ENSG00000055732

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000341115 ENSG00000055732 HEK293_OSMI2_2hA HEK293_TMG_2hB MCOLN3 protein_coding protein_coding 5.152586 1.844162 9.134092 0.08736483 0.1778132 2.302072 0.4156215 0.03545042 1.0157087 0.03545042 0.53321564 4.4961835 0.05601250 0.02030000 0.1115333 0.09123333 6.548766e-01 2.080767e-05 FALSE TRUE
ENST00000370587 ENSG00000055732 HEK293_OSMI2_2hA HEK293_TMG_2hB MCOLN3 protein_coding protein_coding 5.152586 1.844162 9.134092 0.08736483 0.1778132 2.302072 0.4258829 0.10468661 0.3559024 0.03087569 0.08094166 1.6737619 0.08706250 0.05796667 0.0389000 -0.01906667 7.588342e-01 2.080767e-05 FALSE TRUE
ENST00000370589 ENSG00000055732 HEK293_OSMI2_2hA HEK293_TMG_2hB MCOLN3 protein_coding protein_coding 5.152586 1.844162 9.134092 0.08736483 0.1778132 2.302072 1.7897933 0.65927372 4.2259546 0.02932016 0.63519764 2.6620188 0.30670417 0.35793333 0.4615333 0.10360000 5.706374e-01 2.080767e-05 FALSE TRUE
ENST00000474447 ENSG00000055732 HEK293_OSMI2_2hA HEK293_TMG_2hB MCOLN3 protein_coding processed_transcript 5.152586 1.844162 9.134092 0.08736483 0.1778132 2.302072 1.4269266 0.89643034 1.4830801 0.09489626 0.23190694 0.7200235 0.34901667 0.48456667 0.1615000 -0.32306667 2.080767e-05 2.080767e-05 FALSE TRUE
ENST00000490600 ENSG00000055732 HEK293_OSMI2_2hA HEK293_TMG_2hB MCOLN3 protein_coding nonsense_mediated_decay 5.152586 1.844162 9.134092 0.08736483 0.1778132 2.302072 0.3572832 0.08450949 0.9978210 0.04730503 0.11012273 3.4146364 0.05987917 0.04420000 0.1093000 0.06510000 1.941976e-01 2.080767e-05 FALSE TRUE
MSTRG.1514.2 ENSG00000055732 HEK293_OSMI2_2hA HEK293_TMG_2hB MCOLN3 protein_coding   5.152586 1.844162 9.134092 0.08736483 0.1778132 2.302072 0.4176631 0.00000000 0.7339939 0.00000000 0.46621271 6.2172188 0.07858333 0.00000000 0.0822000 0.08220000 4.735075e-01 2.080767e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000055732 E001 0.9996463 0.0132094093 7.534717e-02 1.520742e-01 1 85018082 85018082 1 - 0.161 0.495 2.249
ENSG00000055732 E002 84.9016001 0.0018978061 1.515494e-09 2.172904e-08 1 85018083 85018907 825 - 1.772 2.044 0.915
ENSG00000055732 E003 9.0131599 0.0354275473 1.525225e-01 2.651106e-01 1 85018908 85018908 1 - 0.870 1.087 0.805
ENSG00000055732 E004 55.7372035 0.0083864574 7.563134e-03 2.267827e-02 1 85018909 85019257 349 - 1.618 1.805 0.634
ENSG00000055732 E005 35.8804623 0.0320107852 4.249301e-02 9.561458e-02 1 85021070 85021276 207 - 1.414 1.649 0.803
ENSG00000055732 E006 32.3744534 0.0288567383 5.278843e-01 6.608391e-01 1 85022070 85022192 123 - 1.408 1.496 0.300
ENSG00000055732 E007 29.3713128 0.0013290863 6.764665e-01 7.814285e-01 1 85022299 85022400 102 - 1.380 1.424 0.153
ENSG00000055732 E008 35.5363396 0.0080462585 4.811440e-06 3.440044e-05 1 85022401 85023520 1120 - 1.375 1.718 1.174
ENSG00000055732 E009 2.3844488 0.0155255240 4.005346e-01 5.442258e-01 1 85024541 85024556 16 - 0.422 0.585 0.792
ENSG00000055732 E010 8.4845944 0.0020192964 9.377333e-01 9.648535e-01 1 85024557 85024823 267 - 0.887 0.908 0.079
ENSG00000055732 E011 25.5940081 0.0010340688 7.051656e-01 8.035821e-01 1 85024824 85025938 1115 - 1.319 1.362 0.149
ENSG00000055732 E012 27.4980929 0.0015921650 1.012578e-04 5.276721e-04 1 85025939 85026088 150 - 1.414 1.039 -1.327
ENSG00000055732 E013 2.8463819 0.0061386296 3.004698e-01 4.421624e-01 1 85026089 85026171 83 - 0.468 0.658 0.871
ENSG00000055732 E014 26.7415334 0.0026865514 9.502022e-02 1.829069e-01 1 85026172 85026284 113 - 1.377 1.238 -0.486
ENSG00000055732 E015 35.9706088 0.0188437986 5.297937e-03 1.674270e-02 1 85029106 85029205 100 - 1.520 1.240 -0.972
ENSG00000055732 E016 1.5532566 0.0087410922 4.961142e-01 6.328475e-01 1 85029206 85029959 754 - 0.370 0.233 -0.920
ENSG00000055732 E017 36.8059314 0.0006314916 7.142357e-05 3.870869e-04 1 85032696 85032792 97 - 1.535 1.220 -1.093
ENSG00000055732 E018 36.9314185 0.0005749985 3.081625e-03 1.050594e-02 1 85032872 85032956 85 - 1.524 1.304 -0.758
ENSG00000055732 E019 34.9641266 0.0042214108 2.620860e-05 1.581255e-04 1 85034098 85034251 154 - 1.518 1.139 -1.321
ENSG00000055732 E020 3.2674945 0.0056901907 6.449027e-03 1.979053e-02 1 85036710 85036868 159 - 0.632 0.000 -11.927
ENSG00000055732 E021 25.1041757 0.0366194431 7.478526e-03 2.245727e-02 1 85041010 85041177 168 - 1.382 1.033 -1.239
ENSG00000055732 E022 32.0262632 0.0081393415 1.066231e-02 3.036275e-02 1 85045133 85045362 230 - 1.467 1.238 -0.798
ENSG00000055732 E023 1.6369186 0.0096803768 7.568647e-02 1.526046e-01 1 85047310 85047574 265 - 0.422 0.000 -10.927
ENSG00000055732 E024 4.0652503 0.0087788445 2.631519e-02 6.457937e-02 1 85047575 85047609 35 - 0.689 0.233 -2.451
ENSG00000055732 E025 13.9066232 0.0015610752 3.493906e-02 8.153810e-02 1 85048396 85048500 105 - 1.122 0.869 -0.938