ENSG00000065559

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000353533 ENSG00000065559 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP2K4 protein_coding protein_coding 12.06846 4.897198 22.86483 0.2589233 1.771473 2.22079 7.9594905 3.2810309 13.2077826 0.2748189 0.9885152 2.0058687 0.62417917 0.6788667 0.58606667 -0.092800000 7.008668e-01 2.658573e-14 FALSE TRUE
ENST00000415385 ENSG00000065559 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP2K4 protein_coding protein_coding 12.06846 4.897198 22.86483 0.2589233 1.771473 2.22079 1.1482086 0.0000000 3.6373886 0.0000000 0.4347306 8.5107201 0.08942917 0.0000000 0.15950000 0.159500000 2.658573e-14 2.658573e-14 FALSE TRUE
ENST00000538465 ENSG00000065559 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP2K4 protein_coding nonsense_mediated_decay 12.06846 4.897198 22.86483 0.2589233 1.771473 2.22079 0.2231448 0.2989126 0.0000000 0.2989126 0.0000000 -4.9491267 0.05315833 0.0639000 0.00000000 -0.063900000 5.759698e-01 2.658573e-14 FALSE FALSE
ENST00000582897 ENSG00000065559 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP2K4 protein_coding processed_transcript 12.06846 4.897198 22.86483 0.2589233 1.771473 2.22079 0.3722563 0.0000000 1.9643686 0.0000000 1.9643686 7.6252475 0.01509167 0.0000000 0.07533333 0.075333333 1.000000e+00 2.658573e-14   FALSE
ENST00000602305 ENSG00000065559 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP2K4 protein_coding nonsense_mediated_decay 12.06846 4.897198 22.86483 0.2589233 1.771473 2.22079 0.6415834 1.1484047 0.6633726 0.7737341 0.6633726 -0.7826624 0.08610417 0.2211333 0.02543333 -0.195700000 3.652831e-01 2.658573e-14 FALSE TRUE
ENST00000602811 ENSG00000065559 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP2K4 protein_coding nonsense_mediated_decay 12.06846 4.897198 22.86483 0.2589233 1.771473 2.22079 0.6264415 0.1580773 0.7826310 0.1580773 0.4046447 2.2375243 0.05242083 0.0338000 0.03726667 0.003466667 7.841546e-01 2.658573e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000065559 E001 0.4355181 0.5733006220 1.000000e+00 1.000000e+00 17 12020829 12020833 5 + 0.164 0.000 -10.224
ENSG00000065559 E002 7.6731896 0.0023853448 3.525099e-06 2.598722e-05 17 12020834 12020869 36 + 0.955 0.000 -16.653
ENSG00000065559 E003 8.2854803 0.0019387198 1.373150e-06 1.106881e-05 17 12020870 12020876 7 + 0.984 0.000 -16.741
ENSG00000065559 E004 9.7475889 0.0017769698 1.437941e-07 1.417124e-06 17 12020877 12020886 10 + 1.048 0.000 -16.914
ENSG00000065559 E005 23.1651900 0.0202073873 4.312687e-08 4.712341e-07 17 12020887 12020975 89 + 1.387 0.575 -3.086
ENSG00000065559 E006 17.5290738 0.0233598043 4.537051e-04 1.981353e-03 17 12020976 12021001 26 + 1.253 0.765 -1.811
ENSG00000065559 E007 0.0000000       17 12026887 12026970 84 +      
ENSG00000065559 E008 0.4482035 0.0260359876 6.280277e-01 7.436819e-01 17 12032266 12032298 33 + 0.165 0.000 -12.539
ENSG00000065559 E009 46.3066074 0.0139977216 2.754507e-03 9.535204e-03 17 12054889 12054991 103 + 1.630 1.390 -0.823
ENSG00000065559 E010 0.7406253 0.0153541921 3.037440e-01 4.457074e-01 17 12069657 12069755 99 + 0.248 0.000 -13.432
ENSG00000065559 E011 1.0287806 0.0122440040 1.652201e-01 2.819683e-01 17 12069756 12069849 94 + 0.318 0.000 -13.957
ENSG00000065559 E012 91.4809533 0.0062701318 4.419482e-04 1.935677e-03 17 12081356 12081530 175 + 1.912 1.733 -0.606
ENSG00000065559 E013 57.6959924 0.0022884049 1.533494e-03 5.744940e-03 17 12095575 12095601 27 + 1.714 1.548 -0.562
ENSG00000065559 E014 60.3154465 0.0004144599 1.227100e-02 3.418330e-02 17 12095602 12095694 93 + 1.723 1.618 -0.354
ENSG00000065559 E015 66.6824118 0.0004008325 4.456407e-02 9.939521e-02 17 12107790 12107909 120 + 1.759 1.691 -0.230
ENSG00000065559 E016 61.5147404 0.0004033814 3.509043e-02 8.181778e-02 17 12110375 12110426 52 + 1.725 1.646 -0.267
ENSG00000065559 E017 0.0000000       17 12110427 12110809 383 +      
ENSG00000065559 E018 0.5848434 0.0255479523 4.276665e-01 5.705201e-01 17 12113040 12113232 193 + 0.209 0.000 -12.950
ENSG00000065559 E019 90.4820285 0.0030034721 2.852520e-03 9.830663e-03 17 12113233 12113360 128 + 1.897 1.775 -0.410
ENSG00000065559 E020 0.1817044 0.0412060746 1.040405e-01   17 12124299 12125293 995 + 0.000 0.227 12.484
ENSG00000065559 E021 77.1967573 0.0004039978 1.565339e-02 4.196366e-02 17 12125294 12125371 78 + 1.824 1.743 -0.275
ENSG00000065559 E022 91.4306662 0.0003534209 3.149812e-01 4.577286e-01 17 12129139 12129287 149 + 1.884 1.880 -0.013
ENSG00000065559 E023 53.3983875 0.0004688025 4.172326e-01 5.606000e-01 17 12139839 12139884 46 + 1.634 1.720 0.292
ENSG00000065559 E024 830.5181622 0.0019662332 1.466440e-21 1.114371e-19 17 12141147 12143830 2684 + 2.793 2.962 0.563