ENSG00000060491

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000290291 ENSG00000060491 HEK293_OSMI2_2hA HEK293_TMG_2hB OGFR protein_coding protein_coding 43.74492 76.72363 27.38453 5.731318 1.077434 -1.485971 27.735613 53.912143 15.018549 2.0714953 0.5518727 -1.8431721 0.60359583 0.70790000 0.54860000 -0.15930000 3.630298e-02 2.395231e-09 FALSE TRUE
ENST00000370461 ENSG00000060491 HEK293_OSMI2_2hA HEK293_TMG_2hB OGFR protein_coding protein_coding 43.74492 76.72363 27.38453 5.731318 1.077434 -1.485971 1.718034 1.214935 1.718282 0.1653446 0.1532883 0.4966328 0.05402917 0.01566667 0.06323333 0.04756667 2.395231e-09 2.395231e-09 FALSE TRUE
MSTRG.20967.2 ENSG00000060491 HEK293_OSMI2_2hA HEK293_TMG_2hB OGFR protein_coding   43.74492 76.72363 27.38453 5.731318 1.077434 -1.485971 3.214027 4.499741 2.247637 1.2265895 0.3080812 -0.9982311 0.07662917 0.05776667 0.08323333 0.02546667 5.566563e-01 2.395231e-09 FALSE TRUE
MSTRG.20967.3 ENSG00000060491 HEK293_OSMI2_2hA HEK293_TMG_2hB OGFR protein_coding   43.74492 76.72363 27.38453 5.731318 1.077434 -1.485971 9.692654 15.583985 6.999230 2.8204774 0.8186240 -1.1536618 0.22313750 0.19976667 0.25400000 0.05423333 4.927160e-01 2.395231e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000060491 E001 0.3634088 0.4185398284 7.821668e-01   20 62804341 62804499 159 + 0.001 0.146 7.784
ENSG00000060491 E002 5.7758329 0.0340832688 8.580072e-01 9.123697e-01 20 62804796 62804812 17 + 0.723 0.765 0.170
ENSG00000060491 E003 9.4084285 0.0365912075 7.352102e-02 1.491544e-01 20 62804813 62804818 6 + 1.106 0.873 -0.862
ENSG00000060491 E004 14.6389604 0.0211803714 1.329834e-01 2.382594e-01 20 62804819 62804823 5 + 1.232 1.074 -0.565
ENSG00000060491 E005 29.7812699 0.0354106091 2.416241e-02 6.014316e-02 20 62804824 62804834 11 + 1.572 1.348 -0.773
ENSG00000060491 E006 32.2275925 0.0261875915 1.114735e-02 3.151942e-02 20 62804835 62804836 2 + 1.608 1.378 -0.792
ENSG00000060491 E007 327.8602489 0.0060087385 3.425939e-05 2.008336e-04 20 62804837 62805030 194 + 2.555 2.401 -0.514
ENSG00000060491 E008 55.4835003 0.0171359657 2.600748e-12 5.971444e-11 20 62805886 62807272 1387 + 2.016 1.479 -1.818
ENSG00000060491 E009 16.8538290 0.0730369712 2.864428e-03 9.865957e-03 20 62807273 62807536 264 + 1.459 1.032 -1.509
ENSG00000060491 E010 156.4562181 0.0024974464 3.714760e-02 8.576171e-02 20 62807537 62807541 5 + 2.172 2.102 -0.235
ENSG00000060491 E011 310.7850931 0.0004151876 1.640678e-06 1.300205e-05 20 62807542 62807605 64 + 2.493 2.390 -0.344
ENSG00000060491 E012 40.8153222 0.0005342797 3.605728e-23 3.255931e-21 20 62807606 62808246 641 + 1.890 1.354 -1.827
ENSG00000060491 E013 406.1393898 0.0001356885 1.098284e-07 1.107503e-06 20 62808247 62808325 79 + 2.603 2.509 -0.314
ENSG00000060491 E014 454.8457703 0.0001811882 1.086661e-01 2.035664e-01 20 62809585 62809663 79 + 2.598 2.576 -0.072
ENSG00000060491 E015 394.3794495 0.0001394627 4.893230e-01 6.267716e-01 20 62810499 62810565 67 + 2.501 2.524 0.076
ENSG00000060491 E016 9.5587134 0.0032402280 2.787633e-03 9.637197e-03 20 62811363 62811461 99 + 1.174 0.854 -1.180
ENSG00000060491 E017 508.2429746 0.0008351444 5.467335e-03 1.720149e-02 20 62811462 62811610 149 + 2.575 2.647 0.239
ENSG00000060491 E018 1549.2954946 0.0006864068 1.453559e-17 6.985602e-16 20 62812230 62813182 953 + 3.018 3.142 0.415
ENSG00000060491 E019 221.3759324 0.0028197242 5.583317e-01 6.865962e-01 20 62813183 62813362 180 + 2.279 2.269 -0.033
ENSG00000060491 E020 571.5705098 0.0029185745 7.805343e-08 8.094475e-07 20 62813363 62814000 638 + 2.543 2.719 0.588