ENSG00000065548

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000337859 ENSG00000065548 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H15 protein_coding protein_coding 64.07353 25.4537 104.4616 1.148808 4.388739 2.036597 59.009778 22.548318 96.128299 0.1872059 4.40177455 2.091452 0.91415417 0.8889 0.9199333 0.03103333 0.806238569 3.073864e-05 FALSE  
ENST00000445547 ENSG00000065548 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H15 protein_coding protein_coding 64.07353 25.4537 104.4616 1.148808 4.388739 2.036597 1.014319 2.016271 0.608336 1.1211695 0.05913727 -1.712364 0.02532917 0.0759 0.0058000 -0.07010000 0.000934635 3.073864e-05    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000065548 E001 6.0510408 0.0044163374 4.486253e-01 5.899060e-01 2 186486218 186486252 35 + 0.788 0.706 -0.334
ENSG00000065548 E002 7.9030593 0.0091446080 9.854049e-01 9.949015e-01 2 186486253 186486259 7 + 0.859 0.893 0.129
ENSG00000065548 E003 9.7078509 0.0017473813 8.679455e-01 9.190724e-01 2 186486260 186486262 3 + 0.944 0.962 0.066
ENSG00000065548 E004 11.2537326 0.0023385635 8.755080e-01 9.240363e-01 2 186486263 186486266 4 + 1.002 1.022 0.075
ENSG00000065548 E005 346.4231500 0.0038905123 1.727598e-02 4.555731e-02 2 186486267 186486457 191 + 2.466 2.419 -0.157
ENSG00000065548 E006 0.0000000       2 186486891 186486917 27 +      
ENSG00000065548 E007 0.0000000       2 186486918 186487026 109 +      
ENSG00000065548 E008 0.1482932 0.0413809286 1.093374e-01   2 186487270 186487360 91 + 0.000 0.225 10.907
ENSG00000065548 E009 0.0000000       2 186495207 186495232 26 +      
ENSG00000065548 E010 403.1330693 0.0003159299 5.746439e-06 4.035010e-05 2 186495233 186495334 102 + 2.534 2.473 -0.203
ENSG00000065548 E011 2.5087714 0.0671573567 1.252018e-01 2.274279e-01 2 186499619 186499662 44 + 0.543 0.224 -1.881
ENSG00000065548 E012 436.7016098 0.0001726264 6.773238e-07 5.832854e-06 2 186500182 186500293 112 + 2.570 2.507 -0.210
ENSG00000065548 E013 511.5664719 0.0009374482 3.480803e-04 1.572462e-03 2 186501273 186501425 153 + 2.634 2.593 -0.137
ENSG00000065548 E014 335.9284664 0.0002178507 2.654125e-03 9.235471e-03 2 186502496 186502552 57 + 2.451 2.421 -0.098
ENSG00000065548 E015 275.3239485 0.0001992290 3.903220e-01 5.343064e-01 2 186502553 186502587 35 + 2.355 2.375 0.070
ENSG00000065548 E016 475.4582663 0.0002351647 1.184754e-02 3.316756e-02 2 186504032 186504214 183 + 2.596 2.588 -0.027
ENSG00000065548 E017 11.7144018 0.0016044231 6.572523e-04 2.742006e-03 2 186505091 186505450 360 + 1.093 0.643 -1.744
ENSG00000065548 E018 514.4009171 0.0001459546 6.433627e-04 2.692122e-03 2 186505451 186505597 147 + 2.633 2.611 -0.074
ENSG00000065548 E019 473.4853119 0.0001686545 3.292426e-01 4.727489e-01 2 186505740 186505841 102 + 2.587 2.611 0.081
ENSG00000065548 E020 4.2104230 0.0079202956 1.051043e-02 3.000330e-02 2 186505842 186506091 250 + 0.717 0.225 -2.630
ENSG00000065548 E021 509.7605211 0.0002033123 5.660511e-01 6.930747e-01 2 186506713 186506836 124 + 2.612 2.667 0.182
ENSG00000065548 E022 7.0537679 0.0858528878 9.854916e-01 9.949449e-01 2 186507395 186507474 80 + 0.810 0.864 0.212
ENSG00000065548 E023 1246.8135529 0.0040335171 1.163461e-11 2.395806e-10 2 186508543 186509361 819 + 2.954 3.176 0.735