ENSG00000053900

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000315368 ENSG00000053900 HEK293_OSMI2_2hA HEK293_TMG_2hB ANAPC4 protein_coding protein_coding 11.28223 4.600302 16.84852 0.5316888 0.4780714 1.870545 6.896023 3.1907744 10.279243 0.21144345 0.3842372 1.684644 0.59875000 0.71113333 0.6103667 -0.10076667 0.637770467 0.002241434 FALSE TRUE
ENST00000505991 ENSG00000053900 HEK293_OSMI2_2hA HEK293_TMG_2hB ANAPC4 protein_coding protein_coding 11.28223 4.600302 16.84852 0.5316888 0.4780714 1.870545 1.394745 0.2569169 2.910340 0.08584635 0.2110193 3.451674 0.10973750 0.05426667 0.1734000 0.11913333 0.002241434 0.002241434 FALSE FALSE
ENST00000510092 ENSG00000053900 HEK293_OSMI2_2hA HEK293_TMG_2hB ANAPC4 protein_coding protein_coding 11.28223 4.600302 16.84852 0.5316888 0.4780714 1.870545 1.017801 0.4907226 1.852223 0.32362591 0.3081666 1.894943 0.08798333 0.09843333 0.1100000 0.01156667 0.788341518 0.002241434 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000053900 E001 0.1515154 0.0432858055 1.000000e+00   4 25375746 25375776 31 + 0.072 0.000 -9.059
ENSG00000053900 E002 6.3591667 0.0026378630 7.256178e-01 8.188453e-01 4 25377263 25377292 30 + 0.830 0.799 -0.125
ENSG00000053900 E003 14.3486923 0.0012644010 7.429278e-01 8.317296e-01 4 25377293 25377314 22 + 1.140 1.125 -0.052
ENSG00000053900 E004 17.1458320 0.0010537060 4.037198e-01 5.474996e-01 4 25377315 25377319 5 + 1.222 1.162 -0.214
ENSG00000053900 E005 27.0155971 0.0007356362 3.678233e-02 8.505295e-02 4 25377320 25377344 25 + 1.431 1.284 -0.510
ENSG00000053900 E006 5.7821506 0.0031805664 6.394480e-01 7.528433e-01 4 25377345 25377356 12 + 0.807 0.756 -0.202
ENSG00000053900 E007 10.0817591 0.0016555062 7.069300e-02 1.444956e-01 4 25377357 25377417 61 + 1.045 0.837 -0.780
ENSG00000053900 E008 51.5604570 0.0004744715 1.248299e-03 4.804685e-03 4 25377418 25377556 139 + 1.708 1.538 -0.581
ENSG00000053900 E009 38.2500164 0.0005806346 8.234755e-03 2.437882e-02 4 25380374 25380445 72 + 1.579 1.419 -0.548
ENSG00000053900 E010 27.2964066 0.0006978818 1.483021e-02 4.010818e-02 4 25380446 25380479 34 + 1.445 1.270 -0.607
ENSG00000053900 E011 0.5180316 0.0203267463 7.114838e-03 2.152281e-02 4 25380480 25380608 129 + 0.000 0.441 13.765
ENSG00000053900 E012 48.7140686 0.0006726120 1.107824e-01 2.066908e-01 4 25383261 25383393 133 + 1.667 1.593 -0.250
ENSG00000053900 E013 38.2467589 0.0005993968 9.922785e-01 9.992621e-01 4 25388500 25388574 75 + 1.540 1.559 0.065
ENSG00000053900 E014 33.6042450 0.0006089643 9.708829e-01 9.858174e-01 4 25388717 25388743 27 + 1.485 1.507 0.075
ENSG00000053900 E015 33.7502438 0.0006516650 3.349271e-01 4.786693e-01 4 25388838 25388882 45 + 1.469 1.552 0.283
ENSG00000053900 E016 40.3508833 0.0005241911 7.532952e-01 8.392901e-01 4 25390136 25390220 85 + 1.567 1.566 -0.001
ENSG00000053900 E017 43.9089507 0.0005536889 2.643215e-01 4.022800e-01 4 25390911 25391015 105 + 1.615 1.566 -0.165
ENSG00000053900 E018 46.6970615 0.0004614542 2.394579e-03 8.443535e-03 4 25392338 25392421 84 + 1.667 1.499 -0.572
ENSG00000053900 E019 40.4192074 0.0005563553 1.398066e-05 8.980416e-05 4 25393805 25393860 56 + 1.628 1.345 -0.971
ENSG00000053900 E020 38.3078892 0.0005785581 2.174232e-05 1.336389e-04 4 25393861 25393891 31 + 1.605 1.322 -0.976
ENSG00000053900 E021 49.0462413 0.0005558005 2.490063e-05 1.511003e-04 4 25394310 25394374 65 + 1.701 1.457 -0.833
