ENSG00000064961

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000333651 ENSG00000064961 HEK293_OSMI2_2hA HEK293_TMG_2hB HMG20B protein_coding protein_coding 129.6896 189.0686 81.45549 13.57159 1.872896 -1.214725 87.131469 153.741883 48.713177 13.1998669 1.7297763 -1.6579240 0.63013333 0.81150000 0.59770000 -0.21380000 3.327002e-09 2.887589e-13 FALSE TRUE
ENST00000416526 ENSG00000064961 HEK293_OSMI2_2hA HEK293_TMG_2hB HMG20B protein_coding protein_coding 129.6896 189.0686 81.45549 13.57159 1.872896 -1.214725 7.175459 0.000000 4.325353 0.0000000 2.1682032 8.7600058 0.06692083 0.00000000 0.05433333 0.05433333 1.232240e-01 2.887589e-13 FALSE FALSE
ENST00000470356 ENSG00000064961 HEK293_OSMI2_2hA HEK293_TMG_2hB HMG20B protein_coding retained_intron 129.6896 189.0686 81.45549 13.57159 1.872896 -1.214725 5.062655 3.563581 5.966453 0.7109155 0.4036508 0.7419190 0.04495417 0.01853333 0.07310000 0.05456667 9.836991e-10 2.887589e-13 FALSE FALSE
ENST00000488973 ENSG00000064961 HEK293_OSMI2_2hA HEK293_TMG_2hB HMG20B protein_coding retained_intron 129.6896 189.0686 81.45549 13.57159 1.872896 -1.214725 10.775855 10.525050 9.195698 1.7629464 0.2614349 -0.1945982 0.09187500 0.05520000 0.11296667 0.05776667 4.721368e-05 2.887589e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000064961 E001 0.1451727 4.257452e-02 1.729789e-01   19 3572777 3572799 23 + 0.186 0.000 -11.407
ENSG00000064961 E002 0.1451727 4.257452e-02 1.729789e-01   19 3572800 3572822 23 + 0.186 0.000 -13.342
ENSG00000064961 E003 0.1451727 4.257452e-02 1.729789e-01   19 3572823 3572824 2 + 0.186 0.000 -13.342
ENSG00000064961 E004 1.3693847 1.046942e-02 5.115238e-01 6.465227e-01 19 3572825 3572925 101 + 0.415 0.300 -0.688
ENSG00000064961 E005 5.0219389 3.151061e-03 2.423931e-03 8.534168e-03 19 3572926 3572943 18 + 0.979 0.578 -1.617
ENSG00000064961 E006 7.6684249 2.213019e-03 5.749671e-02 1.223332e-01 19 3572944 3572947 4 + 1.025 0.804 -0.839
ENSG00000064961 E007 58.5400757 4.073789e-03 3.681819e-07 3.344567e-06 19 3572948 3572976 29 + 1.891 1.613 -0.940
ENSG00000064961 E008 77.0368674 2.929247e-03 1.036745e-08 1.273807e-07 19 3572977 3572978 2 + 2.005 1.734 -0.914
ENSG00000064961 E009 136.8429121 4.807777e-03 5.220066e-02 1.130730e-01 19 3572979 3572985 7 + 2.123 2.044 -0.264
ENSG00000064961 E010 161.1360708 7.193228e-03 2.737148e-01 4.127947e-01 19 3572986 3572994 9 + 2.166 2.126 -0.136
ENSG00000064961 E011 92.8632859 1.440127e-02 5.452912e-04 2.329285e-03 19 3572995 3573291 297 + 2.055 1.834 -0.742
ENSG00000064961 E012 370.2841479 8.324738e-03 7.069844e-02 1.445023e-01 19 3573292 3573347 56 + 2.548 2.480 -0.226
ENSG00000064961 E013 301.3395893 8.809944e-03 4.031283e-01 5.469166e-01 19 3573692 3573696 5 + 2.426 2.402 -0.080
ENSG00000064961 E014 384.9386997 6.110994e-03 1.546379e-01 2.679247e-01 19 3573697 3573744 48 + 2.546 2.503 -0.145
ENSG00000064961 E015 518.5578214 2.479399e-03 8.634882e-01 9.159463e-01 19 3573745 3573800 56 + 2.628 2.647 0.062
