ENSG00000049541

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000055077 ENSG00000049541 HEK293_OSMI2_2hA HEK293_TMG_2hB RFC2 protein_coding protein_coding 93.76071 134.5121 81.45687 22.40105 0.81449 -0.7235582 39.978465 58.689016 34.407931 6.725467 2.4204681 -0.77017597 0.4289375 0.44550000 0.42193333 -0.02356667 8.553649e-01 3.023775e-11 FALSE TRUE
ENST00000352131 ENSG00000049541 HEK293_OSMI2_2hA HEK293_TMG_2hB RFC2 protein_coding protein_coding 93.76071 134.5121 81.45687 22.40105 0.81449 -0.7235582 5.861967 10.287519 3.963886 2.037227 0.6028411 -1.37367458 0.0614375 0.07706667 0.04853333 -0.02853333 2.329509e-01 3.023775e-11 FALSE TRUE
ENST00000485545 ENSG00000049541 HEK293_OSMI2_2hA HEK293_TMG_2hB RFC2 protein_coding protein_coding 93.76071 134.5121 81.45687 22.40105 0.81449 -0.7235582 18.091764 33.381187 8.646856 5.650007 0.7530627 -1.94755230 0.1787083 0.24813333 0.10636667 -0.14176667 3.043420e-10 3.023775e-11 FALSE TRUE
ENST00000491206 ENSG00000049541 HEK293_OSMI2_2hA HEK293_TMG_2hB RFC2 protein_coding nonsense_mediated_decay 93.76071 134.5121 81.45687 22.40105 0.81449 -0.7235582 13.705323 15.080113 14.582218 5.103165 1.1159101 -0.04840436 0.1487167 0.10446667 0.17896667 0.07450000 2.264211e-01 3.023775e-11 FALSE FALSE
ENST00000497430 ENSG00000049541 HEK293_OSMI2_2hA HEK293_TMG_2hB RFC2 protein_coding protein_coding 93.76071 134.5121 81.45687 22.40105 0.81449 -0.7235582 7.680078 7.432067 8.904765 1.997348 1.4073458 0.26049332 0.0887375 0.05526667 0.10960000 0.05433333 1.046216e-01 3.023775e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000049541 E001 10.4577322 1.673907e-03 9.340519e-01 9.624456e-01 7 74231499 74231501 3 - 1.027 1.029 0.010
ENSG00000049541 E002 10.3094390 1.723140e-03 9.963763e-01 1.000000e+00 7 74231502 74231503 2 - 1.027 1.020 -0.025
ENSG00000049541 E003 112.8290637 2.703781e-04 1.162000e-07 1.166503e-06 7 74231504 74231699 196 - 1.882 2.077 0.654
ENSG00000049541 E004 277.4461673 1.543731e-03 4.997132e-13 1.286965e-11 7 74231700 74231772 73 - 2.251 2.466 0.715
ENSG00000049541 E005 230.7116734 5.675805e-04 2.351874e-14 7.322565e-13 7 74231773 74231773 1 - 2.176 2.389 0.711
ENSG00000049541 E006 1540.6890607 6.526466e-05 3.248965e-40 1.144064e-37 7 74231774 74232103 330 - 3.066 3.191 0.416
ENSG00000049541 E007 1201.3147183 9.768830e-05 2.715991e-11 5.268650e-10 7 74232104 74232216 113 - 2.999 3.067 0.226
ENSG00000049541 E008 9.6192775 1.863047e-03 3.560735e-01 5.004609e-01 7 74233775 74233894 120 - 0.921 1.020 0.368
ENSG00000049541 E009 4.2654180 1.579290e-02 8.234095e-01 8.887193e-01 7 74235531 74235531 1 - 0.669 0.693 0.100
ENSG00000049541 E010 1241.2973819 2.398711e-04 6.520450e-01 7.627886e-01 7 74235532 74235645 114 - 3.064 3.059 -0.017
ENSG00000049541 E011 4.3252217 2.342127e-02 1.317453e-01 2.365513e-01 7 74237289 74237349 61 - 0.843 0.603 -0.987
ENSG00000049541 E012 3.1031972 2.107111e-02 6.970872e-01 7.973008e-01 7 74237350 74237361 12 - 0.624 0.550 -0.333
ENSG00000049541 E013 858.3164556 1.149951e-04 1.941314e-03 7.044441e-03 7 74237362 74237407 46 - 2.937 2.889 -0.160
ENSG00000049541 E014 807.0959162 6.139866e-04 2.836614e-01 4.238090e-01 7 74237408 74237442 35 - 2.894 2.866 -0.091
ENSG00000049541 E015 625.6587722 6.929102e-04 1.928778e-01 3.173897e-01 7 74238923 74238936 14 - 2.788 2.754 -0.113
ENSG00000049541 E016 723.1205808 6.091374e-04 2.113281e-01 3.400668e-01 7 74238937 74238988 52 - 2.849 2.818 -0.105
ENSG00000049541 E017 1197.3534455 2.743818e-04 1.465950e-01 2.570599e-01 7 74239938 74240095 158 - 3.066 3.038 -0.091
ENSG00000049541 E018 897.9561531 1.345703e-04 3.929815e-03 1.294565e-02 7 74243146 74243246 101 - 2.954 2.909 -0.151
ENSG00000049541 E019 530.1946956 4.886411e-04 4.538417e-05 2.583890e-04 7 74246662 74246714 53 - 2.752 2.667 -0.282
ENSG00000049541 E020 453.5834341 1.705475e-04 2.001466e-10 3.353839e-09 7 74246715 74246763 49 - 2.709 2.589 -0.399
ENSG00000049541 E021 498.5911104 5.305393e-04 2.271547e-03 8.064195e-03 7 74249012 74249118 107 - 2.714 2.643 -0.236
ENSG00000049541 E022 17.9284507 2.607018e-03 5.316235e-06 3.760248e-05 7 74249119 74249220 102 - 1.476 1.098 -1.325
ENSG00000049541 E023 396.1457063 4.603506e-03 2.301533e-01 3.626416e-01 7 74249739 74249780 42 - 2.609 2.539 -0.234
ENSG00000049541 E024 6.1905380 4.073020e-02 6.513179e-01 7.621997e-01 7 74249781 74249829 49 - 0.782 0.833 0.201
ENSG00000049541 E025 546.2816134 3.756542e-03 4.572944e-03 1.474480e-02 7 74252429 74252498 70 - 2.778 2.663 -0.384
ENSG00000049541 E026 0.1515154 4.395356e-02 2.592194e-01   7 74253599 74253717 119 - 0.164 0.000 -9.903
ENSG00000049541 E027 516.5853453 4.128060e-03 2.264393e-03 8.043922e-03 7 74254271 74255047 777 - 2.762 2.633 -0.430