ENSG00000057757

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000246151 ENSG00000057757 HEK293_OSMI2_2hA HEK293_TMG_2hB PITHD1 protein_coding protein_coding 82.48255 126.4724 56.0053 14.07973 0.3628312 -1.175044 56.437000 78.549805 43.423998 7.370113 1.4137305 -0.8549666 0.6944917 0.63043333 0.7751000 0.1446667 0.171511805 1.250494e-09 FALSE  
ENST00000374524 ENSG00000057757 HEK293_OSMI2_2hA HEK293_TMG_2hB PITHD1 protein_coding protein_coding 82.48255 126.4724 56.0053 14.07973 0.3628312 -1.175044 15.722642 40.110585 1.582277 13.056381 0.7995985 -4.6551792 0.1663208 0.30710000 0.0282000 -0.2789000 0.114255815 1.250494e-09    
MSTRG.545.1 ENSG00000057757 HEK293_OSMI2_2hA HEK293_TMG_2hB PITHD1 protein_coding   82.48255 126.4724 56.0053 14.07973 0.3628312 -1.175044 5.810225 4.489202 6.895372 1.372386 0.8361832 0.6180500 0.0752125 0.03596667 0.1232667 0.0873000 0.002629638 1.250494e-09 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000057757 E001 6.347418 0.0125255909 2.267507e-03 8.052418e-03 1 23778387 23778402 16 + 1.037 0.684 -1.368
ENSG00000057757 E002 13.154138 0.0606611460 7.142768e-03 2.159450e-02 1 23778403 23778417 15 + 1.318 0.977 -1.223
ENSG00000057757 E003 363.304409 0.0085137855 2.471329e-05 1.501241e-04 1 23778418 23778713 296 + 2.617 2.470 -0.490
ENSG00000057757 E004 10.940186 0.0153730663 1.436400e-05 9.201379e-05 1 23779283 23779356 74 + 1.298 0.863 -1.585
ENSG00000057757 E005 10.937885 0.0022588421 7.761931e-09 9.766052e-08 1 23779357 23779437 81 + 1.337 0.821 -1.885
ENSG00000057757 E006 265.391367 0.0004155353 3.341713e-04 1.517007e-03 1 23779438 23779481 44 + 2.406 2.375 -0.101
ENSG00000057757 E007 14.271925 0.0012778854 1.630536e-18 8.748236e-17 1 23779482 23779863 382 + 1.519 0.790 -2.632
ENSG00000057757 E008 419.723638 0.0011117007 1.032098e-05 6.840468e-05 1 23779864 23779941 78 + 2.616 2.568 -0.159
ENSG00000057757 E009 2.518348 0.0057555617 5.291386e-07 4.657733e-06 1 23785192 23785311 120 + 0.907 0.161 -3.977
ENSG00000057757 E010 573.854774 0.0013194663 2.605349e-03 9.080257e-03 1 23785675 23785779 105 + 2.726 2.713 -0.042
ENSG00000057757 E011 632.713984 0.0004855612 1.123227e-01 2.089413e-01 1 23786315 23786423 109 + 2.699 2.783 0.281
ENSG00000057757 E012 16.166499 0.0456384929 1.571542e-01 2.713143e-01 1 23787258 23787274 17 + 1.288 1.161 -0.446
ENSG00000057757 E013 2870.786659 0.0040959658 1.314478e-06 1.064023e-05 1 23787275 23788232 958 + 3.316 3.446 0.432