ENSG00000048991

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000409478 ENSG00000048991 HEK293_OSMI2_2hA HEK293_TMG_2hB R3HDM1 protein_coding protein_coding 24.40389 22.79291 22.96717 1.699569 0.9115591 0.01098328 7.8388853 6.96285833 6.4556103 0.90975999 1.3780466 -0.1089636 0.31985417 0.304500000 0.27750000 -0.02700000 0.875529136 0.001595339 FALSE TRUE
ENST00000429703 ENSG00000048991 HEK293_OSMI2_2hA HEK293_TMG_2hB R3HDM1 protein_coding protein_coding 24.40389 22.79291 22.96717 1.699569 0.9115591 0.01098328 2.7268125 7.46106495 0.2799064 1.01810805 0.2295892 -4.6876549 0.12348750 0.337200000 0.01296667 -0.32423333 0.001595339 0.001595339 FALSE TRUE
ENST00000445855 ENSG00000048991 HEK293_OSMI2_2hA HEK293_TMG_2hB R3HDM1 protein_coding protein_coding 24.40389 22.79291 22.96717 1.699569 0.9115591 0.01098328 0.7877432 0.39230493 1.6224114 0.39230493 0.0759100 2.0206434 0.03184583 0.016700000 0.07106667 0.05436667 0.124037265 0.001595339 FALSE FALSE
MSTRG.19284.3 ENSG00000048991 HEK293_OSMI2_2hA HEK293_TMG_2hB R3HDM1 protein_coding   24.40389 22.79291 22.96717 1.699569 0.9115591 0.01098328 2.3751835 1.83922687 3.5437905 0.98935908 1.7786954 0.9424364 0.10105417 0.076100000 0.15976667 0.08366667 0.907225968 0.001595339 FALSE TRUE
MSTRG.19284.7 ENSG00000048991 HEK293_OSMI2_2hA HEK293_TMG_2hB R3HDM1 protein_coding   24.40389 22.79291 22.96717 1.699569 0.9115591 0.01098328 1.3924392 0.03899487 2.0633702 0.03899487 1.0338355 5.4032034 0.05404167 0.001666667 0.08993333 0.08826667 0.298826345 0.001595339 FALSE TRUE
MSTRG.19284.8 ENSG00000048991 HEK293_OSMI2_2hA HEK293_TMG_2hB R3HDM1 protein_coding   24.40389 22.79291 22.96717 1.699569 0.9115591 0.01098328 0.8844783 0.02342980 1.3567372 0.02342980 1.3567372 5.3534575 0.03531250 0.001200000 0.05920000 0.05800000 0.999228787 0.001595339 FALSE TRUE
MSTRG.19284.9 ENSG00000048991 HEK293_OSMI2_2hA HEK293_TMG_2hB R3HDM1 protein_coding   24.40389 22.79291 22.96717 1.699569 0.9115591 0.01098328 3.0591202 3.13411883 2.6176958 1.06962905 1.3302397 -0.2588575 0.12310417 0.134200000 0.11353333 -0.02066667 0.868801510 0.001595339 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000048991 E001 0.1451727 0.0427632407 5.055530e-01   2 135531291 135531454 164 + 0.112 0.000 -19.354
ENSG00000048991 E002 0.2903454 0.2892800031 2.831496e-01   2 135531455 135531472 18 + 0.201 0.000 -20.355
ENSG00000048991 E003 0.4418608 0.0243818791 9.037677e-02 1.757108e-01 2 135531473 135531478 6 + 0.276 0.000 -20.944
ENSG00000048991 E004 0.5922303 0.0180113596 3.294742e-01 4.729882e-01 2 135531479 135531479 1 + 0.276 0.133 -1.309
ENSG00000048991 E005 0.7437457 0.0146011696 1.821826e-01 3.039393e-01 2 135531480 135531483 4 + 0.339 0.133 -1.724
ENSG00000048991 E006 4.8779293 0.0033722439 2.499640e-02 6.186628e-02 2 135531484 135531500 17 + 0.875 0.626 -1.012
ENSG00000048991 E007 12.3145205 0.0013895781 1.419735e-02 3.867260e-02 2 135531501 135531512 12 + 1.198 1.028 -0.611
