ENSG00000046653

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000316715 ENSG00000046653 HEK293_OSMI2_2hA HEK293_TMG_2hB GPM6B protein_coding protein_coding 7.282562 2.98919 12.21112 0.4378345 0.09077589 2.026731 0.4822737 0.75246293 0.7466391 0.08190814 0.1455658 -0.01106189 0.10801250 0.26323333 0.06110000 -0.20213333 0.0002382869 0.0002382869 FALSE TRUE
ENST00000454189 ENSG00000046653 HEK293_OSMI2_2hA HEK293_TMG_2hB GPM6B protein_coding protein_coding 7.282562 2.98919 12.21112 0.4378345 0.09077589 2.026731 4.1272313 1.82189514 7.3981015 0.08004264 0.5575216 2.01576695 0.58579583 0.62823333 0.60643333 -0.02180000 0.9640622342 0.0002382869 FALSE TRUE
ENST00000472735 ENSG00000046653 HEK293_OSMI2_2hA HEK293_TMG_2hB GPM6B protein_coding processed_transcript 7.282562 2.98919 12.21112 0.4378345 0.09077589 2.026731 0.1836437 0.03935328 1.1535607 0.03935328 0.5768802 4.55925673 0.01602917 0.01030000 0.09376667 0.08346667 0.6213142478 0.0002382869 FALSE TRUE
MSTRG.33919.2 ENSG00000046653 HEK293_OSMI2_2hA HEK293_TMG_2hB GPM6B protein_coding   7.282562 2.98919 12.21112 0.4378345 0.09077589 2.026731 0.8678690 0.37547844 1.5696773 0.37547844 0.3355536 2.03490776 0.13124583 0.09823333 0.12826667 0.03003333 0.4719071840 0.0002382869 TRUE TRUE
MSTRG.33919.4 ENSG00000046653 HEK293_OSMI2_2hA HEK293_TMG_2hB GPM6B protein_coding   7.282562 2.98919 12.21112 0.4378345 0.09077589 2.026731 0.7866584 0.00000000 0.7687779 0.00000000 0.4165767 6.28314008 0.06355833 0.00000000 0.06290000 0.06290000 0.4076405910 0.0002382869 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000046653 E001 8.8486181 0.0020395135 1.750224e-07 1.696555e-06 X 13770939 13771100 162 - 0.703 1.276 2.144
ENSG00000046653 E002 38.1162468 0.0170955534 2.515758e-07 2.365395e-06 X 13771101 13771455 355 - 1.376 1.819 1.510
ENSG00000046653 E003 105.6938669 0.0003575417 4.773389e-09 6.253833e-08 X 13771456 13772100 645 - 1.915 2.125 0.705
ENSG00000046653 E004 103.9666035 0.0003581493 6.975514e-01 7.976497e-01 X 13772101 13772698 598 - 1.978 1.967 -0.039
ENSG00000046653 E005 50.2389065 0.0004772658 4.863096e-01 6.240846e-01 X 13772699 13772853 155 - 1.675 1.640 -0.119
ENSG00000046653 E006 58.5411091 0.0040675300 2.470689e-02 6.127623e-02 X 13772854 13773030 177 - 1.764 1.628 -0.464
ENSG00000046653 E007 115.1186490 0.0037315059 4.277336e-01 5.705695e-01 X 13773031 13773864 834 - 2.029 1.992 -0.123
ENSG00000046653 E008 37.7151589 0.0103696180 9.861463e-01 9.953407e-01 X 13773865 13773953 89 - 1.541 1.544 0.007
ENSG00000046653 E009 71.3928027 0.0024030575 4.332302e-01 5.756339e-01 X 13773954 13774308 355 - 1.800 1.839 0.132
ENSG00000046653 E010 29.2307918 0.0007079795 2.759994e-01 4.154002e-01 X 13774309 13774336 28 - 1.412 1.491 0.272
ENSG00000046653 E011 46.5523030 0.0005338345 9.694796e-01 9.849128e-01 X 13774337 13774466 130 - 1.629 1.634 0.018
ENSG00000046653 E012 64.9056305 0.0018040170 8.266581e-01 8.909211e-01 X 13774467 13774624 158 - 1.778 1.771 -0.023
ENSG00000046653 E013 0.3030308 0.3751485835 1.000000e+00   X 13776167 13776237 71 - 0.137 0.000 -9.525
ENSG00000046653 E014 62.8345034 0.0073997631 7.981213e-01 8.711581e-01 X 13776238 13776303 66 - 1.765 1.763 -0.006
ENSG00000046653 E015 56.2750007 0.0033861848 6.236901e-01 7.402112e-01 X 13777352 13777425 74 - 1.723 1.701 -0.073
ENSG00000046653 E016 44.8021623 0.0077100473 5.305959e-01 6.631964e-01 X 13779818 13779852 35 - 1.629 1.594 -0.120
ENSG00000046653 E017 48.5974814 0.0005920894 1.178915e-02 3.302799e-02 X 13779853 13779907 55 - 1.688 1.544 -0.492
ENSG00000046653 E018 46.1456671 0.0051568142 3.961531e-01 5.399438e-01 X 13779908 13779989 82 - 1.643 1.590 -0.180
ENSG00000046653 E019 0.2966881 0.0270295446 7.955634e-01   X 13780915 13781005 91 - 0.136 0.000 -9.518
ENSG00000046653 E020 46.5882606 0.0007161156 2.514760e-01 3.877223e-01 X 13783365 13783448 84 - 1.649 1.585 -0.218
ENSG00000046653 E021 43.1982601 0.0006375311 9.669372e-04 3.845078e-03 X 13783449 13783521 73 - 1.649 1.439 -0.717
ENSG00000046653 E022 0.0000000       X 13783779 13783921 143 -      
ENSG00000046653 E023 65.2526979 0.0084980767 2.681631e-02 6.561027e-02 X 13785622 13785808 187 - 1.813 1.661 -0.516
ENSG00000046653 E024 1.6274674 0.0145113933 2.008104e-01 3.272004e-01 X 13807650 13807769 120 - 0.453 0.197 -1.674
ENSG00000046653 E025 0.0000000       X 13812871 13812908 38 -      
ENSG00000046653 E026 0.0000000       X 13816844 13817195 352 -      
ENSG00000046653 E027 0.0000000       X 13817196 13817257 62 -      
ENSG00000046653 E028 0.0000000       X 13817258 13817346 89 -      
ENSG00000046653 E029 0.0000000       X 13888981 13889057 77 -      
ENSG00000046653 E030 0.0000000       X 13889452 13889612 161 -      
ENSG00000046653 E031 0.2903454 0.3596980713 1.000000e+00   X 13938327 13938506 180 - 0.136 0.000 -9.513
ENSG00000046653 E032 34.4123702 0.0006256686 1.757705e-02 4.621614e-02 X 13938507 13938797 291 - 1.544 1.381 -0.561