ENSG00000050426

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262055 ENSG00000050426 HEK293_OSMI2_2hA HEK293_TMG_2hB LETMD1 protein_coding protein_coding 40.11231 40.13229 35.73519 3.686126 0.7332724 -0.167374 9.134526 8.1379378 8.1646710 0.69194472 0.53760079 0.004725712 0.23154167 0.204433333 0.22903333 0.02460000 7.458008e-01 2.777137e-21 FALSE  
ENST00000547318 ENSG00000050426 HEK293_OSMI2_2hA HEK293_TMG_2hB LETMD1 protein_coding nonsense_mediated_decay 40.11231 40.13229 35.73519 3.686126 0.7332724 -0.167374 2.410931 2.7539137 2.0216425 0.50474501 0.08208131 -0.444066002 0.06115417 0.069866667 0.05653333 -0.01333333 7.823690e-01 2.777137e-21 FALSE  
ENST00000547877 ENSG00000050426 HEK293_OSMI2_2hA HEK293_TMG_2hB LETMD1 protein_coding retained_intron 40.11231 40.13229 35.73519 3.686126 0.7332724 -0.167374 2.600564 1.3526922 2.8773035 0.11407888 0.28469046 1.083263094 0.06445833 0.034300000 0.08043333 0.04613333 5.347554e-04 2.777137e-21    
ENST00000548390 ENSG00000050426 HEK293_OSMI2_2hA HEK293_TMG_2hB LETMD1 protein_coding retained_intron 40.11231 40.13229 35.73519 3.686126 0.7332724 -0.167374 1.446183 0.1539708 2.5552474 0.01535294 0.38712502 3.967587349 0.03597917 0.003866667 0.07116667 0.06730000 7.306786e-17 2.777137e-21 FALSE  
ENST00000549340 ENSG00000050426 HEK293_OSMI2_2hA HEK293_TMG_2hB LETMD1 protein_coding protein_coding 40.11231 40.13229 35.73519 3.686126 0.7332724 -0.167374 1.381553 0.0000000 2.0135190 0.00000000 0.23118569 7.660722561 0.03509583 0.000000000 0.05656667 0.05656667 2.777137e-21 2.777137e-21 FALSE  
ENST00000550100 ENSG00000050426 HEK293_OSMI2_2hA HEK293_TMG_2hB LETMD1 protein_coding nonsense_mediated_decay 40.11231 40.13229 35.73519 3.686126 0.7332724 -0.167374 3.236872 4.4620418 1.0853185 0.83504912 0.85711440 -2.029583198 0.08100417 0.109466667 0.02970000 -0.07976667 2.609655e-01 2.777137e-21 TRUE  
ENST00000553043 ENSG00000050426 HEK293_OSMI2_2hA HEK293_TMG_2hB LETMD1 protein_coding protein_coding 40.11231 40.13229 35.73519 3.686126 0.7332724 -0.167374 1.832824 3.0290152 0.7853051 1.65662607 0.45051177 -1.934023568 0.04472083 0.074133333 0.02153333 -0.05260000 8.340485e-01 2.777137e-21    
ENST00000623495 ENSG00000050426 HEK293_OSMI2_2hA HEK293_TMG_2hB LETMD1 protein_coding TEC 40.11231 40.13229 35.73519 3.686126 0.7332724 -0.167374 2.330968 1.4287762 2.3401685 0.17648137 0.26667708 0.707922008 0.05632917 0.035766667 0.06543333 0.02966667 2.602451e-02 2.777137e-21    
MSTRG.7300.10 ENSG00000050426 HEK293_OSMI2_2hA HEK293_TMG_2hB LETMD1 protein_coding   40.11231 40.13229 35.73519 3.686126 0.7332724 -0.167374 2.462201 4.9833645 0.8308238 0.49928110 0.83082382 -2.570136770 0.06199167 0.124366667 0.02286667 -0.10150000 8.297986e-02 2.777137e-21 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000050426 E001 1.429663 0.0183812408 5.361526e-01 6.679062e-01 12 51047962 51048298 337 + 0.313 0.447 0.769
ENSG00000050426 E002 1.471582 0.0099818149 4.891493e-01 6.266339e-01 12 51048301 51048312 12 + 0.441 0.343 -0.549
