ENSG00000063660

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264039 ENSG00000063660 HEK293_OSMI2_2hA HEK293_TMG_2hB GPC1 protein_coding protein_coding 19.44441 27.68696 15.79272 1.091343 0.4172229 -0.809555 13.8760790 20.0744389 11.1218454 2.4575027 0.1910010 -0.8513854 0.71212083 0.72360000 0.7049667 -0.01863333 9.051458e-01 2.038701e-13 FALSE TRUE
ENST00000455111 ENSG00000063660 HEK293_OSMI2_2hA HEK293_TMG_2hB GPC1 protein_coding protein_coding 19.44441 27.68696 15.79272 1.091343 0.4172229 -0.809555 2.6539257 0.3394781 3.1298495 0.3394781 0.2896147 3.1674213 0.15077917 0.01286667 0.1974667 0.18460000 1.900334e-02 2.038701e-13 FALSE TRUE
ENST00000466624 ENSG00000063660 HEK293_OSMI2_2hA HEK293_TMG_2hB GPC1 protein_coding processed_transcript 19.44441 27.68696 15.79272 1.091343 0.4172229 -0.809555 1.9320473 6.6602614 0.0000000 1.6881304 0.0000000 -9.3815995 0.07935833 0.24163333 0.0000000 -0.24163333 2.038701e-13 2.038701e-13 TRUE TRUE
MSTRG.20161.2 ENSG00000063660 HEK293_OSMI2_2hA HEK293_TMG_2hB GPC1 protein_coding   19.44441 27.68696 15.79272 1.091343 0.4172229 -0.809555 0.1793745 0.0000000 0.8492822 0.0000000 0.2179929 6.4250601 0.01165000 0.00000000 0.0539000 0.05390000 1.991499e-09 2.038701e-13 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000063660 E001 7.9557618 0.0065668047 2.388076e-09 3.302291e-08 2 240435663 240435696 34 + 1.253 0.595 -2.528
ENSG00000063660 E002 106.4614260 0.0029848752 3.843607e-11 7.272144e-10 2 240435697 240436084 388 + 2.139 1.907 -0.777
ENSG00000063660 E003 0.5911836 0.0277671877 2.508641e-01 3.869741e-01 2 240442327 240442506 180 + 0.000 0.239 10.505
ENSG00000063660 E004 0.0000000       2 240452811 240453094 284 +      
ENSG00000063660 E005 1.0350190 0.0427389931 3.720642e-01 5.165432e-01 2 240454838 240455020 183 + 0.156 0.346 1.491
ENSG00000063660 E006 0.9201370 0.0141660280 5.083019e-01 6.437468e-01 2 240457434 240457469 36 + 0.156 0.296 1.173
ENSG00000063660 E007 2.0167830 0.3491972313 9.669083e-01 9.832572e-01 2 240457965 240458117 153 + 0.499 0.436 -0.317
ENSG00000063660 E008 5.0636820 0.0032700121 4.172188e-02 9.422749e-02 2 240458187 240459029 843 + 0.900 0.669 -0.922
ENSG00000063660 E009 130.2308614 0.0047943142 3.353615e-03 1.130263e-02 2 240459030 240459188 159 + 2.136 2.054 -0.274
ENSG00000063660 E010 252.2374562 0.0026524544 9.481476e-02 1.826038e-01 2 240462191 240462582 392 + 2.370 2.367 -0.008
ENSG00000063660 E011 109.8562818 0.0002949930 8.687271e-03 2.550446e-02 2 240463347 240463396 50 + 2.039 1.993 -0.153
ENSG00000063660 E012 175.6898514 0.0002715040 1.285907e-03 4.929906e-03 2 240463397 240463512 116 + 2.240 2.196 -0.144
ENSG00000063660 E013 18.8988333 0.0010007829 4.065653e-07 3.660595e-06 2 240463513 240464615 1103 + 1.472 1.121 -1.229
ENSG00000063660 E014 191.6546660 0.0002386146 1.874654e-02 4.877597e-02 2 240464616 240464746 131 + 2.261 2.245 -0.053
ENSG00000063660 E015 1.0663539 0.0123930495 3.584996e-01 5.029077e-01 2 240464835 240464855 21 + 0.156 0.347 1.494
ENSG00000063660 E016 166.5250057 0.0005079405 5.555324e-01 6.843755e-01 2 240464856 240464975 120 + 2.144 2.210 0.220
ENSG00000063660 E017 218.3706465 0.0022445547 3.152394e-02 7.492586e-02 2 240465077 240465210 134 + 2.320 2.300 -0.065
ENSG00000063660 E018 168.6655089 0.0008269166 3.428964e-02 8.031043e-02 2 240465473 240465526 54 + 2.205 2.187 -0.058
ENSG00000063660 E019 229.6770190 0.0003954862 6.848704e-03 2.083346e-02 2 240465527 240465648 122 + 2.340 2.319 -0.069
ENSG00000063660 E020 292.2523718 0.0001617561 4.497959e-01 5.909983e-01 2 240466058 240466369 312 + 2.411 2.442 0.104
ENSG00000063660 E021 91.4785102 0.0002998199 2.028844e-02 5.208672e-02 2 240466370 240466445 76 + 1.828 1.970 0.477
ENSG00000063660 E022 1143.4958761 0.0050430483 1.162835e-08 1.416875e-07 2 240466446 240468409 1964 + 2.881 3.082 0.669