ENSG00000065268

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000585809 ENSG00000065268 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR18 protein_coding protein_coding 122.7236 226.3471 71.49353 13.71433 1.401215 -1.662514 92.836758 180.970093 48.808271 10.1133836 1.6816328 -1.8903379 0.73411250 0.79996667 0.68230000 -0.11766667 9.792257e-11 9.792257e-11 FALSE TRUE
ENST00000587001 ENSG00000065268 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR18 protein_coding protein_coding 122.7236 226.3471 71.49353 13.71433 1.401215 -1.662514 11.527510 22.781768 9.465487 0.9331771 0.3273523 -1.2662409 0.09394167 0.10113333 0.13253333 0.03140000 6.172262e-02 9.792257e-11 FALSE TRUE
ENST00000591276 ENSG00000065268 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR18 protein_coding retained_intron 122.7236 226.3471 71.49353 13.71433 1.401215 -1.662514 5.941156 7.033667 4.757325 0.7775590 0.2672838 -0.5631466 0.06040833 0.03103333 0.06646667 0.03543333 5.434042e-07 9.792257e-11 FALSE TRUE
MSTRG.16024.6 ENSG00000065268 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR18 protein_coding   122.7236 226.3471 71.49353 13.71433 1.401215 -1.662514 6.275844 9.257257 3.196879 2.3508224 0.7038721 -1.5309724 0.05243333 0.03993333 0.04473333 0.00480000 9.157083e-01 9.792257e-11 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000065268 E001 2.989362 0.0052760530 1.371950e-03 5.214923e-03 19 982907 983050 144 + 0.869 0.366 -2.272
ENSG00000065268 E002 2.512115 0.0062293198 1.077792e-03 4.227333e-03 19 983162 983202 41 + 0.830 0.300 -2.534
ENSG00000065268 E003 2.325024 0.0064516380 6.401447e-02 1.333756e-01 19 983203 983250 48 + 0.685 0.366 -1.536
ENSG00000065268 E004 1.987449 0.0101746833 7.186999e-01 8.136947e-01 19 983942 983961 20 + 0.465 0.396 -0.368
ENSG00000065268 E005 6.201982 0.0277550761 1.582736e-03 5.902452e-03 19 983962 984108 147 + 1.098 0.648 -1.743
ENSG00000065268 E006 5.420763 0.0034919667 4.488338e-05 2.557761e-04 19 984109 984219 111 + 1.098 0.562 -2.122
ENSG00000065268 E007 4.530784 0.0035334646 5.334135e-02 1.150952e-01 19 984220 984302 83 + 0.869 0.581 -1.185
ENSG00000065268 E008 33.329027 0.0133505127 2.675510e-01 4.058837e-01 19 984303 984331 29 + 1.492 1.394 -0.340
ENSG00000065268 E009 51.816788 0.0097895043 4.182558e-02 9.440654e-02 19 984332 984340 9 + 1.717 1.569 -0.505
ENSG00000065268 E010 109.130195 0.0019867915 8.655879e-01 9.174228e-01 19 984341 984344 4 + 1.912 1.919 0.024
ENSG00000065268 E011 205.916252 0.0017683277 8.439632e-01 9.028432e-01 19 984345 984357 13 + 2.200 2.192 -0.027
ENSG00000065268 E012 370.257281 0.0031724238 6.385033e-01 7.521282e-01 19 984358 984377 20 + 2.461 2.446 -0.049
ENSG00000065268 E013 1115.478957 0.0018035224 3.899997e-01 5.340272e-01 19 984378 984563 186 + 2.944 2.925 -0.064
ENSG00000065268 E014 1224.417133 0.0004241275 8.279161e-02 1.639193e-01 19 985865 985975 111 + 2.990 2.963 -0.092
ENSG00000065268 E015 1348.509251 0.0002448314 3.625043e-03 1.208201e-02 19 989762 989895 134 + 3.043 3.003 -0.134
ENSG00000065268 E016 14.141342 0.0113365081 1.479373e-03 5.570805e-03 19 989896 989915 20 + 1.319 0.978 -1.224
ENSG00000065268 E017 32.067529 0.0009894233 8.609580e-12 1.812489e-10 19 989916 990103 188 + 1.728 1.276 -1.552
ENSG00000065268 E018 26.043578 0.0008030640 3.291862e-10 5.320649e-09 19 990104 990222 119 + 1.642 1.188 -1.573
ENSG00000065268 E019 1352.012469 0.0012079335 9.134826e-02 1.772368e-01 19 990223 990364 142 + 3.039 3.007 -0.108
ENSG00000065268 E020 71.794367 0.0031526674 1.650304e-12 3.900751e-11 19 990647 990851 205 + 2.017 1.649 -1.242
ENSG00000065268 E021 1417.061114 0.0006416566 1.396105e-01 2.474949e-01 19 990852 990995 144 + 3.052 3.028 -0.078
ENSG00000065268 E022 24.631010 0.0013130624 1.404102e-03 5.319882e-03 19 990996 991080 85 + 1.492 1.240 -0.877
ENSG00000065268 E023 987.330920 0.0008515328 3.631427e-01 5.075756e-01 19 991081 991133 53 + 2.890 2.873 -0.057
ENSG00000065268 E024 625.796772 0.0016167403 9.116664e-01 9.479435e-01 19 991134 991139 6 + 2.680 2.678 -0.007
ENSG00000065268 E025 608.659503 0.0014514802 8.832574e-01 9.293245e-01 19 991140 991145 6 + 2.669 2.666 -0.011
ENSG00000065268 E026 530.567183 0.0007201314 9.633475e-01 9.808577e-01 19 991227 991233 7 + 2.606 2.606 0.002
ENSG00000065268 E027 1177.231544 0.0003790556 9.967873e-02 1.899993e-01 19 991234 991351 118 + 2.930 2.955 0.084
ENSG00000065268 E028 566.665214 0.0007042051 3.424073e-01 4.864048e-01 19 991955 991960 6 + 2.615 2.636 0.069
ENSG00000065268 E029 750.519400 0.0001239031 2.007329e-03 7.248861e-03 19 991961 991994 34 + 2.711 2.762 0.171
ENSG00000065268 E030 1230.638166 0.0002718077 5.408818e-06 3.820455e-05 19 991995 992121 127 + 2.913 2.980 0.222
ENSG00000065268 E031 7.785592 0.0328129507 7.241245e-01 8.177411e-01 19 994014 994019 6 + 0.870 0.816 -0.206
ENSG00000065268 E032 788.181636 0.0004551998 9.776151e-10 1.452826e-08 19 994020 994088 69 + 2.676 2.794 0.394
ENSG00000065268 E033 1224.567507 0.0015713875 5.261996e-04 2.257608e-03 19 994213 994557 345 + 2.903 2.982 0.263
ENSG00000065268 E034 3.915727 0.0046620339 4.612178e-02 1.022627e-01 19 998313 998438 126 + 0.830 0.521 -1.313