ENSG00000058668

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000356729 ENSG00000058668 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP2B4 protein_coding protein_coding 8.680617 7.208594 8.7928 0.3306037 0.1610706 0.2862446 0.6888857 1.0731848 0.6465167 0.2213242 0.01812009 -0.7223758 0.08115000 0.1471333 0.07353333 -0.07360000 6.460785e-02 2.244812e-19 FALSE FALSE
ENST00000357681 ENSG00000058668 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP2B4 protein_coding protein_coding 8.680617 7.208594 8.7928 0.3306037 0.1610706 0.2862446 0.4201713 0.4403479 0.1639558 0.4403479 0.08216776 -1.3723192 0.05014167 0.0653000 0.01890000 -0.04640000 8.120640e-01 2.244812e-19 FALSE TRUE
ENST00000484746 ENSG00000058668 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP2B4 protein_coding nonsense_mediated_decay 8.680617 7.208594 8.7928 0.3306037 0.1610706 0.2862446 1.3646518 2.9975920 0.0000000 0.3435109 0.00000000 -8.2324651 0.17180417 0.4214333 0.00000000 -0.42143333 2.244812e-19 2.244812e-19 TRUE FALSE
MSTRG.2874.1 ENSG00000058668 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP2B4 protein_coding   8.680617 7.208594 8.7928 0.3306037 0.1610706 0.2862446 4.6014692 1.9861164 6.2965414 1.0460862 0.79811315 1.6596531 0.51617083 0.2649000 0.71473333 0.44983333 3.397511e-01 2.244812e-19 FALSE TRUE
MSTRG.2874.5 ENSG00000058668 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP2B4 protein_coding   8.680617 7.208594 8.7928 0.3306037 0.1610706 0.2862446 0.6886424 0.2960467 0.6397659 0.2960467 0.31374643 1.0861682 0.07576250 0.0439000 0.07283333 0.02893333 4.187506e-01 2.244812e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000058668 E001 0.1515154 0.0426761737 8.224420e-01   1 203626780 203626786 7 + 0.084 0.000 -13.329
ENSG00000058668 E002 0.7448917 0.1052090281 8.398641e-02 1.657877e-01 1 203626787 203626812 26 + 0.315 0.000 -16.933
ENSG00000058668 E003 0.5891098 0.0184338192 1.163974e-01 2.148187e-01 1 203626813 203626831 19 + 0.268 0.000 -16.585
ENSG00000058668 E004 2.1392434 0.1886260058 4.259269e-01 5.687962e-01 1 203626832 203627004 173 + 0.495 0.406 -0.463
ENSG00000058668 E005 2.4766279 0.0177409447 7.840893e-01 8.614982e-01 1 203627005 203627047 43 + 0.465 0.601 0.639
ENSG00000058668 E006 3.3601471 0.0046132815 8.846524e-01 9.302602e-01 1 203627048 203627096 49 + 0.576 0.697 0.520
ENSG00000058668 E007 4.9362309 0.0034730323 9.025793e-01 9.419639e-01 1 203627097 203627219 123 + 0.721 0.811 0.359
ENSG00000058668 E008 5.6191193 0.0332657999 3.265003e-01 4.699369e-01 1 203682742 203682808 67 + 0.815 0.776 -0.154
ENSG00000058668 E009 77.4361024 0.0003864189 6.178594e-08 6.537370e-07 1 203682809 203683398 590 + 1.904 1.797 -0.360
ENSG00000058668 E010 48.2754774 0.0005064630 9.492257e-10 1.413186e-08 1 203698157 203698354 198 + 1.733 1.516 -0.739
ENSG00000058668 E011 62.6473897 0.0004932007 1.377356e-06 1.109894e-05 1 203699460 203699717 258 + 1.814 1.709 -0.354
ENSG00000058668 E012 44.1457817 0.0005072453 5.365028e-06 3.791587e-05 1 203700206 203700331 126 + 1.674 1.541 -0.453
