ENSG00000054690

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000329153 ENSG00000054690 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH1 protein_coding protein_coding 7.005503 8.529803 4.716297 0.1183492 0.09683944 -0.8534924 1.8836641 1.62822732 2.0397226 0.22654223 0.04869884 0.3232931 0.31217500 0.191633333 0.43243333 0.24080000 5.106410e-05 1.426634e-08 FALSE TRUE
ENST00000559168 ENSG00000054690 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH1 protein_coding nonsense_mediated_decay 7.005503 8.529803 4.716297 0.1183492 0.09683944 -0.8534924 0.2982366 0.87306529 0.0000000 0.87306529 0.00000000 -6.4644482 0.03549583 0.099766667 0.00000000 -0.09976667 8.739782e-01 1.426634e-08   FALSE
ENST00000560100 ENSG00000054690 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH1 protein_coding retained_intron 7.005503 8.529803 4.716297 0.1183492 0.09683944 -0.8534924 0.2646235 0.03027197 0.3872878 0.03027197 0.13496851 3.3023367 0.04272083 0.003566667 0.08230000 0.07873333 1.908947e-02 1.426634e-08 FALSE FALSE
ENST00000561456 ENSG00000054690 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH1 protein_coding retained_intron 7.005503 8.529803 4.716297 0.1183492 0.09683944 -0.8534924 0.4157371 0.32041128 0.2527441 0.19714442 0.12824958 -0.3306044 0.05729167 0.038133333 0.05266667 0.01453333 9.422482e-01 1.426634e-08 FALSE FALSE
MSTRG.9755.1 ENSG00000054690 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH1 protein_coding   7.005503 8.529803 4.716297 0.1183492 0.09683944 -0.8534924 0.4320519 0.38855855 0.5512146 0.14337147 0.10932260 0.4937608 0.06922083 0.046000000 0.11636667 0.07036667 2.299610e-01 1.426634e-08 FALSE TRUE
MSTRG.9755.12 ENSG00000054690 HEK293_OSMI2_2hA HEK293_TMG_2hB PLEKHH1 protein_coding   7.005503 8.529803 4.716297 0.1183492 0.09683944 -0.8534924 2.2045245 4.25523170 0.6166484 0.40622128 0.01652763 -2.7668959 0.27075000 0.499500000 0.13096667 -0.36853333 1.426634e-08 1.426634e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000054690 E001 4.4901803 0.0057858932 4.202613e-04 1.852037e-03 14 67533055 67533289 235 + 0.934 0.485 -1.887
ENSG00000054690 E002 6.3766687 0.0026573660 8.542022e-05 4.536373e-04 14 67533290 67533398 109 + 1.060 0.626 -1.702
ENSG00000054690 E003 3.2601170 0.0051096992 2.500770e-04 1.175533e-03 14 67541834 67541851 18 + 0.854 0.337 -2.392
ENSG00000054690 E004 10.6557608 0.0048399531 1.920923e-08 2.247626e-07 14 67541852 67541993 142 + 1.297 0.755 -2.004
ENSG00000054690 E005 11.4610409 0.0015245291 6.133847e-07 5.333634e-06 14 67555825 67555887 63 + 1.289 0.854 -1.584
ENSG00000054690 E006 17.9834889 0.0115999527 1.007807e-03 3.985348e-03 14 67557269 67557418 150 + 1.396 1.145 -0.880
ENSG00000054690 E007 17.1417483 0.0011283487 1.145466e-02 3.224492e-02 14 67559608 67559691 84 + 1.327 1.178 -0.526
ENSG00000054690 E008 15.6727329 0.0018207678 3.852420e-02 8.831644e-02 14 67561954 67562035 82 + 1.281 1.161 -0.424
