ENSG00000048028

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000003302 ENSG00000048028 HEK293_OSMI2_2hA HEK293_TMG_2hB USP28 protein_coding protein_coding 13.78785 6.916599 19.57299 0.0955542 0.7107283 1.499382 1.6559084 2.2845250 1.680007 0.2733917 0.3918368 -0.4411665 0.15760417 0.33000000 0.08593333 -0.24406667 2.932538e-05 1.736459e-21 FALSE TRUE
ENST00000544750 ENSG00000048028 HEK293_OSMI2_2hA HEK293_TMG_2hB USP28 protein_coding protein_coding 13.78785 6.916599 19.57299 0.0955542 0.7107283 1.499382 0.5758338 0.1115384 1.557713 0.1115384 0.3174430 3.6891767 0.04026667 0.01583333 0.07860000 0.06276667 1.252079e-01 1.736459e-21   FALSE
ENST00000544967 ENSG00000048028 HEK293_OSMI2_2hA HEK293_TMG_2hB USP28 protein_coding protein_coding 13.78785 6.916599 19.57299 0.0955542 0.7107283 1.499382 1.4733030 3.1115776 0.412027 0.2765648 0.1161057 -2.8868681 0.18080417 0.45040000 0.02070000 -0.42970000 1.736459e-21 1.736459e-21 FALSE TRUE
MSTRG.6337.10 ENSG00000048028 HEK293_OSMI2_2hA HEK293_TMG_2hB USP28 protein_coding   13.78785 6.916599 19.57299 0.0955542 0.7107283 1.499382 0.9911689 0.2909205 1.977525 0.1217422 0.1523819 2.7235190 0.06124167 0.04196667 0.10180000 0.05983333 1.762389e-01 1.736459e-21 FALSE TRUE
MSTRG.6337.2 ENSG00000048028 HEK293_OSMI2_2hA HEK293_TMG_2hB USP28 protein_coding   13.78785 6.916599 19.57299 0.0955542 0.7107283 1.499382 5.4161584 0.2025663 8.399792 0.2025663 0.2875708 5.3060853 0.31949583 0.02876667 0.43096667 0.40220000 9.798478e-03 1.736459e-21 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000048028 E001 26.7657638 0.0138890720 8.042783e-12 1.699588e-10 11 113797874 113798091 218 - 1.118 1.734 2.133
ENSG00000048028 E002 204.4211655 0.0075599433 5.211794e-17 2.316656e-15 11 113798092 113798779 688 - 2.104 2.523 1.401
ENSG00000048028 E003 35.3886923 0.0191689837 5.083381e-02 1.106627e-01 11 113798780 113798804 25 - 1.459 1.660 0.688
ENSG00000048028 E004 89.0361315 0.0024023461 7.788850e-05 4.180571e-04 11 113798805 113798978 174 - 1.850 2.050 0.673
ENSG00000048028 E005 63.5407864 0.0004604490 7.731353e-03 2.310406e-02 11 113798979 113799061 83 - 1.727 1.875 0.502
ENSG00000048028 E006 60.8778058 0.0003726719 1.782547e-01 2.989617e-01 11 113799062 113799119 58 - 1.730 1.820 0.303
ENSG00000048028 E007 58.6267892 0.0003917178 1.834785e-01 3.055642e-01 11 113799120 113799171 52 - 1.715 1.805 0.305
ENSG00000048028 E008 117.3006788 0.0002995492 7.659726e-04 3.135912e-03 11 113799172 113799415 244 - 1.992 2.133 0.472
ENSG00000048028 E009 85.0951489 0.0010291095 1.995552e-04 9.619383e-04 11 113801483 113801678 196 - 1.839 2.018 0.605
ENSG00000048028 E010 0.9058703 0.7928906513 1.000000e+00 1.000000e+00 11 113801679 113801701 23 - 0.303 0.180 -0.972
ENSG00000048028 E011 72.4266030 0.0134260231 3.213740e-02 7.612511e-02 11 113803158 113803281 124 - 1.772 1.949 0.594
