ENSG00000064545

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000587406 ENSG00000064545 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM161A protein_coding retained_intron 93.59893 134.6448 61.01562 7.276584 1.23321 -1.141779 11.958568 14.517383 7.117763 0.7331258 1.29467147 -1.0272535 0.12860417 0.10823333 0.11750000 0.009266667 0.9617270705 0.0002197434 TRUE TRUE
ENST00000587583 ENSG00000064545 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM161A protein_coding protein_coding 93.59893 134.6448 61.01562 7.276584 1.23321 -1.141779 22.965196 41.549996 10.445293 5.1762079 0.23747288 -1.9909620 0.23210000 0.30746667 0.17120000 -0.136266667 0.0002197434 0.0002197434 FALSE TRUE
ENST00000587915 ENSG00000064545 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM161A protein_coding protein_coding 93.59893 134.6448 61.01562 7.276584 1.23321 -1.141779 6.373550 5.149005 6.086607 1.8589385 0.50532508 0.2409139 0.07370417 0.03780000 0.09963333 0.061833333 0.0245301361 0.0002197434 FALSE FALSE
ENST00000587985 ENSG00000064545 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM161A protein_coding nonsense_mediated_decay 93.59893 134.6448 61.01562 7.276584 1.23321 -1.141779 12.322415 20.464893 9.333860 3.3533833 1.61357229 -1.1317653 0.12907500 0.15566667 0.15336667 -0.002300000 1.0000000000 0.0002197434 TRUE TRUE
ENST00000592369 ENSG00000064545 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM161A protein_coding protein_coding 93.59893 134.6448 61.01562 7.276584 1.23321 -1.141779 5.999336 7.056904 4.446097 1.0910080 0.06510568 -0.6652979 0.06718750 0.05206667 0.07290000 0.020833333 0.1766237988 0.0002197434   FALSE
MSTRG.16754.4 ENSG00000064545 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM161A protein_coding   93.59893 134.6448 61.01562 7.276584 1.23321 -1.141779 8.594654 14.584821 3.607258 4.5898551 2.43341407 -2.0124882 0.08788333 0.10546667 0.05760000 -0.047866667 0.7020990505 0.0002197434 TRUE TRUE
MSTRG.16754.8 ENSG00000064545 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM161A protein_coding   93.59893 134.6448 61.01562 7.276584 1.23321 -1.141779 10.475836 15.851858 7.323830 1.2138882 1.05345595 -1.1129230 0.10778750 0.11803333 0.11993333 0.001900000 1.0000000000 0.0002197434 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000064545 E001 15.1558815 0.0025941198 2.326687e-05 1.420875e-04 19 19119169 19119614 446 - 1.395 1.025 -1.315
ENSG00000064545 E002 18.3535766 0.0109251182 9.376079e-01 9.647762e-01 19 19119615 19119639 25 - 1.214 1.232 0.065
ENSG00000064545 E003 35.7090428 0.0244431366 8.742261e-01 9.231291e-01 19 19119640 19119648 9 - 1.479 1.510 0.105
ENSG00000064545 E004 67.1048416 0.0221982440 4.769610e-01 6.155618e-01 19 19119649 19119659 11 - 1.706 1.786 0.270
ENSG00000064545 E005 142.9709944 0.0087869927 4.251848e-02 9.566053e-02 19 19119660 19119683 24 - 1.989 2.119 0.435
ENSG00000064545 E006 371.3989943 0.0031860317 1.566385e-02 4.198874e-02 19 19119684 19119737 54 - 2.427 2.523 0.317
ENSG00000064545 E007 508.3780116 0.0022847474 3.175969e-03 1.078504e-02 19 19119738 19119778 41 - 2.561 2.659 0.324
ENSG00000064545 E008 1515.2115628 0.0028784799 1.773869e-04 8.670574e-04 19 19119779 19120183 405 - 3.026 3.134 0.359
ENSG00000064545 E009 638.3561940 0.0008289393 3.876376e-05 2.241386e-04 19 19120765 19120861 97 - 2.657 2.754 0.325
ENSG00000064545 E010 0.7363563 0.0172671820 9.480213e-01 9.713350e-01 19 19120862 19120975 114 - 0.185 0.204 0.180
