ENSG00000050748

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000393360 ENSG00000050748 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK9 protein_coding protein_coding 29.94725 17.79731 44.10461 0.4915926 1.119939 1.308787 5.0424261 4.607308 6.5072318 1.0808462 0.76900305 0.4972074 0.18946667 0.25726667 0.14786667 -0.109400000 2.307692e-01 2.000977e-08 FALSE TRUE
ENST00000425491 ENSG00000050748 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK9 protein_coding protein_coding 29.94725 17.79731 44.10461 0.4915926 1.119939 1.308787 1.6673630 1.221950 2.4628365 0.2238700 0.07840018 1.0052228 0.05662917 0.06810000 0.05586667 -0.012233333 6.281812e-01 2.000977e-08 FALSE TRUE
ENST00000523135 ENSG00000050748 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK9 protein_coding processed_transcript 29.94725 17.79731 44.10461 0.4915926 1.119939 1.308787 0.6397602 1.258562 0.5778123 0.1591003 0.14913014 -1.1097665 0.03084583 0.07123333 0.01330000 -0.057933333 4.402431e-05 2.000977e-08 FALSE TRUE
MSTRG.27451.10 ENSG00000050748 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK9 protein_coding   29.94725 17.79731 44.10461 0.4915926 1.119939 1.308787 3.3308532 0.000000 4.8759779 0.0000000 1.54805884 8.9325035 0.09249167 0.00000000 0.11050000 0.110500000 2.000977e-08 2.000977e-08 FALSE TRUE
MSTRG.27451.15 ENSG00000050748 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK9 protein_coding   29.94725 17.79731 44.10461 0.4915926 1.119939 1.308787 1.8398374 0.000000 2.4260072 0.0000000 1.22834437 7.9283746 0.04799583 0.00000000 0.05623333 0.056233333 2.756707e-01 2.000977e-08 FALSE TRUE
MSTRG.27451.16 ENSG00000050748 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK9 protein_coding   29.94725 17.79731 44.10461 0.4915926 1.119939 1.308787 7.7778827 5.143419 13.7605371 0.4263671 1.97884674 1.4179829 0.24797500 0.28846667 0.31006667 0.021600000 8.985035e-01 2.000977e-08 FALSE TRUE
MSTRG.27451.5 ENSG00000050748 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK9 protein_coding   29.94725 17.79731 44.10461 0.4915926 1.119939 1.308787 2.0954557 1.447087 3.2162177 0.4319970 0.12909195 1.1467567 0.07499583 0.08243333 0.07286667 -0.009566667 1.000000e+00 2.000977e-08 FALSE TRUE
MSTRG.27451.8 ENSG00000050748 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK9 protein_coding   29.94725 17.79731 44.10461 0.4915926 1.119939 1.308787 2.7074328 1.352954 4.4904251 0.2188003 0.32316491 1.7233247 0.08592917 0.07680000 0.10170000 0.024900000 4.744608e-01 2.000977e-08 FALSE TRUE
MSTRG.27451.9 ENSG00000050748 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK9 protein_coding   29.94725 17.79731 44.10461 0.4915926 1.119939 1.308787 2.6181694 2.030928 3.3549324 0.3185878 0.48016565 0.7213521 0.10095833 0.11510000 0.07663333 -0.038466667 4.742550e-01 2.000977e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000050748 E001 0.1515154 0.0434139153 1.000000e+00   5 180233143 180233593 451 - 0.091 0.000 -10.974
ENSG00000050748 E002 61.6251300 0.0255713992 4.019653e-04 1.782043e-03 5 180233594 180233879 286 - 1.624 1.970 1.170
ENSG00000050748 E003 181.8612095 0.0132200670 6.010423e-05 3.319699e-04 5 180233880 180234480 601 - 2.132 2.397 0.883
ENSG00000050748 E004 192.7026996 0.0074029853 8.901242e-02 1.736176e-01 5 180234481 180235304 824 - 2.237 2.336 0.329
ENSG00000050748 E005 284.6429689 0.0001814073 1.986170e-02 5.119809e-02 5 180235305 180236007 703 - 2.422 2.482 0.200
ENSG00000050748 E006 188.7883398 0.0002207646 4.103392e-05 2.359401e-04 5 180236008 180236125 118 - 2.220 2.338 0.393
ENSG00000050748 E007 107.4339564 0.0002885748 9.584096e-05 5.025125e-04 5 180236126 180236136 11 - 1.966 2.111 0.486
ENSG00000050748 E008 116.6243977 0.0002614665 7.771052e-03 2.320415e-02 5 180236137 180236153 17 - 2.021 2.120 0.331
