ENSG00000065183

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000349139 ENSG00000065183 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR3 protein_coding protein_coding 52.66871 13.3624 85.17447 1.561302 2.278978 2.671332 13.40185 5.846184 19.73942 0.4859389 0.8637296 1.753778 0.3126333 0.4471667 0.2318333 -0.2153333 1.615557e-03 2.404349e-06 FALSE TRUE
ENST00000369441 ENSG00000065183 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR3 protein_coding protein_coding 52.66871 13.3624 85.17447 1.561302 2.278978 2.671332 14.50478 2.072523 23.53851 0.1454195 1.1471894 3.499231 0.2309833 0.1569000 0.2761667 0.1192667 2.404349e-06 2.404349e-06 FALSE FALSE
ENST00000471680 ENSG00000065183 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR3 protein_coding processed_transcript 52.66871 13.3624 85.17447 1.561302 2.278978 2.671332 23.41793 4.962370 39.94311 1.2179437 1.6590115 3.006302 0.4258583 0.3623000 0.4689333 0.1066333 2.830388e-01 2.404349e-06 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000065183 E001 2.251951 0.0063876375 9.090672e-02 1.765798e-01 1 117929720 117929723 4 + 0.538 0.205 -2.022
ENSG00000065183 E002 2.544373 0.0060300490 5.673563e-02 1.210075e-01 1 117929724 117929736 13 + 0.580 0.205 -2.215
ENSG00000065183 E003 2.538030 0.0074941283 5.739326e-02 1.221560e-01 1 117929737 117929738 2 + 0.580 0.205 -2.215
ENSG00000065183 E004 32.439563 0.0007523688 5.210869e-09 6.776384e-08 1 117929739 117929756 18 + 1.555 1.052 -1.764
ENSG00000065183 E005 100.207198 0.0002644866 8.379253e-15 2.788869e-13 1 117929757 117929774 18 + 2.018 1.660 -1.208
ENSG00000065183 E006 122.002471 0.0002742305 9.731128e-15 3.206702e-13 1 117929775 117929782 8 + 2.097 1.780 -1.068
ENSG00000065183 E007 23.396819 0.0078072781 1.533358e-02 4.124521e-02 1 117929783 117929795 13 + 1.383 1.155 -0.800
ENSG00000065183 E008 78.860547 0.0096189384 7.698540e-02 1.546639e-01 1 117929796 117929924 129 + 1.874 1.768 -0.357
ENSG00000065183 E009 291.740072 0.0006186098 2.635094e-22 2.173315e-20 1 117933288 117933389 102 + 2.467 2.194 -0.912
ENSG00000065183 E010 313.967370 0.0058909404 1.348013e-08 1.622059e-07 1 117933390 117933490 101 + 2.494 2.241 -0.846
ENSG00000065183 E011 3.105484 0.0048417047 1.838293e-03 6.721463e-03 1 117933630 117933740 111 + 0.670 0.000 -11.765
ENSG00000065183 E012 405.444346 0.0095696143 6.351312e-05 3.486431e-04 1 117934473 117934682 210 + 2.596 2.395 -0.671
ENSG00000065183 E013 296.140526 0.0008555258 5.028793e-08 5.421327e-07 1 117936769 117936887 119 + 2.450 2.306 -0.481
ENSG00000065183 E014 244.346678 0.0002165782 3.312402e-03 1.118383e-02 1 117938480 117938558 79 + 2.351 2.284 -0.223
ENSG00000065183 E015 290.278586 0.0001539518 9.839709e-04 3.904781e-03 1 117939477 117939572 96 + 2.426 2.358 -0.228
ENSG00000065183 E016 313.659747 0.0001435149 2.907080e-06 2.181744e-05 1 117940827 117940940 114 + 2.466 2.366 -0.334
ENSG00000065183 E017 296.733059 0.0017079518 1.648984e-04 8.131263e-04 1 117941124 117941225 102 + 2.444 2.334 -0.367
ENSG00000065183 E018 264.163807 0.0012737634 1.362678e-02 3.737047e-02 1 117941750 117941847 98 + 2.384 2.319 -0.217
ENSG00000065183 E019 311.122600 0.0002195433 3.108163e-03 1.058479e-02 1 117942437 117942544 108 + 2.455 2.396 -0.194
ENSG00000065183 E020 402.865589 0.0001731916 1.263967e-02 3.506130e-02 1 117943396 117943626 231 + 2.562 2.522 -0.130
ENSG00000065183 E021 225.821662 0.0001844619 1.533453e-01 2.662282e-01 1 117946086 117946179 94 + 2.307 2.280 -0.088
ENSG00000065183 E022 290.810541 0.0011961365 2.046708e-02 5.247495e-02 1 117948405 117948506 102 + 2.423 2.367 -0.190
ENSG00000065183 E023 297.342835 0.0012927323 1.401973e-01 2.482982e-01 1 117949751 117949836 86 + 2.427 2.396 -0.104
ENSG00000065183 E024 357.750432 0.0001742039 1.070578e-02 3.046608e-02 1 117949995 117950130 136 + 2.511 2.467 -0.146
ENSG00000065183 E025 282.489085 0.0002699778 2.427938e-02 6.039067e-02 1 117950834 117950890 57 + 2.408 2.364 -0.149
ENSG00000065183 E026 383.083869 0.0001499476 6.110727e-03 1.889934e-02 1 117951976 117952076 101 + 2.540 2.494 -0.153
ENSG00000065183 E027 400.142893 0.0010491812 3.581643e-01 5.025411e-01 1 117952297 117952408 112 + 2.551 2.539 -0.039
ENSG00000065183 E028 435.119479 0.0001553002 1.783675e-01 2.990930e-01 1 117952528 117952662 135 + 2.574 2.613 0.132
ENSG00000065183 E029 332.874139 0.0004067064 1.459421e-02 3.958068e-02 1 117952946 117952996 51 + 2.450 2.520 0.234
ENSG00000065183 E030 325.490127 0.0008508093 1.232453e-03 4.750738e-03 1 117953476 117953541 66 + 2.433 2.530 0.321
ENSG00000065183 E031 337.595844 0.0015459454 3.385234e-06 2.505460e-05 1 117954007 117954099 93 + 2.434 2.578 0.481
ENSG00000065183 E032 204.796864 0.0035102467 1.077748e-07 1.088243e-06 1 117954580 117954627 48 + 2.191 2.413 0.741
ENSG00000065183 E033 205.586462 0.0022412711 2.796371e-09 3.819221e-08 1 117955315 117955358 44 + 2.193 2.413 0.735
ENSG00000065183 E034 367.541982 0.0015883699 4.604077e-08 5.001310e-07 1 117957068 117957196 129 + 2.463 2.625 0.539
ENSG00000065183 E035 352.544224 0.0001688379 3.535131e-09 4.732334e-08 1 117958910 117959003 94 + 2.453 2.591 0.461
ENSG00000065183 E036 489.433355 0.0001649800 1.555278e-24 1.627860e-22 1 117959292 117959493 202 + 2.577 2.772 0.650
ENSG00000065183 E037 682.710533 0.0035699892 2.335716e-19 1.390180e-17 1 117959494 117966543 7050 + 2.684 2.975 0.970