ENSG00000048991 E008 13.3131226 0.0013185961 1.022515e-02 2.932016e-02 2 135531513 135531516 4 + 1.229 1.057 -0.619
ENSG00000048991 E009 18.3901566 0.0010473371 1.580903e-02 4.230905e-02 2 135531517 135531526 10 + 1.342 1.215 -0.445
ENSG00000048991 E010 45.8170380 0.0004766046 1.864059e-05 1.164711e-04 2 135531527 135531633 107 + 1.739 1.583 -0.529
ENSG00000048991 E011 1.8402886 0.0078153613 3.394779e-01 4.834007e-01 2 135565737 135565925 189 + 0.339 0.545 1.085
ENSG00000048991 E012 0.4418608 0.0243818791 9.037677e-02 1.757108e-01 2 135568758 135568809 52 + 0.276 0.000 -20.944
ENSG00000048991 E013 0.3332198 0.0290125241 9.819360e-01   2 135586250 135586322 73 + 0.112 0.133 0.278
ENSG00000048991 E014 0.4804688 0.0213595648 5.865985e-01 7.101324e-01 2 135586323 135586351 29 + 0.202 0.133 -0.722
ENSG00000048991 E015 0.7019140 0.0164734017 9.729325e-01 9.870992e-01 2 135586352 135586436 85 + 0.202 0.235 0.279
ENSG00000048991 E016 0.3686942 0.0337041968 9.786456e-01 9.907651e-01 2 135586437 135586506 70 + 0.112 0.133 0.281
ENSG00000048991 E017 109.5991785 0.0008563809 1.099894e-09 1.618169e-08 2 135602500 135602708 209 + 2.112 1.961 -0.506
ENSG00000048991 E018 72.5667179 0.0075073210 8.416482e-04 3.407585e-03 2 135604806 135604855 50 + 1.920 1.798 -0.411
ENSG00000048991 E019 120.3459426 0.0109969549 8.787105e-06 5.915535e-05 2 135604856 135605016 161 + 2.166 1.979 -0.625
ENSG00000048991 E020 7.3692081 0.0536893431 1.806387e-02 4.726786e-02 2 135605017 135606676 1660 + 1.055 0.748 -1.171
ENSG00000048991 E021 3.0132994 0.0058639755 1.290004e-02 3.567991e-02 2 135616152 135616193 42 + 0.735 0.386 -1.628
ENSG00000048991 E022 25.9342836 0.0069658369 6.818706e-03 2.075087e-02 2 135616668 135616757 90 + 1.493 1.353 -0.485
ENSG00000048991 E023 56.7307949 0.0234091468 1.649374e-03 6.119043e-03 2 135621494 135621511 18 + 1.844 1.657 -0.633
ENSG00000048991 E024 88.7141109 0.0003929192 4.478174e-07 3.999135e-06 2 135621512 135621608 97 + 2.006 1.882 -0.417
ENSG00000048991 E025 69.5875767 0.0012506984 6.130112e-02 1.288217e-01 2 135622654 135622732 79 + 1.852 1.836 -0.053
ENSG00000048991 E026 68.6824792 0.0011101261 6.347899e-05 3.484833e-04 2 135631718 135631777 60 + 1.891 1.780 -0.376
ENSG00000048991 E027 119.3839137 0.0050026851 3.395591e-05 1.992825e-04 2 135631861 135632001 141 + 2.135 2.012 -0.409
ENSG00000048991 E028 3.4989665 0.0045620648 7.616926e-03 2.281645e-02 2 135633712 135633828 117 + 0.801 0.446 -1.571
ENSG00000048991 E029 113.0077337 0.0021309813 5.368200e-04 2.297554e-03 2 135635890 135635998 109 + 2.085 2.018 -0.223
ENSG00000048991 E030 115.9479992 0.0026092775 1.982880e-03 7.173288e-03 2 135636088 135636183 96 + 2.091 2.036 -0.184
ENSG00000048991 E031 75.9079291 0.0031844157 3.115526e-03 1.060606e-02 2 135638618 135638640 23 + 1.921 1.848 -0.245
