ENSG00000057663

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369076 ENSG00000057663 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG5 protein_coding protein_coding 16.48625 5.992665 27.69102 0.5875894 0.3454111 2.206264 1.297456 0.2834257 3.302123 0.2834257 0.6400583 3.496689 0.06510833 0.0569000 0.1193333 0.06243333 2.594232e-01 2.792419e-13 FALSE TRUE
ENST00000613993 ENSG00000057663 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG5 protein_coding protein_coding 16.48625 5.992665 27.69102 0.5875894 0.3454111 2.206264 1.659859 0.0000000 3.239462 0.0000000 0.3726716 8.344057 0.07037917 0.0000000 0.1169000 0.11690000 2.792419e-13 2.792419e-13 TRUE TRUE
ENST00000635758 ENSG00000057663 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG5 protein_coding protein_coding 16.48625 5.992665 27.69102 0.5875894 0.3454111 2.206264 3.224541 1.4990642 4.534353 0.4060135 0.1740992 1.590421 0.21595000 0.2417667 0.1636333 -0.07813333 3.105338e-01 2.792419e-13 FALSE TRUE
ENST00000646025 ENSG00000057663 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG5 protein_coding nonsense_mediated_decay 16.48625 5.992665 27.69102 0.5875894 0.3454111 2.206264 6.525589 3.0424752 10.122973 0.3313428 0.4090263 1.731006 0.43160833 0.5074667 0.3654333 -0.14203333 2.183580e-03 2.792419e-13 TRUE TRUE
MSTRG.28708.9 ENSG00000057663 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG5 protein_coding   16.48625 5.992665 27.69102 0.5875894 0.3454111 2.206264 3.524583 0.9906702 6.209736 0.3411064 0.3272060 2.635887 0.19625000 0.1678000 0.2245667 0.05676667 6.181177e-01 2.792419e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000057663 E001 0.0000000       6 106045423 106046570 1148 -      
ENSG00000057663 E002 1.5283913 0.1294008093 4.094353e-01 5.530478e-01 6 106046571 106046825 255 - 0.410 0.207 -1.360
ENSG00000057663 E003 0.0000000       6 106072083 106072211 129 -      
ENSG00000057663 E004 0.1451727 0.0433931844 1.000000e+00   6 106078255 106078315 61 - 0.070 0.000 -7.391
ENSG00000057663 E005 110.6865044 0.0007355672 5.510353e-01 6.805611e-01 6 106184476 106185941 1466 - 1.988 2.012 0.080
ENSG00000057663 E006 121.9025359 0.0013680182 2.682299e-05 1.614416e-04 6 106185942 106186227 286 - 1.989 2.145 0.522
ENSG00000057663 E007 198.7635689 0.0001894906 5.344453e-08 5.724814e-07 6 106186228 106186462 235 - 2.205 2.348 0.477
ENSG00000057663 E008 120.2087992 0.0003051103 5.291942e-02 1.143346e-01 6 106186463 106186539 77 - 2.010 2.079 0.232
ENSG00000057663 E009 158.3620817 0.0002676157 6.322283e-01 7.471105e-01 6 106186540 106186676 137 - 2.151 2.136 -0.049
ENSG00000057663 E010 0.6558305 0.1670605103 2.571472e-01 3.941947e-01 6 106201746 106201971 226 - 0.129 0.347 1.820
ENSG00000057663 E011 135.2491874 0.0003115001 4.615402e-01 6.014859e-01 6 106201972 106202089 118 - 2.086 2.061 -0.085
ENSG00000057663 E012 0.1472490 0.0435554998 1.000000e+00   6 106202090 106202429 340 - 0.070 0.000 -7.391
ENSG00000057663 E013 0.0000000       6 106217464 106217601 138 -      
ENSG00000057663 E014 122.4092762 0.0002478733 3.517642e-01 4.959911e-01 6 106248150 106248244 95 - 2.044 2.010 -0.114
ENSG00000057663 E015 152.6628318 0.0008433774 3.242442e-01 4.676098e-01 6 106279661 106279823 163 - 2.138 2.102 -0.121
ENSG00000057663 E016 108.3634523 0.0002961899 1.853981e-03 6.770328e-03 6 106293028 106293106 79 - 2.011 1.884 -0.426
ENSG00000057663 E017 114.6549564 0.0003953748 4.202346e-04 1.851963e-03 6 106308364 106308491 128 - 2.037 1.895 -0.477
ENSG00000057663 E018 136.2225480 0.0002412428 8.096004e-03 2.402399e-02 6 106316101 106316266 166 - 2.103 2.009 -0.315
ENSG00000057663 E019 1.8174891 0.0080097239 3.850460e-01 5.292065e-01 6 106325276 106325525 250 - 0.378 0.536 0.810
ENSG00000057663 E020 76.2354581 0.0075923104 6.184464e-02 1.297283e-01 6 106325526 106325791 266 - 1.860 1.744 -0.394