ENSG00000053900 E022 36.9743030 0.0013000522 3.590532e-02 8.339960e-02 4 25394671 25394713 43 + 1.556 1.429 -0.434
ENSG00000053900 E023 0.9704895 0.0650061746 6.774378e-01 7.821611e-01 4 25394758 25394828 71 + 0.279 0.201 -0.612
ENSG00000053900 E024 46.9212910 0.0018657169 1.498485e-01 2.614799e-01 4 25394829 25394905 77 + 1.644 1.574 -0.240
ENSG00000053900 E025 1.8090580 0.0077848324 5.245536e-01 6.580391e-01 4 25394906 25395420 515 + 0.448 0.337 -0.617
ENSG00000053900 E026 46.3396021 0.0047445409 2.322783e-01 3.651471e-01 4 25396664 25396718 55 + 1.637 1.575 -0.212
ENSG00000053900 E027 38.8511824 0.0064428398 3.608762e-01 5.052576e-01 4 25396719 25396764 46 + 1.560 1.509 -0.175
ENSG00000053900 E028 32.5477544 0.0006692754 7.466046e-01 8.343073e-01 4 25396848 25396899 52 + 1.477 1.474 -0.011
ENSG00000053900 E029 38.4495445 0.0005539856 8.532080e-01 9.091240e-01 4 25402971 25403026 56 + 1.545 1.552 0.025
ENSG00000053900 E030 34.7637101 0.0005934948 7.609145e-01 8.448773e-01 4 25405573 25405600 28 + 1.500 1.499 -0.003
ENSG00000053900 E031 32.7684789 0.0006457397 9.734391e-01 9.874066e-01 4 25405601 25405619 19 + 1.469 1.491 0.074
ENSG00000053900 E032 4.7190153 0.0212149727 8.174127e-01 8.845535e-01 4 25405620 25405622 3 + 0.726 0.708 -0.076
ENSG00000053900 E033 45.8968832 0.0006065262 8.893238e-01 9.332609e-01 4 25406829 25406885 57 + 1.609 1.637 0.094
ENSG00000053900 E034 53.5956613 0.0023731914 2.270174e-01 3.588436e-01 4 25407197 25407253 57 + 1.699 1.648 -0.174
ENSG00000053900 E035 74.6114897 0.0033354640 2.422342e-01 3.768271e-01 4 25409698 25409791 94 + 1.835 1.794 -0.138
ENSG00000053900 E036 2.3521729 0.0119397827 6.038495e-03 1.871166e-02 4 25412918 25413644 727 + 0.589 0.000 -13.045
ENSG00000053900 E037 72.7821212 0.0004265252 8.958866e-01 9.374669e-01 4 25413645 25413742 98 + 1.807 1.833 0.087
ENSG00000053900 E038 5.7821626 0.0063888698 7.827412e-01 8.605221e-01 4 25413743 25414323 581 + 0.781 0.837 0.218
ENSG00000053900 E039 39.8747072 0.0008015038 3.680775e-01 5.124768e-01 4 25414324 25414385 62 + 1.538 1.612 0.254
ENSG00000053900 E040 2.4179501 0.1396223908 1.308520e-01 2.353331e-01 4 25414386 25414465 80 + 0.567 0.202 -2.182
ENSG00000053900 E041 25.6571283 0.0146333631 9.596607e-02 1.843339e-01 4 25414466 25414504 39 + 1.318 1.487 0.583
ENSG00000053900 E042 43.6568252 0.0092419244 1.763966e-02 4.636018e-02 4 25414599 25414700 102 + 1.540 1.723 0.624
ENSG00000053900 E043 9.3987544 0.0018371288 8.606813e-01 9.141409e-01 4 25415152 25415465 314 + 0.968 0.965 -0.011
ENSG00000053900 E044 55.1180188 0.0004822895 4.425168e-03 1.433247e-02 4 25415466 25415540 75 + 1.646 1.809 0.554
ENSG00000053900 E045 9.7251494 0.0020636155 3.571130e-01 5.015307e-01 4 25415541 25416424 884 + 0.942 1.064 0.450
ENSG00000053900 E046 81.1235178 0.0003561584 4.616922e-06 3.312988e-05 4 25416425 25416598 174 + 1.796 2.004 0.701
ENSG00000053900 E047 7.4726759 0.0108255426 4.659483e-01 6.055368e-01 4 25416599 25417615 1017 + 0.853 0.964 0.422
ENSG00000053900 E048 91.2949974 0.0028244975 1.057360e-08 1.297368e-07 4 25417616 25417739 124 + 1.825 2.099 0.923
ENSG00000053900 E049 1.2168251 0.0110405580 6.685907e-02 1.381527e-01 4 25417740 25418154 415 + 0.188 0.525 2.122
ENSG00000053900 E050 99.3087719 0.0259615155 1.548243e-03 5.792063e-03 4 25418155 25418480 326 + 1.853 2.146 0.982
ENSG00000053900 E051 0.8814298 0.0478343308 6.430870e-01 7.557045e-01 4 25418481 25418498 18 + 0.236 0.336 0.698
ENSG00000053900 E052 0.0000000       4 25425317 25425395 79 +