ENSG00000064961 E016 94.9055746 2.268540e-03 5.315177e-22 4.236198e-20 19 3573801 3573902 102 + 2.177 1.770 -1.366
ENSG00000064961 E017 207.8514484 4.869098e-03 3.645451e-24 3.675465e-22 19 3573903 3574292 390 + 2.519 2.108 -1.369
ENSG00000064961 E018 45.2647268 8.175708e-03 5.898718e-07 5.147135e-06 19 3574293 3574340 48 + 1.817 1.486 -1.124
ENSG00000064961 E019 61.5403140 9.989415e-03 2.420457e-07 2.283380e-06 19 3574341 3574369 29 + 1.947 1.616 -1.117
ENSG00000064961 E020 67.6245984 1.048659e-02 3.352400e-06 2.483972e-05 19 3574370 3574382 13 + 1.967 1.670 -1.002
ENSG00000064961 E021 493.5137497 1.559692e-04 4.557007e-02 1.012397e-01 19 3574383 3574409 27 + 2.580 2.628 0.159
ENSG00000064961 E022 1239.5332795 8.624171e-05 1.745222e-02 4.593829e-02 19 3574410 3574586 177 + 2.988 3.026 0.127
ENSG00000064961 E023 20.9243486 1.054729e-03 7.809631e-11 1.403311e-09 19 3575502 3575539 38 + 1.577 1.091 -1.699
ENSG00000064961 E024 618.3562113 5.700708e-04 5.500830e-01 6.797575e-01 19 3575540 3575541 2 + 2.701 2.723 0.074
ENSG00000064961 E025 1272.4352785 3.760958e-04 2.949259e-01 4.360295e-01 19 3575542 3575650 109 + 3.010 3.037 0.087
ENSG00000064961 E026 645.6729167 4.503062e-04 3.286306e-01 4.721205e-01 19 3575651 3575660 10 + 2.715 2.743 0.096
ENSG00000064961 E027 16.7011156 1.076526e-03 1.410773e-14 4.549996e-13 19 3575661 3575875 215 + 1.559 0.932 -2.221
ENSG00000064961 E028 43.6680743 3.498503e-03 6.223295e-18 3.126451e-16 19 3575876 3576260 385 + 1.897 1.390 -1.725
ENSG00000064961 E029 563.7033426 6.640308e-04 4.426451e-01 5.842324e-01 19 3576261 3576263 3 + 2.657 2.684 0.090
ENSG00000064961 E030 858.2615191 3.717951e-04 1.863416e-01 3.091555e-01 19 3576264 3576307 44 + 2.835 2.867 0.107
ENSG00000064961 E031 21.5498935 8.574162e-04 1.742494e-14 5.549003e-13 19 3576308 3576351 44 + 1.629 1.069 -1.955
ENSG00000064961 E032 40.0565046 5.603208e-04 2.854465e-28 4.140657e-26 19 3576352 3576552 201 + 1.908 1.303 -2.068
ENSG00000064961 E033 967.9262658 9.069836e-05 9.470686e-01 9.707628e-01 19 3576553 3576615 63 + 2.902 2.912 0.034
ENSG00000064961 E034 666.2382371 3.709207e-04 8.985428e-01 9.392978e-01 19 3576616 3576625 10 + 2.737 2.750 0.044
ENSG00000064961 E035 5.5745422 2.975343e-03 4.256835e-04 1.872839e-03 19 3576885 3576891 7 + 1.047 0.600 -1.766
ENSG00000064961 E036 1348.6248181 1.132222e-03 2.752905e-01 4.145738e-01 19 3576892 3577107 216 + 3.029 3.064 0.117
ENSG00000064961 E037 855.4172356 2.830392e-04 1.944902e-02 5.030731e-02 19 3577981 3578113 133 + 2.820 2.867 0.158
ENSG00000064961 E038 21.8375214 6.787738e-03 8.350447e-06 5.648465e-05 19 3578114 3578444 331 + 1.532 1.169 -1.266
ENSG00000064961 E039 1148.2253140 1.869829e-03 7.958103e-07 6.751629e-06 19 3578509 3578701 193 + 2.885 3.015 0.433
ENSG00000064961 E040 1524.5818140 4.467728e-03 5.793107e-06 4.064615e-05 19 3578702 3579088 387 + 2.984 3.145 0.536