ENSG00000063854

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000397353 ENSG00000063854 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGH protein_coding protein_coding 74.69565 118.6624 48.8068 5.749867 0.54959 -1.281535 12.587525 20.99685 6.822471 0.5131789 0.5705916 -1.6203804 0.15608333 0.1774 0.13986667 -0.03753333 1.860289e-01 6.371307e-46 FALSE TRUE
ENST00000564445 ENSG00000063854 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGH protein_coding protein_coding 74.69565 118.6624 48.8068 5.749867 0.54959 -1.281535 27.834904 49.85268 16.279974 6.6698526 0.2618279 -1.6139763 0.39828333 0.4191 0.33353333 -0.08556667 4.176328e-01 6.371307e-46 FALSE TRUE
MSTRG.11800.10 ENSG00000063854 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGH protein_coding   74.69565 118.6624 48.8068 5.749867 0.54959 -1.281535 7.095617 13.46194 3.759679 1.6298782 0.2132201 -1.8374445 0.08956667 0.1138 0.07706667 -0.03673333 1.842379e-01 6.371307e-46 FALSE TRUE
MSTRG.11800.13 ENSG00000063854 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGH protein_coding   74.69565 118.6624 48.8068 5.749867 0.54959 -1.281535 2.004016 0.00000 3.321725 0.0000000 0.2550899 8.3801254 0.03536250 0.0000 0.06820000 0.06820000 6.371307e-46 6.371307e-46 FALSE TRUE
MSTRG.11800.3 ENSG00000063854 HEK293_OSMI2_2hA HEK293_TMG_2hB HAGH protein_coding   74.69565 118.6624 48.8068 5.749867 0.54959 -1.281535 16.675871 21.05166 13.686135 3.8012241 0.6865792 -0.6208503 0.20780417 0.1777 0.28020000 0.10250000 1.209672e-01 6.371307e-46 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000063854 E001 0.0000000       16 1795620 1795720 101 -      
ENSG00000063854 E002 1.4833097 0.4819172955 2.521648e-01 3.884890e-01 16 1806803 1806880 78 - 0.509 0.263 -1.421
ENSG00000063854 E003 2.2548774 0.0835488261 8.263645e-01 8.907365e-01 16 1807577 1807628 52 - 0.426 0.485 0.302
ENSG00000063854 E004 10.0836336 0.0017918203 2.122958e-01 3.412421e-01 16 1807629 1807764 136 - 0.852 1.006 0.581
ENSG00000063854 E005 17.7564059 0.0010771401 1.110240e-01 2.070500e-01 16 1807765 1807948 184 - 1.063 1.221 0.566
ENSG00000063854 E006 46.4679294 0.0005527361 1.613243e-04 7.976614e-04 16 1807949 1808869 921 - 1.386 1.639 0.865
ENSG00000063854 E007 8.2060084 0.0373869415 1.293815e-01 2.333175e-01 16 1808870 1808871 2 - 0.690 0.936 0.971
ENSG00000063854 E008 17.6719269 0.0023198646 8.643675e-02 1.697033e-01 16 1808872 1809025 154 - 1.041 1.217 0.629
ENSG00000063854 E009 13.9306788 0.0467318811 4.782263e-02 1.053100e-01 16 1809026 1809102 77 - 0.817 1.141 1.208
ENSG00000063854 E010 8.2510931 0.0056934401 5.346259e-03 1.687436e-02 16 1809103 1809104 2 - 0.508 0.946 1.818
ENSG00000063854 E011 8.7200224 0.0219692041 9.209201e-02 1.783820e-01 16 1809105 1809106 2 - 0.689 0.949 1.022
ENSG00000063854 E012 31.8120737 0.0006724290 2.486596e-03 8.723994e-03 16 1809107 1809123 17 - 1.233 1.474 0.837
ENSG00000063854 E013 214.1370723 0.0006434271 2.454553e-03 8.628190e-03 16 1809124 1809237 114 - 2.176 2.270 0.316
ENSG00000063854 E014 635.9907701 0.0003824867 3.514375e-04 1.585851e-03 16 1809238 1809382 145 - 2.670 2.736 0.219
ENSG00000063854 E015 48.1574755 0.0092320298 9.767420e-02 1.869458e-01 16 1809383 1809446 64 - 1.507 1.635 0.439
ENSG00000063854 E016 658.2088605 0.0015906545 1.055709e-02 3.010719e-02 16 1809754 1809833 80 - 2.688 2.751 0.209
ENSG00000063854 E017 1.9091847 0.0090798002 2.942477e-02 7.082245e-02 16 1809834 1810887 1054 - 0.000 0.464 11.013
ENSG00000063854 E018 0.0000000       16 1812359 1812419 61 -      
ENSG00000063854 E019 815.2854104 0.0002460227 1.002820e-01 1.909149e-01 16 1816893 1816994 102 - 2.809 2.834 0.084
ENSG00000063854 E020 486.2887596 0.0001992630 1.400352e-01 2.480737e-01 16 1817168 1817188 21 - 2.581 2.609 0.094
ENSG00000063854 E021 718.6495202 0.0002650045 6.945948e-02 1.424631e-01 16 1817189 1817271 83 - 2.749 2.778 0.099
ENSG00000063854 E022 15.0219408 0.0013272484 6.596490e-02 1.366403e-01 16 1818515 1819114 600 - 1.261 1.088 -0.616
ENSG00000063854 E023 669.5089021 0.0005381952 1.119167e-01 2.083783e-01 16 1819115 1819223 109 - 2.718 2.748 0.100
ENSG00000063854 E024 0.5117906 0.2387284679 1.950595e-01 3.200595e-01 16 1819835 1819835 1 - 0.324 0.077 -2.517
ENSG00000063854 E025 2.3447054 0.0258363350 6.497637e-02 1.349971e-01 16 1819836 1819896 61 - 0.689 0.369 -1.538
ENSG00000063854 E026 787.7811376 0.0001491729 1.050532e-03 4.134365e-03 16 1819897 1820014 118 - 2.849 2.801 -0.160
ENSG00000063854 E027 22.9834097 0.0008657590 5.565046e-07 4.880706e-06 16 1821512 1822299 788 - 1.556 1.185 -1.292
ENSG00000063854 E028 602.0645214 0.0004951997 3.001691e-05 1.786277e-04 16 1822300 1822364 65 - 2.756 2.679 -0.256
ENSG00000063854 E029 693.0243688 0.0002945781 6.298011e-08 6.652204e-07 16 1822865 1823037 173 - 2.825 2.737 -0.293
ENSG00000063854 E030 50.5901861 0.0062330275 3.156113e-04 1.443231e-03 16 1826507 1826602 96 - 1.797 1.565 -0.789
ENSG00000063854 E031 7.4190982 0.0043357491 3.492104e-05 2.042131e-04 16 1826603 1826711 109 - 1.173 0.683 -1.864
ENSG00000063854 E032 219.2727155 0.0058184279 8.914757e-03 2.606850e-02 16 1826712 1826940 229 - 2.348 2.232 -0.387
ENSG00000063854 E033 19.3319863 0.0113761795 7.918270e-01 8.668499e-01 16 1826941 1828997 2057 - 1.247 1.219 -0.100