ENSG00000058668 E013 49.5375502 0.0004430754 9.697189e-06 6.467535e-05 1 203700798 203700923 126 + 1.716 1.605 -0.376
ENSG00000058668 E014 31.3540160 0.0006750164 1.707538e-03 6.305684e-03 1 203702044 203702079 36 + 1.513 1.429 -0.288
ENSG00000058668 E015 47.0804400 0.0090633744 4.656066e-02 1.030492e-01 1 203703652 203703813 162 + 1.664 1.651 -0.046
ENSG00000058668 E016 41.7889894 0.0022281767 6.400133e-02 1.333531e-01 1 203707009 203707223 215 + 1.602 1.616 0.046
ENSG00000058668 E017 63.7630052 0.0034537267 4.471256e-06 3.219301e-05 1 203707862 203708104 243 + 1.827 1.701 -0.425
ENSG00000058668 E018 63.7825613 0.0064774699 1.629972e-03 6.057027e-03 1 203709301 203709542 242 + 1.811 1.745 -0.221
ENSG00000058668 E019 73.7299578 0.0016573425 1.219430e-06 9.941362e-06 1 203710877 203711108 232 + 1.883 1.779 -0.351
ENSG00000058668 E020 60.4937772 0.0007020044 4.760103e-07 4.227392e-06 1 203711960 203712139 180 + 1.805 1.683 -0.414
ENSG00000058668 E021 40.9700741 0.0005459342 5.058198e-04 2.182080e-03 1 203713165 203713252 88 + 1.625 1.548 -0.263
ENSG00000058668 E022 46.0909878 0.0005239966 3.422438e-02 8.019042e-02 1 203714171 203714277 107 + 1.648 1.660 0.040
ENSG00000058668 E023 63.9677187 0.0004296430 6.342248e-02 1.323974e-01 1 203720549 203720740 192 + 1.775 1.817 0.140
ENSG00000058668 E024 64.4756804 0.0007632617 1.586449e-01 2.733274e-01 1 203721197 203721410 214 + 1.769 1.830 0.206
ENSG00000058668 E025 62.5796420 0.0004321651 2.009654e-03 7.256151e-03 1 203722478 203722689 212 + 1.789 1.772 -0.059
ENSG00000058668 E026 28.9294381 0.0225650180 9.006516e-03 2.629553e-02 1 203723881 203723896 16 + 1.500 1.364 -0.468
ENSG00000058668 E027 37.6860534 0.0033059055 1.242002e-01 2.259744e-01 1 203723897 203723960 64 + 1.561 1.587 0.091
ENSG00000058668 E028 21.3749062 0.0010059650 2.845398e-01 4.247917e-01 1 203723961 203723962 2 + 1.319 1.359 0.141
ENSG00000058668 E029 30.2380142 0.0041881902 6.725014e-01 7.784495e-01 1 203723963 203723988 26 + 1.441 1.535 0.320
ENSG00000058668 E030 44.1343207 0.0069624402 5.990590e-01 7.205390e-01 1 203727395 203727467 73 + 1.571 1.727 0.532
ENSG00000058668 E031 54.3319565 0.0005427649 5.098134e-01 6.450969e-01 1 203727468 203727571 104 + 1.686 1.779 0.315
ENSG00000058668 E032 0.9587465 0.0352331545 1.494483e-01 2.609209e-01 1 203727572 203727754 183 + 0.154 0.474 2.216
ENSG00000058668 E033 0.3332198 0.0283425951 6.876441e-01   1 203728151 203728310 160 + 0.084 0.175 1.221
ENSG00000058668 E034 11.9198910 0.0034478302 1.092003e-05 7.191209e-05 1 203733223 203733400 178 + 0.830 1.377 1.987
ENSG00000058668 E035 6.9244292 0.0030221013 6.940734e-06 4.784796e-05 1 203733401 203733632 232 + 0.551 1.201 2.541
ENSG00000058668 E036 43.2571201 0.0005521886 2.944415e-01 4.355411e-01 1 203739546 203739644 99 + 1.597 1.665 0.229
ENSG00000058668 E037 48.8581035 0.0011897476 6.177515e-04 2.598614e-03 1 203739645 203739764 120 + 1.694 1.631 -0.212
ENSG00000058668 E038 818.0089296 0.0111756466 4.944171e-12 1.082216e-10 1 203739765 203744081 4317 + 2.735 3.089 1.177