ENSG00000064545 E011 545.4162346 0.0001412581 6.219927e-04 2.613448e-03 19 19120992 19121083 92 - 2.611 2.681 0.235
ENSG00000064545 E012 601.6120762 0.0001541556 1.683731e-02 4.457113e-02 19 19121084 19121166 83 - 2.668 2.717 0.165
ENSG00000064545 E013 336.8170855 0.0009741759 1.850898e-01 3.076028e-01 19 19121308 19121317 10 - 2.421 2.465 0.148
ENSG00000064545 E014 394.5683005 0.0004707450 1.624112e-01 2.782801e-01 19 19121318 19121341 24 - 2.492 2.532 0.134
ENSG00000064545 E015 546.8791339 0.0006559653 2.339229e-01 3.670426e-01 19 19121342 19121421 80 - 2.641 2.675 0.113
ENSG00000064545 E016 46.0752127 0.0079212748 1.619528e-01 2.776679e-01 19 19121422 19121519 98 - 1.668 1.577 -0.310
ENSG00000064545 E017 10.7287990 0.0023463102 6.218274e-02 1.303192e-01 19 19121520 19121524 5 - 1.137 0.944 -0.706
ENSG00000064545 E018 625.4040807 0.0002306615 4.762328e-01 6.148810e-01 19 19121525 19121656 132 - 2.707 2.728 0.069
ENSG00000064545 E019 295.7809371 0.0020681444 5.199901e-01 6.540294e-01 19 19121657 19121668 12 - 2.374 2.406 0.105
ENSG00000064545 E020 524.9513029 0.0016738743 1.945361e-01 3.194248e-01 19 19121759 19121819 61 - 2.614 2.659 0.150
ENSG00000064545 E021 459.1806353 0.0007066031 3.278343e-01 4.713054e-01 19 19130156 19130191 36 - 2.566 2.597 0.102
ENSG00000064545 E022 514.2999134 0.0001450812 1.184179e-02 3.315438e-02 19 19130192 19130264 73 - 2.667 2.629 -0.126
ENSG00000064545 E023 501.0694399 0.0001256687 7.997948e-06 5.432336e-05 19 19130265 19130307 43 - 2.682 2.610 -0.241
ENSG00000064545 E024 3.7676361 0.2207717211 1.387101e-01 2.463183e-01 19 19130308 19130511 204 - 0.834 0.516 -1.354
ENSG00000064545 E025 650.3735860 0.0002567497 8.614743e-14 2.471121e-12 19 19132352 19132433 82 - 2.827 2.712 -0.383
ENSG00000064545 E026 534.1739773 0.0023471962 4.504197e-07 4.020290e-06 19 19132434 19132508 75 - 2.755 2.623 -0.441
ENSG00000064545 E027 16.0977463 0.0013908547 1.364440e-01 2.431031e-01 19 19132509 19132656 148 - 1.267 1.139 -0.452
ENSG00000064545 E028 343.9433079 0.0027063861 7.690122e-05 4.134139e-04 19 19132657 19132657 1 - 2.555 2.436 -0.397
ENSG00000064545 E029 675.6626093 0.0006781811 4.242985e-05 2.431179e-04 19 19132658 19132754 97 - 2.810 2.741 -0.231
ENSG00000064545 E030 17.0635210 0.0090076273 4.785780e-03 1.533423e-02 19 19132880 19133129 250 - 1.377 1.119 -0.908
ENSG00000064545 E031 456.9940605 0.0024558192 6.211787e-01 7.381879e-01 19 19133130 19133210 81 - 2.593 2.588 -0.017
ENSG00000064545 E032 18.5391642 0.0012995872 1.335800e-03 5.095714e-03 19 19133211 19133306 96 - 1.404 1.146 -0.908
ENSG00000064545 E033 8.3764942 0.0022848424 6.306790e-02 1.318199e-01 19 19133307 19133357 51 - 1.064 0.857 -0.776
ENSG00000064545 E034 9.6689416 0.0025755054 3.362506e-05 1.975306e-04 19 19134477 19134783 307 - 1.241 0.806 -1.605
ENSG00000064545 E035 374.2032980 0.0045273791 1.481124e-01 2.591163e-01 19 19134784 19134887 104 - 2.534 2.491 -0.143
ENSG00000064545 E036 6.8829350 0.0470535359 6.772631e-01 7.820214e-01 19 19134888 19134916 29 - 0.866 0.812 -0.212
ENSG00000064545 E037 3.7956626 0.0044415388 1.215469e-02 3.390119e-02 19 19134917 19134979 63 - 0.867 0.505 -1.532
ENSG00000064545 E038 196.5500423 0.0046505068 2.398666e-01 3.739836e-01 19 19138426 19138456 31 - 2.253 2.212 -0.135
ENSG00000064545 E039 67.8415132 0.0051701525 1.375342e-01 2.446543e-01 19 19138457 19138513 57 - 1.824 1.745 -0.268