ENSG00000053372

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000330263 ENSG00000053372 HEK293_OSMI2_2hA HEK293_TMG_2hB MRTO4 protein_coding protein_coding 137.1655 214.4958 119.0389 15.98321 1.797095 -0.8494628 101.3653 165.42459 82.78535 14.450157 2.2346874 -0.9986393 0.7297250 0.7697000 0.6952667 -0.07443333 0.01343204 0.01343204 FALSE  
MSTRG.466.1 ENSG00000053372 HEK293_OSMI2_2hA HEK293_TMG_2hB MRTO4 protein_coding   137.1655 214.4958 119.0389 15.98321 1.797095 -0.8494628 29.9810 43.24549 29.60212 2.080615 0.2215708 -0.5466956 0.2231417 0.2032667 0.2488333 0.04556667 0.16832747 0.01343204 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000053372 E001 385.940263 5.511014e-03 6.788059e-01 7.831838e-01 1 19251572 19251804 233 + 2.558 2.539 -0.062
ENSG00000053372 E002 424.297382 2.272368e-03 5.652665e-01 6.923826e-01 1 19251805 19251818 14 + 2.598 2.582 -0.054
ENSG00000053372 E003 767.739091 5.958005e-04 5.025829e-06 3.576203e-05 1 19251819 19251834 16 + 2.898 2.822 -0.254
ENSG00000053372 E004 1030.811586 7.928709e-04 5.948805e-08 6.311864e-07 1 19251835 19251863 29 + 3.036 2.945 -0.302
ENSG00000053372 E005 15.548734 1.314498e-02 4.031668e-01 5.469611e-01 1 19251864 19252073 210 + 1.238 1.158 -0.285
ENSG00000053372 E006 31.795538 7.701627e-04 4.338306e-01 5.762315e-01 1 19252074 19252248 175 + 1.507 1.462 -0.155
ENSG00000053372 E007 1287.945893 5.596700e-04 1.788277e-09 2.528803e-08 1 19254782 19254840 59 + 3.129 3.043 -0.286
ENSG00000053372 E008 1751.151198 2.724584e-04 3.408840e-08 3.799015e-07 1 19255948 19256051 104 + 3.244 3.186 -0.194
ENSG00000053372 E009 1.784075 1.896710e-01 7.934285e-01 8.679269e-01 1 19256052 19256052 1 + 0.457 0.409 -0.255
ENSG00000053372 E010 1455.611551 1.399119e-04 4.855379e-01 6.234113e-01 1 19257064 19257145 82 + 3.126 3.123 -0.009
ENSG00000053372 E011 1349.505710 8.092741e-05 2.490304e-01 3.847721e-01 1 19257454 19257521 68 + 3.079 3.098 0.062
ENSG00000053372 E012 18.444267 1.713915e-02 1.962262e-07 1.883110e-06 1 19257712 19257832 121 + 1.543 1.025 -1.823
ENSG00000053372 E013 1746.839387 4.167777e-04 1.510267e-03 5.669375e-03 1 19257833 19257984 152 + 3.171 3.216 0.150
ENSG00000053372 E014 1289.428584 6.201434e-04 1.799618e-01 3.011397e-01 1 19258477 19258553 77 + 3.053 3.079 0.085
ENSG00000053372 E015 666.769408 1.366242e-04 3.261744e-01 4.696175e-01 1 19258681 19258683 3 + 2.772 2.794 0.073
ENSG00000053372 E016 2239.149982 2.346591e-04 2.064849e-04 9.917842e-04 1 19258684 19259047 364 + 3.280 3.323 0.140
ENSG00000053372 E017 3112.468649 5.455649e-04 8.883531e-11 1.581133e-09 1 19259048 19260128 1081 + 3.395 3.473 0.259
ENSG00000053372 E018 3.986014 3.265415e+00 8.171625e-01 8.843701e-01 1 19299221 19299612 392 + 0.001 0.780 11.909