ENSG00000056277

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370978 ENSG00000056277 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF280C protein_coding protein_coding 5.013006 2.477227 7.589492 0.1734007 0.1977409 1.611365 4.3139232 2.477227 6.361952 0.1734007 0.26061683 1.357197 0.90599583 1 0.8376 -0.1624 5.899399e-10 5.899399e-10 FALSE TRUE
ENST00000447817 ENSG00000056277 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF280C protein_coding protein_coding 5.013006 2.477227 7.589492 0.1734007 0.1977409 1.611365 0.6990826 0.000000 1.227540 0.0000000 0.06588554 6.951332 0.09400417 0 0.1624 0.1624 6.297244e-10 5.899399e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000056277 E001 278.53554 0.0300196730 2.041115e-06 1.584832e-05 X 130202707 130204992 2286 - 2.229 2.660 1.439
ENSG00000056277 E002 26.92738 0.0304236454 8.829235e-01 9.290816e-01 X 130205117 130205153 37 - 1.330 1.482 0.524
ENSG00000056277 E003 38.52265 0.0210935973 9.847317e-01 9.945118e-01 X 130205297 130205415 119 - 1.481 1.627 0.499
ENSG00000056277 E004 29.61556 0.0139757998 3.264382e-01 4.698824e-01 X 130209653 130209715 63 - 1.395 1.450 0.191
ENSG00000056277 E005 37.84245 0.0011667298 1.616019e-01 2.771892e-01 X 130215193 130215333 141 - 1.500 1.555 0.188
ENSG00000056277 E006 58.18475 0.0034036330 6.064356e-02 1.277151e-01 X 130215791 130216101 311 - 1.687 1.725 0.127
ENSG00000056277 E007 39.27879 0.0007320151 7.377054e-03 2.219760e-02 X 130220349 130220480 132 - 1.534 1.504 -0.102
ENSG00000056277 E008 32.72972 0.0014256492 2.870566e-02 6.941616e-02 X 130226759 130226905 147 - 1.458 1.446 -0.040
ENSG00000056277 E009 24.01909 0.0040548780 1.339695e-01 2.396680e-01 X 130227682 130227782 101 - 1.325 1.342 0.060
ENSG00000056277 E010 27.14596 0.0007346455 4.138343e-02 9.359702e-02 X 130228977 130229134 158 - 1.381 1.368 -0.044
ENSG00000056277 E011 37.96282 0.0013410407 2.266077e-03 8.049002e-03 X 130230510 130230727 218 - 1.530 1.466 -0.217
ENSG00000056277 E012 28.53475 0.0007291990 3.591050e-03 1.198474e-02 X 130236214 130236320 107 - 1.412 1.330 -0.283
ENSG00000056277 E013 33.50963 0.0005998606 1.529256e-04 7.600425e-04 X 130236469 130236639 171 - 1.489 1.356 -0.458
ENSG00000056277 E014 25.14330 0.0008956133 2.801631e-04 1.299069e-03 X 130239582 130239693 112 - 1.375 1.207 -0.587
ENSG00000056277 E015 25.87653 0.0008753828 5.898495e-03 1.834415e-02 X 130243563 130243699 137 - 1.369 1.288 -0.282
ENSG00000056277 E016 20.68970 0.0009897041 3.305868e-02 7.792544e-02 X 130243800 130243865 66 - 1.272 1.225 -0.167
ENSG00000056277 E017 38.18449 0.0009610648 7.737947e-05 4.156311e-04 X 130246859 130247005 147 - 1.544 1.415 -0.443
ENSG00000056277 E018 33.23970 0.0073460745 7.445852e-03 2.237582e-02 X 130260419 130260465 47 - 1.473 1.394 -0.270
ENSG00000056277 E019 35.50163 0.0006866344 5.155039e-04 2.217393e-03 X 130268762 130268899 138 - 1.504 1.404 -0.346