ENSG00000065911

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000394053 ENSG00000065911 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFD2 protein_coding protein_coding 87.96807 43.46926 121.6647 8.459193 1.44678 1.484629 29.125234 22.089165 36.066671 5.5044790 1.820968 0.7070743 0.3668375 0.49853333 0.2963667 -0.20216667 1.950453e-06 3.530843e-07 FALSE TRUE
ENST00000462026 ENSG00000065911 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFD2 protein_coding protein_coding 87.96807 43.46926 121.6647 8.459193 1.44678 1.484629 47.100725 14.881776 66.349250 1.8466968 1.511202 2.1557818 0.4910042 0.35076667 0.5452667 0.19450000 3.530843e-07 3.530843e-07 FALSE FALSE
ENST00000470592 ENSG00000065911 HEK293_OSMI2_2hA HEK293_TMG_2hB MTHFD2 protein_coding nonsense_mediated_decay 87.96807 43.46926 121.6647 8.459193 1.44678 1.484629 5.187764 3.902312 7.818372 0.5230489 1.004121 1.0006910 0.0671250 0.09196667 0.0641000 -0.02786667 2.385580e-01 3.530843e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000065911 E001 0.0000000       2 74186172 74186212 41 +      
ENSG00000065911 E002 0.0000000       2 74186213 74186464 252 +      
ENSG00000065911 E003 0.1451727 0.0441811554 1.000000e+00   2 74186465 74187120 656 + 0.090 0.000 -7.910
ENSG00000065911 E004 0.0000000       2 74187121 74187382 262 +      
ENSG00000065911 E005 0.1451727 0.0441811554 1.000000e+00   2 74187383 74187457 75 + 0.090 0.000 -9.183
ENSG00000065911 E006 8.1387747 0.0020018853 5.839059e-01 7.079268e-01 2 74198610 74198612 3 + 0.969 0.944 -0.093
ENSG00000065911 E007 8.2870678 0.0020262799 7.169367e-01 8.124560e-01 2 74198613 74198613 1 + 0.969 0.966 -0.010
ENSG00000065911 E008 8.2870678 0.0020262799 7.169367e-01 8.124560e-01 2 74198614 74198614 1 + 0.969 0.966 -0.010
ENSG00000065911 E009 8.4343169 0.0019820928 6.461510e-01 7.581342e-01 2 74198615 74198615 1 + 0.980 0.966 -0.050
ENSG00000065911 E010 8.5826100 0.0020299177 7.801159e-01 8.586736e-01 2 74198616 74198616 1 + 0.980 0.987 0.028
ENSG00000065911 E011 113.5272099 0.0014589431 1.661514e-07 1.618099e-06 2 74198617 74198628 12 + 2.108 1.937 -0.576
ENSG00000065911 E012 347.4615007 0.0027095717 1.742947e-05 1.095832e-04 2 74198629 74198742 114 + 2.571 2.460 -0.368
ENSG00000065911 E013 32.1350657 0.0007469321 5.769360e-05 3.200711e-04 2 74201382 74201399 18 + 1.584 1.353 -0.794
ENSG00000065911 E014 50.1636618 0.0042729430 5.188156e-05 2.911031e-04 2 74201400 74201479 80 + 1.769 1.548 -0.749
ENSG00000065911 E015 8.1063232 0.0023247623 1.064305e-03 4.181794e-03 2 74201480 74205704 4225 + 1.056 0.669 -1.500
ENSG00000065911 E016 640.4995001 0.0016877747 1.514422e-08 1.805668e-07 2 74205705 74205889 185 + 2.834 2.729 -0.351
ENSG00000065911 E017 2.8409591 0.0053238056 5.711633e-02 1.216636e-01 2 74205890 74205981 92 + 0.671 0.376 -1.424
ENSG00000065911 E018 3.3215296 0.0050170299 4.503810e-03 1.455334e-02 2 74205982 74206088 107 + 0.749 0.283 -2.330
ENSG00000065911 E019 0.9662231 0.1218290616 3.839843e-01 5.281803e-01 2 74206384 74206393 10 + 0.334 0.165 -1.320
ENSG00000065911 E020 1.5992515 0.0819280975 7.822906e-01 8.602240e-01 2 74206394 74206413 20 + 0.418 0.376 -0.231
ENSG00000065911 E021 2.4495751 0.0344572952 1.768316e-01 2.971274e-01 2 74206414 74206606 193 + 0.602 0.376 -1.127
ENSG00000065911 E022 754.9246023 0.0011077179 1.230916e-09 1.792416e-08 2 74207704 74207826 123 + 2.902 2.808 -0.312
ENSG00000065911 E023 821.4874743 0.0001310425 6.320018e-08 6.670986e-07 2 74208569 74208721 153 + 2.920 2.888 -0.107
ENSG00000065911 E024 12.0284219 0.0014526350 6.385943e-02 1.331233e-01 2 74209019 74209941 923 + 1.158 1.007 -0.545
ENSG00000065911 E025 695.6873116 0.0001422335 2.354138e-04 1.114348e-03 2 74209942 74210049 108 + 2.842 2.830 -0.041
ENSG00000065911 E026 630.5019015 0.0001520030 2.796731e-02 6.794814e-02 2 74211199 74211291 93 + 2.792 2.799 0.025
ENSG00000065911 E027 754.2418401 0.0001186483 2.378665e-02 5.937669e-02 2 74211741 74211866 126 + 2.869 2.879 0.032
ENSG00000065911 E028 5.4790365 0.0118409894 1.349250e-02 3.706132e-02 2 74211970 74211996 27 + 0.626 0.988 1.433
ENSG00000065911 E029 6.1979053 0.0036047587 1.025483e-01 1.943919e-01 2 74212075 74212109 35 + 0.749 0.987 0.916
ENSG00000065911 E030 3.7695496 0.0048061241 1.569993e-02 4.206460e-02 2 74212110 74212128 19 + 0.488 0.870 1.628
ENSG00000065911 E031 3361.5475432 0.0007397464 5.347869e-36 1.391673e-33 2 74214079 74217565 3487 + 3.455 3.602 0.488