ENSG00000065057

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000219066 ENSG00000065057 HEK293_OSMI2_2hA HEK293_TMG_2hB NTHL1 protein_coding protein_coding 91.54439 174.7516 48.14343 5.927486 1.037534 -1.859677 16.52715 36.42003 0.00000 15.09868 0.000000 -11.830912 0.1197625 0.2136667 0.0000 -0.2136667 6.858081e-11 6.858081e-11 FALSE TRUE
ENST00000651570 ENSG00000065057 HEK293_OSMI2_2hA HEK293_TMG_2hB NTHL1 protein_coding protein_coding 91.54439 174.7516 48.14343 5.927486 1.037534 -1.859677 58.99526 115.18764 37.78452 20.23554 1.195214 -1.607862 0.6798000 0.6536333 0.7846 0.1309667 4.888516e-01 6.858081e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000065057 E001 0.2214452 0.0384257450 1.000000e+00   16 2039814 2039814 1 - 0.000 0.063 7.941
ENSG00000065057 E002 0.6997360 0.0166320072 4.655329e-01 6.052202e-01 16 2039815 2039817 3 - 0.000 0.209 11.551
ENSG00000065057 E003 1.2511787 0.0106605294 1.886489e-01 3.120380e-01 16 2039818 2039819 2 - 0.000 0.318 12.510
ENSG00000065057 E004 7.1122441 0.0159777790 1.359241e-01 2.423866e-01 16 2039820 2039820 1 - 0.573 0.834 1.089
ENSG00000065057 E005 10.1336798 0.0178101666 2.105282e-01 3.391664e-01 16 2039821 2039822 2 - 0.763 0.959 0.758
ENSG00000065057 E006 52.6145356 0.0066983918 1.929656e-01 3.175065e-01 16 2039823 2039851 29 - 1.512 1.612 0.343
ENSG00000065057 E007 656.3659984 0.0025339322 9.991563e-02 1.903608e-01 16 2039852 2040047 196 - 2.640 2.688 0.158
ENSG00000065057 E008 797.4350483 0.0016910011 2.513609e-01 3.875921e-01 16 2040133 2040238 106 - 2.741 2.769 0.093
ENSG00000065057 E009 22.3459789 0.0008653583 1.926845e-03 6.998990e-03 16 2040239 2040241 3 - 1.443 1.182 -0.910
ENSG00000065057 E010 20.7018855 0.0101670696 6.720482e-04 2.796344e-03 16 2040242 2040468 227 - 1.464 1.143 -1.124
ENSG00000065057 E011 8.8106640 0.0087942167 2.256573e-03 8.019602e-03 16 2042002 2042146 145 - 1.167 0.795 -1.388
ENSG00000065057 E012 10.4887392 0.0188502124 7.975342e-04 3.250655e-03 16 2043484 2043566 83 - 1.242 0.830 -1.513
ENSG00000065057 E013 959.8513961 0.0004931307 4.552159e-01 5.957439e-01 16 2043567 2043726 160 - 2.833 2.846 0.042
ENSG00000065057 E014 1.8057445 0.0085802671 4.773287e-01 6.158929e-01 16 2043727 2043803 77 - 0.485 0.349 -0.735
ENSG00000065057 E015 2.1401713 0.0982090185 9.792526e-02 1.873563e-01 16 2044050 2044079 30 - 0.646 0.323 -1.633
ENSG00000065057 E016 969.1251395 0.0001018474 1.178282e-02 3.301367e-02 16 2044630 2044800 171 - 2.816 2.852 0.120
ENSG00000065057 E017 20.9710275 0.0036340792 5.987637e-01 7.202956e-01 16 2044801 2044803 3 - 1.258 1.207 -0.180
ENSG00000065057 E018 4.3908335 0.0044580986 5.579469e-01 6.862768e-01 16 2044804 2044826 23 - 0.709 0.612 -0.413
ENSG00000065057 E019 1047.4352584 0.0001279612 8.008962e-01 8.731054e-01 16 2046128 2046279 152 - 2.884 2.880 -0.015
ENSG00000065057 E020 932.3342850 0.0020423159 1.395251e-01 2.473713e-01 16 2046280 2046366 87 - 2.860 2.825 -0.115
ENSG00000065057 E021 19.9357086 0.0009789429 1.988144e-02 5.123724e-02 16 2046367 2046391 25 - 1.360 1.150 -0.739
ENSG00000065057 E022 40.2138854 0.0053911843 6.794778e-10 1.040034e-08 16 2046937 2047708 772 - 1.797 1.372 -1.450
ENSG00000065057 E023 763.2582209 0.0028059473 5.981980e-01 7.198502e-01 16 2047709 2047866 158 - 2.756 2.742 -0.047