ENSG00000049239

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000377403 ENSG00000049239 HEK293_OSMI2_2hA HEK293_TMG_2hB H6PD protein_coding protein_coding 5.466432 6.374238 4.965402 0.4972043 0.1700164 -0.3597015 5.002026 6.128467 4.028336 0.4148075 0.2084071 -0.6041176 0.9068542 0.9631333 0.8120333 -0.1511 0.001943923 0.001943923 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000049239 E001 0.884652 0.0135997507 2.753774e-02 6.708686e-02 1 9234774 9234788 15 + 0.459 0.108 -2.734
ENSG00000049239 E002 5.474730 0.0932535448 2.713357e-04 1.263022e-03 1 9234789 9234886 98 + 1.076 0.434 -2.667
ENSG00000049239 E003 7.664772 0.1130651759 5.680361e-03 1.776785e-02 1 9234887 9234989 103 + 1.138 0.684 -1.735
ENSG00000049239 E004 9.260812 0.0474961665 5.857359e-03 1.823718e-02 1 9234990 9235066 77 + 1.162 0.831 -1.229
ENSG00000049239 E005 2.742556 0.1431501113 8.398896e-01 9.000219e-01 1 9237131 9237217 87 + 0.511 0.587 0.351
ENSG00000049239 E006 2.086177 0.0153256556 3.347485e-02 7.872203e-02 1 9239844 9239914 71 + 0.642 0.328 -1.584
ENSG00000049239 E007 3.508641 0.0150723250 4.512924e-02 1.004335e-01 1 9239915 9240018 104 + 0.770 0.514 -1.109
ENSG00000049239 E008 4.427548 0.0036420257 2.284735e-02 5.742286e-02 1 9244925 9244932 8 + 0.868 0.613 -1.043
ENSG00000049239 E009 77.113780 0.0077364955 8.453813e-10 1.269258e-08 1 9244933 9245561 629 + 2.026 1.752 -0.924
ENSG00000049239 E010 34.658448 0.0010286446 4.228728e-05 2.423670e-04 1 9246966 9247083 118 + 1.638 1.461 -0.604
ENSG00000049239 E011 43.300501 0.0005659595 4.008876e-06 2.915542e-05 1 9262059 9262270 212 + 1.732 1.556 -0.599
ENSG00000049239 E012 17.087598 0.0011652168 2.690226e-03 9.344785e-03 1 9262271 9262328 58 + 1.343 1.163 -0.636
ENSG00000049239 E013 231.122368 0.0002043311 4.998393e-27 6.463690e-25 1 9263509 9265529 2021 + 2.446 2.277 -0.563
ENSG00000049239 E014 355.164189 0.0008912804 5.403523e-03 1.702447e-02 1 9265530 9268037 2508 + 2.527 2.541 0.046
ENSG00000049239 E015 681.844472 0.0028994083 1.109311e-30 1.962238e-28 1 9268038 9271144 3107 + 2.616 2.912 0.986
ENSG00000049239 E016 40.892062 0.0115746032 2.491229e-05 1.511420e-04 1 9271145 9271337 193 + 1.320 1.729 1.401