ENSG00000048991 E032 71.8744743 0.0028824936 2.544046e-03 8.895076e-03 2 135638641 135638655 15 + 1.899 1.823 -0.256
ENSG00000048991 E033 0.1817044 0.0388675711 4.719825e-01   2 135638733 135638738 6 + 0.000 0.133 19.640
ENSG00000048991 E034 94.1460574 0.0053276653 6.660967e-04 2.774541e-03 2 135638739 135638789 51 + 2.024 1.925 -0.331
ENSG00000048991 E035 57.6440322 0.0090491280 2.781073e-04 1.290579e-03 2 135638896 135638898 3 + 1.840 1.674 -0.562
ENSG00000048991 E036 156.2956745 0.0066456480 5.582277e-04 2.378324e-03 2 135638899 135639074 176 + 2.237 2.149 -0.294
ENSG00000048991 E037 82.4694013 0.0070845298 2.094831e-02 5.348079e-02 2 135639075 135639122 48 + 1.944 1.890 -0.180
ENSG00000048991 E038 31.6523801 0.0268451534 2.499938e-01 3.858955e-01 2 135641536 135641604 69 + 1.526 1.491 -0.120
ENSG00000048991 E039 38.0758185 0.0005702068 7.204148e-02 1.467269e-01 2 135641605 135641790 186 + 1.605 1.571 -0.119
ENSG00000048991 E040 0.9641468 0.0123849502 1.000590e-01 1.905744e-01 2 135645233 135645378 146 + 0.394 0.133 -2.043
ENSG00000048991 E041 120.6486597 0.0002832525 6.233861e-06 4.342659e-05 2 135645379 135645527 149 + 2.118 2.040 -0.263
ENSG00000048991 E042 2.0607158 0.3367015433 8.554026e-01 9.105917e-01 2 135649087 135649331 245 + 0.395 0.553 0.793
ENSG00000048991 E043 0.9232601 0.0791377580 4.644553e-01 6.041669e-01 2 135649902 135650003 102 + 0.339 0.234 -0.728
ENSG00000048991 E044 28.3317871 0.0038951824 2.808389e-01 4.207121e-01 2 135651730 135651732 3 + 1.472 1.462 -0.037
ENSG00000048991 E045 140.9218494 0.0016424394 2.691115e-02 6.580336e-02 2 135651733 135652032 300 + 2.151 2.143 -0.028
ENSG00000048991 E046 109.5344769 0.0010100988 8.872829e-03 2.596353e-02 2 135661270 135661393 124 + 2.054 2.027 -0.091
ENSG00000048991 E047 108.9652022 0.0004371934 2.378670e-01 3.716643e-01 2 135675332 135675397 66 + 2.025 2.052 0.092
ENSG00000048991 E048 145.5101588 0.0002317337 2.252741e-01 3.567447e-01 2 135675398 135675486 89 + 2.147 2.179 0.109
ENSG00000048991 E049 189.0559686 0.0002252100 7.235723e-01 8.173647e-01 2 135680173 135680324 152 + 2.246 2.306 0.198
ENSG00000048991 E050 167.3710636 0.0012404695 8.913457e-01 9.345854e-01 2 135709433 135709536 104 + 2.190 2.254 0.213
ENSG00000048991 E051 198.1359209 0.0012585350 5.874259e-02 1.244709e-01 2 135710059 135710231 173 + 2.229 2.354 0.416
ENSG00000048991 E052 191.2809325 0.0002541866 4.788738e-04 2.079049e-03 2 135715550 135715694 145 + 2.194 2.353 0.532
ENSG00000048991 E053 1.7941930 0.0709629912 8.291895e-01 8.927250e-01 2 135721509 135721923 415 + 0.443 0.447 0.021
ENSG00000048991 E054 153.2147557 0.0002558592 7.039521e-02 1.440081e-01 2 135721924 135722006 83 + 2.120 2.241 0.403
ENSG00000048991 E055 140.3610317 0.0027677639 9.006541e-03 2.629553e-02 2 135722469 135722553 85 + 2.056 2.227 0.569
ENSG00000048991 E056 998.8748828 0.0109763985 1.126893e-14 3.677630e-13 2 135723937 135725270 1334 + 2.755 3.156 1.332