ENSG00000050426 E003 1.471582 0.0099818149 4.891493e-01 6.266339e-01 12 51048313 51048316 4 + 0.441 0.343 -0.549
ENSG00000050426 E004 1.653287 0.0155575437 7.072616e-01 8.052151e-01 12 51048317 51048317 1 + 0.441 0.399 -0.226
ENSG00000050426 E005 1.653287 0.0155575437 7.072616e-01 8.052151e-01 12 51048318 51048318 1 + 0.441 0.399 -0.226
ENSG00000050426 E006 1.653287 0.0155575437 7.072616e-01 8.052151e-01 12 51048319 51048319 1 + 0.441 0.399 -0.226
ENSG00000050426 E007 1.800536 0.0537095258 5.294706e-01 6.622429e-01 12 51048320 51048320 1 + 0.493 0.399 -0.487
ENSG00000050426 E008 1.948829 0.0182011845 6.814399e-01 7.851263e-01 12 51048321 51048326 6 + 0.493 0.448 -0.228
ENSG00000050426 E009 2.389646 0.0090152563 8.249917e-02 1.634531e-01 12 51048327 51048328 2 + 0.655 0.399 -1.227
ENSG00000050426 E010 5.010313 0.0034249464 5.594617e-02 1.196041e-01 12 51048329 51048337 9 + 0.886 0.664 -0.890
ENSG00000050426 E011 10.781757 0.0145027113 3.574142e-01 5.018272e-01 12 51048338 51048340 3 + 1.101 1.028 -0.265
ENSG00000050426 E012 11.852380 0.0063432879 1.659080e-01 2.828696e-01 12 51048341 51048342 2 + 1.158 1.051 -0.385
ENSG00000050426 E013 13.029567 0.0023900499 3.188132e-02 7.562347e-02 12 51048343 51048343 1 + 1.226 1.062 -0.589
ENSG00000050426 E014 24.789575 0.0144831225 1.022424e-04 5.323633e-04 12 51048344 51048345 2 + 1.559 1.240 -1.104
ENSG00000050426 E015 45.465354 0.0013126289 1.142066e-03 4.444558e-03 12 51048346 51048357 12 + 1.737 1.600 -0.464
ENSG00000050426 E016 64.074283 0.0008847915 3.904208e-02 8.927464e-02 12 51048358 51048371 14 + 1.839 1.782 -0.190
ENSG00000050426 E017 118.927315 0.0022740898 1.578553e-01 2.722688e-01 12 51048372 51048478 107 + 2.084 2.064 -0.068
ENSG00000050426 E018 11.234710 0.0391022578 4.992435e-02 1.090387e-01 12 51048479 51048518 40 + 1.199 0.964 -0.854
ENSG00000050426 E019 39.028393 0.0115241158 2.145698e-06 1.657611e-05 12 51048519 51048873 355 + 1.756 1.426 -1.127
ENSG00000050426 E020 23.613071 0.0008016811 5.013022e-06 3.567786e-05 12 51048874 51049012 139 + 1.528 1.244 -0.986
ENSG00000050426 E021 9.304231 0.0018155712 2.788306e-03 9.639170e-03 12 51049013 51049033 21 + 1.158 0.881 -1.017
ENSG00000050426 E022 109.933131 0.0103317780 5.679918e-01 6.947101e-01 12 51049034 51049077 44 + 2.041 2.039 -0.007
ENSG00000050426 E023 166.278402 0.0003159612 3.250173e-01 4.684502e-01 12 51049078 51049185 108 + 2.214 2.225 0.036
ENSG00000050426 E024 16.348400 0.0011540836 3.713034e-23 3.348039e-21 12 51049186 51049430 245 + 1.546 0.634 -3.368
ENSG00000050426 E025 10.115335 0.0042111819 1.012728e-10 1.785393e-09 12 51052088 51052091 4 + 1.316 0.634 -2.578
ENSG00000050426 E026 101.487917 0.0002907483 2.496363e-06 1.902367e-05 12 51052092 51052207 116 + 2.077 1.951 -0.421
ENSG00000050426 E027 8.788120 0.0088068369 6.105412e-11 1.114523e-09 12 51052208 51052246 39 + 1.278 0.492 -3.094
