ENSG00000061455

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000407847 ENSG00000061455 HEK293_OSMI2_2hA HEK293_TMG_2hB PRDM6 protein_coding protein_coding 3.157056 3.177286 2.933737 0.06498619 0.06813121 -0.1146799 0.7763366 0.3624905 1.177365 0.02434558 0.06196272 1.6724878 0.2507292 0.1143000 0.4008 0.2865000 2.137055e-08 2.137055e-08 FALSE TRUE
MSTRG.26820.1 ENSG00000061455 HEK293_OSMI2_2hA HEK293_TMG_2hB PRDM6 protein_coding   3.157056 3.177286 2.933737 0.06498619 0.06813121 -0.1146799 2.0756169 2.4348602 1.474681 0.18006465 0.05098946 -0.7195988 0.6530417 0.7683667 0.5028 -0.2655667 2.106428e-02 2.137055e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000061455 E001 0.3030308 0.3339046446 3.807281e-01   5 123075746 123075803 58 + 0.195 0.000 -9.280
ENSG00000061455 E002 2.7834457 0.1476851590 6.754587e-01 7.807460e-01 5 123089205 123089240 36 + 0.612 0.519 -0.428
ENSG00000061455 E003 3.1564063 0.1051887920 7.109116e-01 8.079818e-01 5 123089241 123089250 10 + 0.642 0.567 -0.332
ENSG00000061455 E004 8.0052598 0.0792118390 8.463810e-01 9.045256e-01 5 123089251 123089448 198 + 0.921 0.962 0.155
ENSG00000061455 E005 7.8630092 0.0022168144 8.628747e-01 9.155825e-01 5 123089449 123089519 71 + 0.950 0.930 -0.075
ENSG00000061455 E006 15.2788787 0.0011446512 1.332395e-01 2.386303e-01 5 123090000 123090606 607 + 1.267 1.138 -0.457
ENSG00000061455 E007 6.3316268 0.0028134718 5.780312e-01 7.031849e-01 5 123099654 123099744 91 + 0.891 0.821 -0.267
ENSG00000061455 E008 3.0206823 0.0960736179 5.138836e-01 6.486572e-01 5 123099745 123099745 1 + 0.513 0.652 0.624
ENSG00000061455 E009 14.4451478 0.0014419162 4.278438e-01 5.706682e-01 5 123099746 123099961 216 + 1.146 1.215 0.245
ENSG00000061455 E010 17.4645007 0.0011524085 6.073200e-01 7.273259e-01 5 123155884 123156011 128 + 1.247 1.287 0.142
ENSG00000061455 E011 17.3683155 0.0012565504 1.179465e-01 2.169863e-01 5 123159514 123159638 125 + 1.203 1.327 0.436
ENSG00000061455 E012 42.3819184 0.0006363068 1.891344e-02 4.914206e-02 5 123170766 123171108 343 + 1.573 1.694 0.410
ENSG00000061455 E013 0.0000000       5 123173401 123173483 83 +      
ENSG00000061455 E014 39.1364729 0.0005912636 2.298465e-03 8.145714e-03 5 123180147 123180323 177 + 1.514 1.677 0.557
ENSG00000061455 E015 43.7902455 0.0037630495 1.533110e-04 7.617758e-04 5 123187087 123187332 246 + 1.536 1.749 0.726
ENSG00000061455 E016 70.1751094 0.0005047382 1.114632e-15 4.180630e-14 5 123187333 123187924 592 + 1.672 1.990 1.072
ENSG00000061455 E017 13.3131204 0.0029943749 5.712605e-01 6.975822e-01 5 123187925 123188180 256 + 1.179 1.126 -0.190
ENSG00000061455 E018 126.2535391 0.0003218346 3.234660e-38 9.947996e-36 5 123188181 123194266 6086 + 2.244 1.872 -1.245