ENSG00000046647

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000332885 ENSG00000046647 HEK293_OSMI2_2hA HEK293_TMG_2hB GEMIN8 protein_coding protein_coding 12.34123 15.62204 8.140037 1.225813 0.174126 -0.9396278 0.4494941 0.1530239 0.6523025 0.04480834 0.07113529 2.0224072 0.04182500 0.01030000 0.08056667 0.070266667 3.948775e-07 3.948775e-07 FALSE FALSE
ENST00000398355 ENSG00000046647 HEK293_OSMI2_2hA HEK293_TMG_2hB GEMIN8 protein_coding protein_coding 12.34123 15.62204 8.140037 1.225813 0.174126 -0.9396278 6.6321422 9.0988529 2.9150446 0.75988989 0.17769355 -1.6388108 0.51857083 0.58256667 0.35850000 -0.224066667 1.147894e-05 3.948775e-07 FALSE TRUE
ENST00000477386 ENSG00000046647 HEK293_OSMI2_2hA HEK293_TMG_2hB GEMIN8 protein_coding protein_coding 12.34123 15.62204 8.140037 1.225813 0.174126 -0.9396278 0.2848878 0.1526433 0.5129785 0.08546506 0.07026035 1.6850406 0.02840000 0.01060000 0.06280000 0.052200000 1.670165e-02 3.948775e-07 FALSE TRUE
ENST00000680255 ENSG00000046647 HEK293_OSMI2_2hA HEK293_TMG_2hB GEMIN8 protein_coding protein_coding 12.34123 15.62204 8.140037 1.225813 0.174126 -0.9396278 0.6942335 1.0250793 0.2559707 0.31364643 0.14964393 -1.9604020 0.05291667 0.06326667 0.03223333 -0.031033333 5.757895e-01 3.948775e-07 FALSE TRUE
MSTRG.33915.3 ENSG00000046647 HEK293_OSMI2_2hA HEK293_TMG_2hB GEMIN8 protein_coding   12.34123 15.62204 8.140037 1.225813 0.174126 -0.9396278 2.0363271 3.2680669 1.7502546 0.50269678 0.69805960 -0.8970612 0.16522500 0.21046667 0.21413333 0.003666667 9.530410e-01 3.948775e-07 FALSE TRUE
MSTRG.33915.5 ENSG00000046647 HEK293_OSMI2_2hA HEK293_TMG_2hB GEMIN8 protein_coding   12.34123 15.62204 8.140037 1.225813 0.174126 -0.9396278 1.3248698 0.9192164 1.2023130 0.12819865 0.51910818 0.3836756 0.11281667 0.06036667 0.14790000 0.087533333 3.879221e-01 3.948775e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000046647 E001 0.5149242 0.2464282752 8.785285e-01 9.259793e-01 X 14006726 14006747 22 - 0.176 0.154 -0.227
ENSG00000046647 E002 31.3745988 0.0007507537 9.527063e-02 1.832818e-01 X 14006748 14007595 848 - 1.543 1.430 -0.389
ENSG00000046647 E003 2.4585537 0.0104760545 8.894171e-01 9.333127e-01 X 14007596 14007611 16 - 0.476 0.495 0.094
ENSG00000046647 E004 12.1580081 0.0202337936 4.965117e-01 6.331457e-01 X 14007612 14007813 202 - 1.129 1.042 -0.316
ENSG00000046647 E005 6.8024525 0.0073190119 3.795706e-02 8.726760e-02 X 14007814 14007832 19 - 1.020 0.754 -1.018
ENSG00000046647 E006 6.7043944 0.0026305652 1.046509e-01 1.975882e-01 X 14007833 14007870 38 - 0.977 0.770 -0.794
ENSG00000046647 E007 3.4298999 0.1650641634 5.813375e-01 7.058789e-01 X 14007871 14008278 408 - 0.698 0.585 -0.487
ENSG00000046647 E008 16.6192157 0.0302161578 6.397485e-01 7.530847e-01 X 14008279 14008672 394 - 1.241 1.191 -0.179
ENSG00000046647 E009 63.3812729 0.0005527419 1.827899e-03 6.689659e-03 X 14008673 14008870 198 - 1.647 1.796 0.504
ENSG00000046647 E010 50.4095474 0.0011840255 1.449130e-01 2.547283e-01 X 14008871 14008898 28 - 1.606 1.680 0.251
ENSG00000046647 E011 121.1353414 0.0003488684 2.378095e-06 1.819767e-05 X 14008899 14009110 212 - 1.913 2.072 0.537
ENSG00000046647 E012 64.3396225 0.0003752221 4.550752e-02 1.011244e-01 X 14009111 14009169 59 - 1.698 1.787 0.303
ENSG00000046647 E013 3.6534214 0.0336913327 6.217647e-01 7.386990e-01 X 14014208 14014536 329 - 0.698 0.618 -0.341
ENSG00000046647 E014 217.8644181 0.0056354459 5.104628e-01 6.456598e-01 X 14020078 14020534 457 - 2.310 2.277 -0.108
ENSG00000046647 E015 2.6560153 0.1997694482 1.665157e-01 2.836785e-01 X 14020535 14020587 53 - 0.739 0.400 -1.567
ENSG00000046647 E016 60.3987991 0.0004850454 6.515636e-01 7.624166e-01 X 14021464 14021511 48 - 1.755 1.726 -0.096
ENSG00000046647 E017 2.4817255 0.0064218494 8.477876e-03 2.498068e-02 X 14026052 14026139 88 - 0.777 0.357 -1.969
ENSG00000046647 E018 50.2007895 0.0005630744 5.847694e-02 1.240250e-01 X 14026140 14026221 82 - 1.731 1.627 -0.352
ENSG00000046647 E019 5.7667209 0.0029251115 1.221531e-07 1.220481e-06 X 14027009 14027169 161 - 1.160 0.495 -2.664
ENSG00000046647 E020 4.9435821 0.0031931967 3.274735e-08 3.660543e-07 X 14029394 14029776 383 - 1.129 0.396 -3.067
ENSG00000046647 E021 42.5860468 0.0005058505 4.080458e-01 5.516367e-01 X 14029777 14029893 117 - 1.627 1.574 -0.180