582468e-06 5.790942e-05 19 7967565 7967790 226 - 3.225 3.214 -0.037
ENSG00000066044 E008 13.1892800 0.0013516742 2.328131e-01 3.657717e-01 19 7972888 7973558 671 - 1.177 1.098 -0.284
ENSG00000066044 E009 7.6393688 0.0066536606 2.952244e-01 4.363483e-01 19 7973559 7973625 67 - 0.967 0.870 -0.364
ENSG00000066044 E010 1346.5377789 0.0001631310 1.068516e-11 2.215466e-10 19 7973725 7973878 154 - 3.132 3.092 -0.132
ENSG00000066044 E011 957.7628640 0.0011346873 1.941740e-04 9.389611e-04 19 7981083 7981186 104 - 2.982 2.945 -0.124
ENSG00000066044 E012 743.4590717 0.0017512744 7.357152e-04 3.027901e-03 19 7991644 7991831 188 - 2.877 2.833 -0.147
ENSG00000066044 E013 0.3332198 0.0303309499 6.844790e-01   19 8002052 8002203 152 - 0.150 0.099 -0.689
ENSG00000066044 E014 0.1817044 0.0401649101 8.037587e-01   19 8005458 8005494 37 - 0.000 0.099 8.226
ENSG00000066044 E015 61.9950453 0.0034790540 8.298271e-07 7.013216e-06 19 8005495 8005659 165 - 1.907 1.680 -0.767
ENSG00000066044 E016 4.5457470 0.0361063584 8.810267e-01 9.277370e-01 19 8005937 8006002 66 - 0.711 0.711 0.002
ENSG00000066044 E017 3.0645137 0.0058103453 7.887156e-01 8.647329e-01 19 8006003 8006261 259 - 0.542 0.610 0.311