ENSG00000065491

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373491 ENSG00000065491 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D22B protein_coding protein_coding 10.56001 11.50586 9.299914 0.3529284 0.2085793 -0.3067829 10.0860470 10.7038039 9.100998 0.5744961 0.2830444 -0.2337897 0.95477500 0.9290 0.9782667 0.04926667 1.760314e-01 8.800776e-05 FALSE  
MSTRG.28225.2 ENSG00000065491 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D22B protein_coding   10.56001 11.50586 9.299914 0.3529284 0.2085793 -0.3067829 0.3025869 0.6401489 0.000000 0.2140394 0.0000000 -6.0226983 0.02852917 0.0567 0.0000000 -0.05670000 8.800776e-05 8.800776e-05 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000065491 E001 3.3432098 0.0133064719 2.511908e-02 6.212123e-02 6 37257772 37257784 13 + 0.799 0.490 -1.340
ENSG00000065491 E002 49.2040993 0.0005490128 1.629761e-01 2.790184e-01 6 37257785 37257888 104 + 1.709 1.682 -0.092
ENSG00000065491 E003 65.7872603 0.0012280827 2.131126e-01 3.422183e-01 6 37257889 37257973 85 + 1.824 1.810 -0.047
ENSG00000065491 E004 52.8960572 0.0007794995 6.957006e-02 1.426473e-01 6 37269594 37269650 57 + 1.749 1.703 -0.156
ENSG00000065491 E005 121.0020583 0.0003074752 2.773437e-06 2.091804e-05 6 37279304 37279611 308 + 2.138 2.033 -0.350
ENSG00000065491 E006 87.8115027 0.0003540147 1.287924e-04 6.536469e-04 6 37282185 37282364 180 + 1.997 1.897 -0.333
ENSG00000065491 E007 0.3697384 0.0274424043 2.800413e-01 4.198395e-01 6 37282468 37282504 37 + 0.000 0.204 9.472
ENSG00000065491 E008 55.4745174 0.0027736052 1.684066e-02 4.457937e-02 6 37282882 37282952 71 + 1.788 1.706 -0.277
ENSG00000065491 E009 1.6962498 0.0145512661 3.067710e-01 4.489520e-01 6 37282953 37283013 61 + 0.494 0.343 -0.819
ENSG00000065491 E010 81.4661883 0.0004066207 1.086767e-04 5.620660e-04 6 37284336 37284464 129 + 1.968 1.861 -0.359
ENSG00000065491 E011 58.2346301 0.0004227694 1.324530e-02 3.648751e-02 6 37287007 37287072 66 + 1.805 1.735 -0.237
ENSG00000065491 E012 47.0488276 0.0013685460 9.692962e-01 9.847933e-01 6 37291243 37291292 50 + 1.648 1.693 0.151
ENSG00000065491 E013 47.7675328 0.0005217653 5.210686e-01 6.549685e-01 6 37291293 37291357 65 + 1.675 1.685 0.033
ENSG00000065491 E014 78.0883449 0.0003396429 2.110344e-01 3.397508e-01 6 37312918 37313024 107 + 1.894 1.887 -0.023
ENSG00000065491 E015 79.5191183 0.0003942997 3.062614e-01 4.484211e-01 6 37313816 37313891 76 + 1.898 1.901 0.008
ENSG00000065491 E016 87.8142053 0.0003229891 2.390162e-01 3.729782e-01 6 37316703 37316830 128 + 1.942 1.941 -0.004
ENSG00000065491 E017 80.7682677 0.0007043788 3.078720e-01 4.501049e-01 6 37317111 37317206 96 + 1.853 1.937 0.282
ENSG00000065491 E018 593.2502033 0.0034420771 3.226691e-10 5.225122e-09 6 37331044 37332970 1927 + 2.650 2.834 0.614