ENSG00000054690 E009 39.3685640 0.0026684311 2.103640e-04 1.008268e-03 14 67562137 67562694 558 + 1.685 1.525 -0.542
ENSG00000054690 E010 23.8894202 0.0130659387 1.355650e-01 2.419033e-01 14 67562695 67562894 200 + 1.426 1.357 -0.240
ENSG00000054690 E011 1.3191365 0.0130192300 2.371017e-01 3.708295e-01 14 67569033 67569137 105 + 0.448 0.274 -1.040
ENSG00000054690 E012 10.6671287 0.0474179408 2.113369e-01 3.400701e-01 14 67569138 67569216 79 + 1.112 1.010 -0.372
ENSG00000054690 E013 3.5646615 0.0048905380 1.304621e-01 2.348013e-01 14 67569739 67569920 182 + 0.448 0.732 1.282
ENSG00000054690 E014 15.4330533 0.0062605639 2.612546e-02 6.419692e-02 14 67569921 67570012 92 + 1.281 1.135 -0.517
ENSG00000054690 E015 2.3229486 0.1692536424 3.268164e-01 4.702701e-01 14 67570468 67571062 595 + 0.591 0.445 -0.699
ENSG00000054690 E016 5.0930303 0.0057223713 1.150337e-02 3.235751e-02 14 67571063 67571751 689 + 0.916 0.626 -1.165
ENSG00000054690 E017 12.9056630 0.0118057206 1.639665e-03 6.088389e-03 14 67571752 67571787 36 + 1.264 0.994 -0.971
ENSG00000054690 E018 19.0835015 0.0159219090 4.854403e-05 2.744083e-04 14 67571788 67571902 115 + 1.455 1.118 -1.181
ENSG00000054690 E019 0.5922303 0.0179825655 1.773984e-01 2.978684e-01 14 67571903 67572134 232 + 0.319 0.111 -1.891
ENSG00000054690 E020 20.2831672 0.0203886856 1.261353e-02 3.499956e-02 14 67572135 67572277 143 + 1.415 1.224 -0.665
ENSG00000054690 E021 19.0044010 0.0010259660 1.273648e-02 3.529162e-02 14 67573276 67573386 111 + 1.363 1.226 -0.480
ENSG00000054690 E022 19.8313826 0.0015590004 2.291027e-01 3.613590e-01 14 67573801 67573887 87 + 1.320 1.283 -0.130
ENSG00000054690 E023 23.7526512 0.0011987580 7.529069e-02 1.519942e-01 14 67574242 67574403 162 + 1.414 1.345 -0.241
ENSG00000054690 E024 18.2534083 0.0025764832 2.071348e-02 5.298337e-02 14 67575392 67575472 81 + 1.342 1.211 -0.460
ENSG00000054690 E025 2.2068178 0.0068420160 1.705015e-01 2.888061e-01 14 67575473 67575599 127 + 0.319 0.594 1.432
ENSG00000054690 E026 24.6551772 0.0096248054 1.171106e-01 2.158249e-01 14 67575823 67576005 183 + 1.438 1.368 -0.243
ENSG00000054690 E027 19.5819913 0.0010663008 1.613353e-01 2.768196e-01 14 67576395 67576503 109 + 1.327 1.276 -0.180
ENSG00000054690 E028 22.7580800 0.0011877263 1.803570e-01 3.016199e-01 14 67577302 67577414 113 + 1.389 1.350 -0.137
ENSG00000054690 E029 26.2883748 0.0008676341 2.243274e-01 3.556033e-01 14 67578023 67578199 177 + 1.438 1.413 -0.085
ENSG00000054690 E030 13.4316687 0.0015807188 1.255853e-01 2.279821e-01 14 67578534 67578562 29 + 1.189 1.105 -0.303
ENSG00000054690 E031 18.2425182 0.0277096468 1.783536e-01 2.990773e-01 14 67578563 67578631 69 + 1.312 1.235 -0.273
ENSG00000054690 E032 25.3576224 0.0123790409 7.219165e-01 8.161415e-01 14 67579134 67579293 160 + 1.389 1.421 0.111
ENSG00000054690 E033 13.3567380 0.0336748539 7.419363e-01 8.309642e-01 14 67579294 67579311 18 + 1.086 1.187 0.362
ENSG00000054690 E034 0.3729606 0.0292212713 8.003505e-01 8.727415e-01 14 67579429 67579429 1 + 0.134 0.112 -0.302