ENSG00000048028 E012 65.0552636 0.0223749234 5.158091e-01 6.504408e-01 11 113803798 113803877 80 - 1.760 1.848 0.300
ENSG00000048028 E013 62.7258502 0.0070149071 5.431105e-02 1.167898e-01 11 113804673 113804751 79 - 1.724 1.865 0.477
ENSG00000048028 E014 54.6802185 0.0026572525 3.307936e-01 4.743648e-01 11 113804868 113804927 60 - 1.688 1.767 0.269
ENSG00000048028 E015 75.9087699 0.0019279447 5.461327e-01 6.763671e-01 11 113804928 113805046 119 - 1.835 1.888 0.179
ENSG00000048028 E016 3.2514955 0.0048172463 9.865504e-01 9.956527e-01 11 113806489 113806584 96 - 0.600 0.623 0.100
ENSG00000048028 E017 2.9829320 0.0053065883 8.550155e-02 1.682141e-01 11 113808023 113808136 114 - 0.475 0.763 1.269
ENSG00000048028 E018 84.4766021 0.0004726278 7.523319e-01 8.385619e-01 11 113808298 113808437 140 - 1.884 1.922 0.130
ENSG00000048028 E019 107.8361953 0.0002774467 4.740522e-01 6.129177e-01 11 113809063 113809254 192 - 2.003 2.003 -0.002
ENSG00000048028 E020 36.1167239 0.0005986318 3.953549e-01 5.391700e-01 11 113812276 113812278 3 - 1.546 1.518 -0.097
ENSG00000048028 E021 78.5785584 0.0003661290 2.226240e-01 3.535302e-01 11 113812279 113812414 136 - 1.877 1.850 -0.091
ENSG00000048028 E022 58.2199290 0.0005112535 4.605853e-01 6.005844e-01 11 113812415 113812504 90 - 1.746 1.734 -0.037
ENSG00000048028 E023 1.3704194 0.0146683639 2.168899e-01 3.466501e-01 11 113813755 113813884 130 - 0.413 0.186 -1.577
ENSG00000048028 E024 48.8912971 0.0004514623 1.918460e-01 3.160789e-01 11 113813885 113813955 71 - 1.680 1.634 -0.157
ENSG00000048028 E025 66.0715768 0.0003619760 7.754724e-01 8.553488e-01 11 113815174 113815263 90 - 1.793 1.805 0.043
ENSG00000048028 E026 69.0351013 0.0003945074 9.118630e-01 9.480855e-01 11 113815264 113815376 113 - 1.809 1.830 0.070
ENSG00000048028 E027 29.7136269 0.0007042325 2.974520e-01 4.387951e-01 11 113815377 113815382 6 - 1.472 1.424 -0.163
ENSG00000048028 E028 44.2006293 0.0005304233 1.267724e-02 3.515111e-02 11 113817658 113817694 37 - 1.660 1.538 -0.415
ENSG00000048028 E029 36.0654651 0.0027384322 2.162387e-02 5.489404e-02 11 113817695 113817705 11 - 1.575 1.441 -0.459
ENSG00000048028 E030 61.6611344 0.0003743880 1.258915e-01 2.284169e-01 11 113817706 113817830 125 - 1.780 1.730 -0.167
ENSG00000048028 E031 25.5815941 0.0007623036 2.574443e-01 3.945640e-01 11 113817831 113817837 7 - 1.409 1.349 -0.209
ENSG00000048028 E032 2.4389659 0.0062242670 3.782227e-01 5.225021e-01 11 113820292 113820358 67 - 0.554 0.415 -0.693
ENSG00000048028 E033 56.5161053 0.0132281727 5.096779e-01 6.449772e-01 11 113823605 113823700 96 - 1.733 1.704 -0.097
ENSG00000048028 E034 67.5836424 0.0004486530 2.214700e-03 7.888994e-03 11 113827233 113827360 128 - 1.836 1.713 -0.417
ENSG00000048028 E035 1.4875932 0.0106879799 1.969848e-02 5.084845e-02 11 113828844 113828920 77 - 0.475 0.001 -10.658