ENSG00000050426 E028 12.304489 0.0014067440 7.035684e-12 1.503033e-10 12 51052247 51052361 115 + 1.385 0.741 -2.366
ENSG00000050426 E029 69.423028 0.0004437398 1.379163e-03 5.238755e-03 12 51053778 51053811 34 + 1.902 1.807 -0.322
ENSG00000050426 E030 92.155191 0.0004241962 3.751988e-01 5.196003e-01 12 51053812 51053860 49 + 1.963 1.968 0.018
ENSG00000050426 E031 8.887959 0.0197912586 3.724302e-04 1.667473e-03 12 51055699 51055834 136 + 1.189 0.783 -1.511
ENSG00000050426 E032 83.615490 0.0096608708 7.474099e-01 8.349339e-01 12 51055835 51055879 45 + 1.916 1.927 0.037
ENSG00000050426 E033 72.128536 0.0087244233 5.714879e-01 6.977699e-01 12 51055880 51055908 29 + 1.861 1.859 -0.005
ENSG00000050426 E034 106.935555 0.0003056579 6.955518e-01 7.961320e-01 12 51055909 51055986 78 + 1.998 2.050 0.174
ENSG00000050426 E035 103.050319 0.0002946652 1.457657e-01 2.558970e-01 12 51055987 51056021 35 + 1.958 2.048 0.301
ENSG00000050426 E036 10.527462 0.0050170455 1.622191e-04 8.015210e-04 12 51056022 51056056 35 + 1.227 0.865 -1.326
ENSG00000050426 E037 13.364155 0.0017316725 6.710252e-07 5.783455e-06 12 51056057 51056143 87 + 1.352 0.930 -1.515
ENSG00000050426 E038 80.638134 0.0003656718 7.812301e-01 8.594556e-01 12 51056144 51056149 6 + 1.891 1.918 0.091
ENSG00000050426 E039 101.466936 0.0002817994 9.002853e-01 9.403728e-01 12 51056150 51056178 29 + 1.981 2.024 0.143
ENSG00000050426 E040 146.490796 0.0002246319 9.432526e-01 9.684011e-01 12 51056179 51056245 67 + 2.144 2.181 0.122
ENSG00000050426 E041 16.175790 0.0061030036 6.474034e-08 6.821253e-07 12 51056246 51056349 104 + 1.438 0.974 -1.647
ENSG00000050426 E042 151.063640 0.0002529943 3.968188e-01 5.405865e-01 12 51056350 51056370 21 + 2.141 2.204 0.211
ENSG00000050426 E043 275.398925 0.0002456098 2.165016e-03 7.734994e-03 12 51056371 51056502 132 + 2.373 2.478 0.351
ENSG00000050426 E044 18.216531 0.0010239364 1.508302e-04 7.507509e-04 12 51056503 51056679 177 + 1.415 1.153 -0.918
ENSG00000050426 E045 17.540817 0.0049436136 6.607162e-04 2.755061e-03 12 51056680 51056952 273 + 1.397 1.143 -0.892
ENSG00000050426 E046 34.373525 0.0062944464 1.387216e-04 6.979627e-04 12 51057471 51058005 535 + 1.660 1.430 -0.789
ENSG00000050426 E047 10.211142 0.0115414498 2.598669e-02 6.392142e-02 12 51058006 51058031 26 + 1.158 0.945 -0.779
ENSG00000050426 E048 139.146991 0.0004366969 1.377341e-03 5.232738e-03 12 51058032 51058074 43 + 2.057 2.196 0.464
ENSG00000050426 E049 142.793554 0.0002250689 2.264316e-03 8.043846e-03 12 51058075 51058128 54 + 2.074 2.205 0.438
ENSG00000050426 E050 24.139191 0.0277275489 1.246466e-04 6.350390e-04 12 51058129 51058603 475 + 1.577 1.190 -1.342
ENSG00000050426 E051 10.234111 0.0016398629 1.598076e-05 1.013629e-04 12 51058604 51058738 135 + 1.235 0.827 -1.503
ENSG00000050426 E052 51.293956 0.0238127483 2.050359e-02 5.255221e-02 12 51058739 51059360 622 + 1.802 1.640 -0.549
ENSG00000050426 E053 939.738526 0.0036745563 6.897849e-14 2.005489e-12 12 51059361 51060424 1064 + 2.823 3.048 0.748