ENSG00000050748 E009 134.8168709 0.0002524785 2.157433e-02 5.479766e-02 5 180236154 180236177 24 - 2.090 2.171 0.273
ENSG00000050748 E010 128.3868029 0.0002510492 8.516014e-03 2.507785e-02 5 180236178 180236186 9 - 2.062 2.156 0.314
ENSG00000050748 E011 416.5606024 0.0010067307 7.440347e-02 1.505788e-01 5 180236187 180236525 339 - 2.590 2.637 0.159
ENSG00000050748 E012 107.0677601 0.0003181736 4.521282e-01 5.930152e-01 5 180236526 180236526 1 - 2.007 2.043 0.123
ENSG00000050748 E013 23.8558620 0.0021276423 7.694665e-01 8.511139e-01 5 180236527 180236531 5 - 1.376 1.408 0.112
ENSG00000050748 E014 19.2471697 0.0399651714 4.756939e-01 6.143861e-01 5 180236532 180238308 1777 - 1.323 1.241 -0.288
ENSG00000050748 E015 0.2924217 0.0272601979 4.506401e-01   5 180238309 180238331 23 - 0.166 0.000 -11.966
ENSG00000050748 E016 207.1620970 0.0036515996 3.214153e-01 4.646584e-01 5 180238332 180238403 72 - 2.316 2.286 -0.099
ENSG00000050748 E017 214.3183165 0.0048765348 6.212596e-02 1.302175e-01 5 180239924 180239987 64 - 2.346 2.275 -0.236
ENSG00000050748 E018 214.6778365 0.0013505646 6.930072e-02 1.422037e-01 5 180241031 180241155 125 - 2.338 2.292 -0.153
ENSG00000050748 E019 67.1779133 0.0008289600 1.754092e-01 2.951982e-01 5 180242573 180242577 5 - 1.840 1.787 -0.181
ENSG00000050748 E020 238.1575489 0.0014328423 1.801653e-03 6.604319e-03 5 180242578 180242727 150 - 2.396 2.311 -0.284
ENSG00000050748 E021 134.5927769 0.0006644699 3.166457e-02 7.519596e-02 5 180242728 180242755 28 - 2.143 2.079 -0.212
ENSG00000050748 E022 5.0585618 0.0045601459 8.238617e-01 8.890250e-01 5 180242756 180242900 145 - 0.769 0.746 -0.095
ENSG00000050748 E023 21.1009904 0.0009828577 5.655662e-02 1.206898e-01 5 180246028 180246544 517 - 1.271 1.424 0.533
ENSG00000050748 E024 76.5456683 0.0166297593 9.140744e-01 9.495743e-01 5 180246545 180247438 894 - 1.875 1.889 0.049
ENSG00000050748 E025 211.2608201 0.0019100654 3.140350e-05 1.859214e-04 5 180247439 180247510 72 - 2.360 2.231 -0.431
ENSG00000050748 E026 3.8650590 0.2001531504 3.279495e-01 4.714247e-01 5 180247663 180247728 66 - 0.754 0.515 -1.037
ENSG00000050748 E027 2.8701002 0.0855474089 4.801256e-01 6.184320e-01 5 180247855 180247926 72 - 0.521 0.666 0.648
ENSG00000050748 E028 293.7883627 0.0001693127 2.189943e-09 3.046094e-08 5 180248973 180249138 166 - 2.500 2.378 -0.406
ENSG00000050748 E029 6.6428691 0.0079720853 2.544871e-03 8.897467e-03 5 180253507 180253677 171 - 0.674 1.061 1.500
ENSG00000050748 E030 194.7366005 0.0014226298 7.979922e-02 1.592462e-01 5 180261684 180261822 139 - 2.297 2.250 -0.159
ENSG00000050748 E031 0.4772466 0.0206427689 2.591305e-01 3.964974e-01 5 180262957 180263089 133 - 0.091 0.281 1.973
ENSG00000050748 E032 102.8367566 0.0058742977 7.969981e-01 8.704787e-01 5 180264781 180264839 59 - 2.008 1.998 -0.032
ENSG00000050748 E033 0.0000000       5 180264840 180264842 3 -      
ENSG00000050748 E034 157.4441002 0.0002719124 1.921756e-02 4.980898e-02 5 180269280 180269409 130 - 2.212 2.152 -0.200
ENSG00000050748 E035 19.2049730 0.0010592208 2.469057e-01 3.823093e-01 5 180279796 180280036 241 - 1.326 1.241 -0.299
ENSG00000050748 E036 130.9517645 0.0002864759 6.059169e-06 4.233262e-05 5 180280440 180280608 169 - 2.159 2.019 -0.469
ENSG00000050748 E037 0.1451727 0.0430827773 1.000000e+00   5 180281962 180282035 74 - 0.091 0.000 -10.966
ENSG00000050748 E038 1.3640767 0.0103458386 1.135992e-01 2.107838e-01 5 180282494 180282543 50 - 0.456 0.163 -2.026
ENSG00000050748 E039 18.4221083 0.0217838787 7.726668e-03 2.309260e-02 5 180286975 180287067 93 - 1.363 1.064 -1.059
ENSG00000050748 E040 0.2214452 0.0415974200 2.538314e-01   5 180291469 180291524 56 - 0.000 0.164 12.643
ENSG00000050748 E041 33.8128915 0.0140807009 6.041082e-06 4.222339e-05 5 180291848 180292099 252 - 1.645 1.240 -1.400
ENSG00000050748 E042 0.1817044 0.0417146750 2.542691e-01   5 180292871 180295256 2386 - 0.000 0.163 12.640