ENSG00000048028 E036 44.1748803 0.0007175916 1.806895e-02 4.727891e-02 11 113829197 113829220 24 - 1.654 1.539 -0.395
ENSG00000048028 E037 70.9717056 0.0003314856 2.636249e-03 9.178157e-03 11 113829221 113829345 125 - 1.858 1.743 -0.389
ENSG00000048028 E038 1.0320003 0.0325410987 7.867886e-01 8.634597e-01 11 113829346 113829467 122 - 0.254 0.316 0.430
ENSG00000048028 E039 35.0221762 0.0005691272 8.789350e-04 3.540314e-03 11 113830867 113830882 16 - 1.579 1.379 -0.691
ENSG00000048028 E040 46.3360474 0.0005221870 1.060643e-02 3.022542e-02 11 113830883 113830943 61 - 1.680 1.558 -0.416
ENSG00000048028 E041 7.5866021 0.0130712119 4.372659e-01 5.792472e-01 11 113830944 113830946 3 - 0.922 0.837 -0.324
ENSG00000048028 E042 3.1371947 0.1813018748 3.703947e-01 5.148263e-01 11 113830947 113830976 30 - 0.643 0.418 -1.069
ENSG00000048028 E043 42.7227051 0.0004921374 4.757048e-01 6.143961e-01 11 113831920 113831989 70 - 1.616 1.601 -0.053
ENSG00000048028 E044 23.9685608 0.0021451066 5.233652e-01 6.570748e-01 11 113831990 113831993 4 - 1.374 1.349 -0.088
ENSG00000048028 E045 75.6108010 0.0011016395 4.788330e-04 2.078963e-03 11 113833420 113833557 138 - 1.893 1.751 -0.478
ENSG00000048028 E046 59.3130564 0.0021498194 1.390481e-04 6.994383e-04 11 113834249 113834335 87 - 1.798 1.607 -0.649
ENSG00000048028 E047 1.7026957 0.0081777418 1.190237e-01 2.185521e-01 11 113835207 113835327 121 - 0.475 0.186 -1.899
ENSG00000048028 E048 84.6707905 0.0123875264 1.529042e-05 9.739510e-05 11 113840598 113840757 160 - 1.971 1.688 -0.953
ENSG00000048028 E049 70.5411536 0.0007379757 1.520126e-10 2.600535e-09 11 113841663 113841765 103 - 1.899 1.595 -1.030
ENSG00000048028 E050 32.4779725 0.0006066177 2.240063e-06 1.723903e-05 11 113841766 113841768 3 - 1.570 1.243 -1.131
ENSG00000048028 E051 37.6333092 0.0007209815 2.564993e-06 1.949726e-05 11 113852501 113852526 26 - 1.627 1.327 -1.030
ENSG00000048028 E052 48.9350829 0.0018496348 1.350240e-03 5.144005e-03 11 113852527 113852621 95 - 1.713 1.545 -0.572
ENSG00000048028 E053 24.5326694 0.0034236317 1.104913e-01 2.062471e-01 11 113852622 113852633 12 - 1.409 1.304 -0.363
ENSG00000048028 E054 40.8628367 0.0006926926 8.933261e-03 2.611213e-02 11 113854258 113854335 78 - 1.627 1.489 -0.470
ENSG00000048028 E055 11.9831190 0.0124501754 4.258992e-01 5.687673e-01 11 113874517 113874626 110 - 1.098 1.026 -0.261
ENSG00000048028 E056 11.4296130 0.0300010680 2.412183e-01 3.756150e-01 11 113874627 113874762 136 - 1.098 0.957 -0.520
ENSG00000048028 E057 14.9315648 0.0063734793 2.410821e-03 8.494640e-03 11 113874763 113874800 38 - 1.243 0.928 -1.143
ENSG00000048028 E058 15.6733360 0.0166399646 1.366551e-03 5.198053e-03 11 113874801 113874879 79 - 1.272 0.901 -1.346
ENSG00000048028 E059 23.0593407 0.0011351792 3.756239e-03 1.245747e-02 11 113875445 113875570 126 - 1.406 1.187 -0.765