ENSG00000054690 E035 1.7296479 0.0209199044 2.532452e-01 3.897382e-01 14 67579430 67579519 90 + 0.501 0.338 -0.883
ENSG00000054690 E036 1.5426475 0.0119994718 1.499180e-03 5.632874e-03 14 67579520 67579720 201 + 0.629 0.112 -3.473
ENSG00000054690 E037 26.8375025 0.0086903607 1.620453e-01 2.777737e-01 14 67579721 67579876 156 + 1.465 1.414 -0.176
ENSG00000054690 E038 3.0991172 0.3005300991 8.487978e-01 9.061288e-01 14 67579877 67580307 431 + 0.449 0.659 0.975
ENSG00000054690 E039 0.3268771 0.0290169437 7.994473e-01   14 67580895 67580937 43 + 0.134 0.112 -0.304
ENSG00000054690 E040 21.3796846 0.0027468910 9.845950e-02 1.881580e-01 14 67580938 67581001 64 + 1.383 1.314 -0.239
ENSG00000054690 E041 22.8521828 0.0009140845 1.500412e-01 2.617318e-01 14 67581002 67581038 37 + 1.396 1.350 -0.159
ENSG00000054690 E042 0.8438645 0.0142795010 9.505139e-01 9.730256e-01 14 67581586 67582068 483 + 0.236 0.274 0.281
ENSG00000054690 E043 35.2499915 0.0006800763 8.320631e-01 8.947459e-01 14 67582069 67582210 142 + 1.516 1.562 0.156
ENSG00000054690 E044 34.1068908 0.0006498317 3.377247e-02 7.929815e-02 14 67583741 67583883 143 + 1.408 1.598 0.653
ENSG00000054690 E045 29.3230448 0.0006779122 8.812674e-01 9.278964e-01 14 67583995 67584110 116 + 1.426 1.494 0.233
ENSG00000054690 E046 16.2496552 0.0011087293 7.025945e-01 8.014042e-01 14 67584111 67584124 14 + 1.209 1.233 0.085
ENSG00000054690 E047 0.9222279 0.0136968632 6.730847e-01 7.788708e-01 14 67585116 67585567 452 + 0.319 0.274 -0.307
ENSG00000054690 E048 29.5372194 0.0007152524 6.281808e-01 7.437716e-01 14 67585568 67585654 87 + 1.455 1.481 0.092
ENSG00000054690 E049 19.6921536 0.0011114859 1.872234e-01 3.102463e-01 14 67585951 67585981 31 + 1.327 1.283 -0.157
ENSG00000054690 E050 18.9164978 0.0009844409 6.601852e-02 1.367305e-01 14 67585982 67585990 9 + 1.335 1.248 -0.304
ENSG00000054690 E051 20.6773788 0.0009037147 8.192133e-02 1.625735e-01 14 67585991 67586015 25 + 1.363 1.289 -0.257
ENSG00000054690 E052 24.0058928 0.0021153282 3.539498e-01 4.982223e-01 14 67586016 67586097 82 + 1.383 1.373 -0.034
ENSG00000054690 E053 0.5149242 0.2093263727 8.320150e-01 8.947158e-01 14 67586368 67586410 43 + 0.134 0.201 0.699
ENSG00000054690 E054 0.3332198 0.0291545471 7.990302e-01   14 67586411 67586456 46 + 0.134 0.112 -0.304
ENSG00000054690 E055 0.9599055 0.0172375139 6.734877e-01 7.791627e-01 14 67586457 67586809 353 + 0.319 0.274 -0.306
ENSG00000054690 E056 0.4449813 0.0229454106 3.839005e-01 5.281110e-01 14 67586810 67586847 38 + 0.236 0.111 -1.307
ENSG00000054690 E057 1.1802934 0.0111960458 4.078787e-01 5.514697e-01 14 67586848 67586949 102 + 0.389 0.274 -0.718
ENSG00000054690 E058 49.2091381 0.0011913904 9.464722e-01 9.703942e-01 14 67587074 67587300 227 + 1.644 1.707 0.214
ENSG00000054690 E059 272.4526259 0.0032942720 1.522259e-11 3.076789e-10 14 67587301 67588793 1493 + 2.243 2.516 0.911
ENSG00000054690 E060 194.0348284 0.0179313605 6.303142e-10 9.712961e-09 14 67588794 67589612 819 + 1.947 2.415 1.564