ENSG00000044574

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000324460 ENSG00000044574 HEK293_OSMI2_2hA HEK293_TMG_2hB HSPA5 protein_coding protein_coding 273.0013 207.1029 369.2032 6.313229 5.966098 0.8340369 98.23556 89.92335 124.0027 4.171391 3.45599 0.4635594 0.3709792 0.4340333 0.3357667 -0.09826667 1.478724e-05 1.478724e-05 FALSE  
ENST00000680032 ENSG00000044574 HEK293_OSMI2_2hA HEK293_TMG_2hB HSPA5 protein_coding protein_coding 273.0013 207.1029 369.2032 6.313229 5.966098 0.8340369 171.80479 115.94486 241.1763 3.807498 2.27920 1.0565844 0.6184292 0.5601000 0.6534000 0.09330000 2.087992e-04 1.478724e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000044574 E001 1.319990 0.0606416461 8.520129e-04 3.444837e-03 9 125234853 125234859 7 - 0.000 0.607 12.449
ENSG00000044574 E002 2164.287028 0.0006069724 7.957894e-30 1.318984e-27 9 125234860 125236488 1629 - 3.257 3.412 0.513
ENSG00000044574 E003 2039.445039 0.0003364808 2.121316e-32 4.243685e-30 9 125236489 125236561 73 - 3.243 3.378 0.448
ENSG00000044574 E004 1330.981948 0.0007244821 2.412708e-20 1.605481e-18 9 125236562 125236577 16 - 3.051 3.198 0.489
ENSG00000044574 E005 7001.340630 0.0003148510 3.439912e-22 2.792121e-20 9 125236578 125237061 484 - 3.808 3.884 0.252
ENSG00000044574 E006 2836.335406 0.0003298067 1.737098e-02 4.575814e-02 9 125237062 125237154 93 - 3.443 3.465 0.076
ENSG00000044574 E007 29.855184 0.0015493755 5.370423e-01 6.686980e-01 9 125237155 125238140 986 - 1.511 1.469 -0.144
ENSG00000044574 E008 3306.814768 0.0002621453 5.070998e-03 1.612456e-02 9 125238141 125238308 168 - 3.509 3.532 0.079
ENSG00000044574 E009 21.456834 0.0029673983 1.468177e-02 3.977245e-02 9 125238309 125238589 281 - 1.430 1.234 -0.682
ENSG00000044574 E010 2789.149083 0.0004729991 3.802947e-01 5.245790e-01 9 125238590 125238713 124 - 3.452 3.437 -0.049
ENSG00000044574 E011 2418.085383 0.0004943193 9.341104e-02 1.804507e-01 9 125238714 125238827 114 - 3.394 3.369 -0.084
ENSG00000044574 E012 7.072166 0.0032257637 1.540458e-01 2.671725e-01 9 125238828 125238940 113 - 0.980 0.805 -0.666
ENSG00000044574 E013 4808.542002 0.0001717371 7.841729e-24 7.638006e-22 9 125238941 125239331 391 - 3.716 3.637 -0.262
ENSG00000044574 E014 7.313536 0.0023204493 2.590792e-03 9.037671e-03 9 125239332 125239420 89 - 1.060 0.685 -1.446
ENSG00000044574 E015 2277.638103 0.0002620279 7.172522e-24 7.022288e-22 9 125239421 125239533 113 - 3.405 3.295 -0.365
ENSG00000044574 E016 11.897690 0.0079871912 5.387596e-01 6.701392e-01 9 125239534 125240171 638 - 1.145 1.083 -0.221
ENSG00000044574 E017 2104.450797 0.0009239465 1.972617e-11 3.912746e-10 9 125240172 125240309 138 - 3.372 3.258 -0.380
ENSG00000044574 E018 9.291299 0.0040505408 9.167681e-03 2.670259e-02 9 125240310 125240675 366 - 1.127 0.830 -1.105
ENSG00000044574 E019 2420.934756 0.0011314716 1.969976e-23 1.832954e-21 9 125240676 125240907 232 - 3.457 3.279 -0.591
ENSG00000044574 E020 4.968597 0.0102442366 7.595725e-01 8.439037e-01 9 125240908 125241004 97 - 0.795 0.749 -0.181
ENSG00000044574 E021 2205.455380 0.0025775520 1.117351e-04 5.757773e-04 9 125241005 125241382 378 - 3.387 3.286 -0.337