ENSG00000066027

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261461 ENSG00000066027 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP2R5A protein_coding protein_coding 11.91959 7.537258 14.815 0.6730369 0.370398 0.9740074 5.632728 1.634335 9.028444 0.2453265 0.5643447 2.4585703 0.4489667 0.2256667 0.6097333 0.38406667 1.381064e-04 1.2504e-08 FALSE TRUE
ENST00000537030 ENSG00000066027 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP2R5A protein_coding protein_coding 11.91959 7.537258 14.815 0.6730369 0.370398 0.9740074 2.820678 1.665080 1.818333 0.8372509 0.9795178 0.1262990 0.2215208 0.2045667 0.1215000 -0.08306667 9.252009e-01 1.2504e-08 FALSE TRUE
MSTRG.3017.2 ENSG00000066027 HEK293_OSMI2_2hA HEK293_TMG_2hB PPP2R5A protein_coding   11.91959 7.537258 14.815 0.6730369 0.370398 0.9740074 2.967127 3.946375 3.273796 0.3457919 0.1871001 -0.2688149 0.2894667 0.5272000 0.2206333 -0.30656667 1.250400e-08 1.2504e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000066027 E001 44.6267595 0.0348807403 4.702934e-04 2.046436e-03 1 212285410 212285960 551 + 1.735 1.359 -1.285
ENSG00000066027 E002 50.3578650 0.0004457515 8.184404e-05 4.367390e-04 1 212285961 212286291 331 + 1.750 1.557 -0.653
ENSG00000066027 E003 0.0000000       1 212301806 212301862 57 +      
ENSG00000066027 E004 0.1451727 0.0431217997 1.000000e+00   1 212301863 212302092 230 + 0.085 0.000 -8.796
ENSG00000066027 E005 1.6563967 0.0439838771 7.470280e-01 8.346487e-01 1 212306783 212306900 118 + 0.437 0.390 -0.255
ENSG00000066027 E006 0.3332198 0.0308638508 5.770344e-01   1 212313767 212313816 50 + 0.085 0.172 1.166
ENSG00000066027 E007 0.2955422 0.0288068326 5.813796e-01   1 212319377 212319545 169 + 0.085 0.172 1.163
ENSG00000066027 E008 74.0456847 0.0003615994 8.983520e-05 4.742124e-04 1 212329135 212329331 197 + 1.904 1.752 -0.509
ENSG00000066027 E009 78.3783153 0.0057914217 3.375357e-03 1.136519e-02 1 212333497 212333598 102 + 1.927 1.791 -0.456
ENSG00000066027 E010 0.1451727 0.0431217997 1.000000e+00   1 212333952 212334024 73 + 0.085 0.000 -8.796
ENSG00000066027 E011 74.3785529 0.0003497870 1.601669e-02 4.277296e-02 1 212342188 212342280 93 + 1.887 1.805 -0.275
ENSG00000066027 E012 83.7790695 0.0003158769 3.194480e-01 4.625369e-01 1 212345803 212345933 131 + 1.915 1.898 -0.059
ENSG00000066027 E013 53.0106068 0.0004545843 4.372043e-01 5.792101e-01 1 212347347 212347406 60 + 1.718 1.701 -0.059
ENSG00000066027 E014 74.9850761 0.0004041018 8.557176e-02 1.683243e-01 1 212348389 212348497 109 + 1.879 1.828 -0.173
ENSG00000066027 E015 60.6244006 0.0004164871 5.253134e-03 1.662002e-02 1 212349189 212349242 54 + 1.809 1.697 -0.381
ENSG00000066027 E016 66.1400435 0.0019335545 2.648873e-02 6.492871e-02 1 212356626 212356676 51 + 1.836 1.745 -0.306
ENSG00000066027 E017 96.1827561 0.0009320605 2.989974e-02 7.176537e-02 1 212356950 212357069 120 + 1.989 1.924 -0.219
ENSG00000066027 E018 119.3302230 0.0002650262 2.294131e-01 3.617371e-01 1 212357157 212357284 128 + 2.067 2.050 -0.060
ENSG00000066027 E019 102.9091057 0.0004988224 2.342342e-01 3.674367e-01 1 212358686 212358787 102 + 2.004 1.982 -0.073
ENSG00000066027 E020 419.8966494 0.0015167367 6.936540e-30 1.159847e-27 1 212360638 212361853 1216 + 2.503 2.749 0.819