ENSG00000065054

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000424542 ENSG00000065054 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC9A3R2 protein_coding protein_coding 29.09491 52.73395 15.19629 2.090281 0.1098088 -1.794337 11.723163 14.955240 9.8644784 5.0242693 0.41328395 -0.599839 0.43137917 0.28020000 0.64956667 0.369366667 3.535004e-02 1.652943e-07 FALSE TRUE
ENST00000432365 ENSG00000065054 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC9A3R2 protein_coding protein_coding 29.09491 52.73395 15.19629 2.090281 0.1098088 -1.794337 6.059738 13.382660 2.8219716 0.3838542 0.53104265 -2.241564 0.18847500 0.25510000 0.18540000 -0.069700000 3.695175e-01 1.652943e-07 FALSE TRUE
ENST00000561844 ENSG00000065054 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC9A3R2 protein_coding protein_coding 29.09491 52.73395 15.19629 2.090281 0.1098088 -1.794337 1.760231 2.800948 0.7353420 0.2110167 0.11775083 -1.915082 0.06215833 0.05296667 0.04843333 -0.004533333 8.559213e-01 1.652943e-07 FALSE FALSE
ENST00000563587 ENSG00000065054 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC9A3R2 protein_coding protein_coding 29.09491 52.73395 15.19629 2.090281 0.1098088 -1.794337 6.137484 17.736224 0.2935895 4.6515750 0.03364139 -5.869246 0.17952500 0.33920000 0.01930000 -0.319900000 1.652943e-07 1.652943e-07 FALSE TRUE
ENST00000564033 ENSG00000065054 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC9A3R2 protein_coding retained_intron 29.09491 52.73395 15.19629 2.090281 0.1098088 -1.794337 1.526239 2.040908 0.6544762 0.3951764 0.04788444 -1.625973 0.05791250 0.03896667 0.04303333 0.004066667 8.460773e-01 1.652943e-07 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000065054 E001 4.245843 0.0037626666 2.944451e-06 2.207167e-05 16 2025356 2025666 311 + 1.075 0.424 -2.717
ENSG00000065054 E002 11.739265 0.0015177506 1.415630e-37 4.164391e-35 16 2026902 2026927 26 + 1.655 0.366 -5.059
ENSG00000065054 E003 164.766080 0.0135631852 1.498664e-05 9.567242e-05 16 2026928 2027218 291 + 2.308 2.039 -0.899
ENSG00000065054 E004 3.979228 0.0044853307 2.278479e-02 5.729905e-02 16 2028140 2028196 57 + 0.869 0.521 -1.465
ENSG00000065054 E005 3.245048 0.0048775903 1.668688e-02 4.425022e-02 16 2029527 2029581 55 + 0.830 0.450 -1.662
ENSG00000065054 E006 289.977552 0.0002034688 2.971443e-02 7.139536e-02 16 2029582 2029753 172 + 2.392 2.331 -0.206
ENSG00000065054 E007 169.254394 0.0002696343 4.429922e-01 5.845520e-01 16 2029754 2029782 29 + 2.095 2.115 0.067
ENSG00000065054 E008 3.479278 0.0111846912 1.540260e-01 2.671479e-01 16 2032812 2032917 106 + 0.739 0.499 -1.055
ENSG00000065054 E009 1.364074 0.0101431014 7.112620e-02 1.452118e-01 16 2033264 2033270 7 + 0.551 0.221 -1.949
ENSG00000065054 E010 3.460350 0.0729834292 1.686455e-01 2.864079e-01 16 2033271 2033426 156 + 0.739 0.483 -1.131
ENSG00000065054 E011 1.141582 0.0132738643 8.053448e-01 8.762001e-01 16 2033427 2033432 6 + 0.215 0.262 0.374
ENSG00000065054 E012 7.622237 0.0022436551 3.129316e-01 4.554454e-01 16 2035391 2035693 303 + 0.938 0.799 -0.535
ENSG00000065054 E013 16.142250 0.0169177250 5.964447e-01 7.183888e-01 16 2035981 2036323 343 + 1.178 1.111 -0.239
ENSG00000065054 E014 451.297249 0.0001329002 3.521398e-03 1.178842e-02 16 2036324 2036503 180 + 2.489 2.543 0.182
ENSG00000065054 E015 598.496802 0.0001767615 8.919296e-02 1.739000e-01 16 2036728 2036881 154 + 2.638 2.662 0.080
ENSG00000065054 E016 394.052414 0.0001425205 9.406072e-01 9.666865e-01 16 2036955 2036998 44 + 2.481 2.476 -0.017
ENSG00000065054 E017 430.237804 0.0001489155 9.262683e-01 9.575851e-01 16 2037538 2037600 63 + 2.520 2.512 -0.028
ENSG00000065054 E018 20.382531 0.0009478138 5.908418e-01 7.136595e-01 16 2037757 2037825 69 + 1.178 1.223 0.160
ENSG00000065054 E019 222.779485 0.0002318700 2.315843e-01 3.643621e-01 16 2037826 2037858 33 + 2.203 2.233 0.099
ENSG00000065054 E020 1000.068194 0.0013163326 3.973085e-03 1.306638e-02 16 2037859 2039026 1168 + 2.841 2.890 0.164