ENSG00000040275

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000265295 ENSG00000040275 HEK293_OSMI2_2hA HEK293_TMG_2hB SPDL1 protein_coding protein_coding 41.73447 14.79797 68.43348 0.8908495 2.703965 2.208539 27.429682 11.2044028 40.742478 0.6207590 1.3626245 1.861535 0.67352083 0.75880000 0.59710000 -0.161700000 3.371484e-02 2.457307e-16 FALSE TRUE
ENST00000507232 ENSG00000040275 HEK293_OSMI2_2hA HEK293_TMG_2hB SPDL1 protein_coding nonsense_mediated_decay 41.73447 14.79797 68.43348 0.8908495 2.703965 2.208539 3.027836 0.8191987 4.102452 0.1113457 0.1141573 2.310209 0.07406667 0.05523333 0.06016667 0.004933333 8.311326e-01 2.457307e-16 FALSE TRUE
ENST00000629457 ENSG00000040275 HEK293_OSMI2_2hA HEK293_TMG_2hB SPDL1 protein_coding protein_coding 41.73447 14.79797 68.43348 0.8908495 2.703965 2.208539 2.017580 0.0000000 6.768068 0.0000000 0.9944117 9.404730 0.03254167 0.00000000 0.09880000 0.098800000 2.457307e-16 2.457307e-16   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000040275 E001 0.7406253 0.0153956332 2.540546e-01 3.906836e-01 5 169583636 169583705 70 + 0.260 0.000 -9.530
ENSG00000040275 E002 1.6232010 0.0083733268 2.739682e-01 4.130638e-01 5 169583706 169583708 3 + 0.422 0.216 -1.349
ENSG00000040275 E003 4.0871459 0.0087645333 3.870361e-01 5.311893e-01 5 169583709 169583734 26 + 0.679 0.554 -0.550
ENSG00000040275 E004 4.6762558 0.0117521727 2.331740e-01 3.661958e-01 5 169583735 169583737 3 + 0.735 0.554 -0.782
ENSG00000040275 E005 7.1935574 0.0023389626 4.412915e-01 5.829290e-01 5 169583738 169583749 12 + 0.870 0.789 -0.312
ENSG00000040275 E006 8.0814317 0.0020303279 2.372848e-01 3.710301e-01 5 169583750 169583755 6 + 0.924 0.789 -0.519
ENSG00000040275 E007 8.2329471 0.0020857559 2.125386e-01 3.415462e-01 5 169583756 169583759 4 + 0.932 0.789 -0.550
ENSG00000040275 E008 15.0773808 0.0015843815 6.268008e-03 1.931418e-02 5 169583760 169583772 13 + 1.194 0.908 -1.043
ENSG00000040275 E009 34.8847117 0.0018502845 2.007819e-04 9.670886e-04 5 169583773 169583786 14 + 1.534 1.265 -0.929
ENSG00000040275 E010 34.1255388 0.0024660685 9.754982e-05 5.105398e-04 5 169583787 169583787 1 + 1.527 1.234 -1.018
ENSG00000040275 E011 147.3627369 0.0003658259 2.270302e-09 3.150468e-08 5 169583788 169583889 102 + 2.138 1.942 -0.657
ENSG00000040275 E012 2.5672754 0.0557001986 1.020740e-01 1.936628e-01 5 169584006 169584020 15 + 0.559 0.217 -2.018
ENSG00000040275 E013 2.3102395 0.0223301628 8.915912e-01 9.347652e-01 5 169584021 169584028 8 + 0.473 0.468 -0.025
ENSG00000040275 E014 4.4891376 0.0038576867 5.662117e-01 6.932221e-01 5 169584029 169584087 59 + 0.694 0.626 -0.289
ENSG00000040275 E015 8.5285896 0.0019670688 1.898165e-01 3.135314e-01 5 169584088 169584166 79 + 0.940 0.790 -0.581
ENSG00000040275 E016 12.2126446 0.0287524148 8.699422e-01 9.203343e-01 5 169585951 169586398 448 + 1.049 1.056 0.027
ENSG00000040275 E017 1.8802474 0.1552625693 7.943490e-01 8.686120e-01 5 169587172 169587198 27 + 0.422 0.363 -0.330
ENSG00000040275 E018 8.4152538 0.0374402490 6.343098e-01 7.488014e-01 5 169587199 169587258 60 + 0.880 0.975 0.360
ENSG00000040275 E019 6.7209771 0.0511835691 3.551272e-01 4.994823e-01 5 169587259 169587260 2 + 0.774 0.944 0.657
ENSG00000040275 E020 12.0847639 0.0220379001 7.259330e-01 8.190839e-01 5 169587261 169587372 112 + 1.030 1.102 0.263
ENSG00000040275 E021 0.8942170 0.0136494384 1.818812e-01 3.035818e-01 5 169588373 169588393 21 + 0.298 0.000 -11.307
ENSG00000040275 E022 200.6063999 0.0001880723 2.299138e-09 3.188382e-08 5 169588394 169588490 97 + 2.264 2.107 -0.525
ENSG00000040275 E023 148.0739810 0.0002367402 4.337846e-03 1.409084e-02 5 169588491 169588534 44 + 2.115 2.047 -0.230
ENSG00000040275 E024 136.3356584 0.0002834911 4.796719e-03 1.536564e-02 5 169588535 169588575 41 + 2.082 2.010 -0.242
ENSG00000040275 E025 10.3467762 0.0138481602 2.402373e-02 5.986482e-02 5 169588576 169588843 268 + 1.042 0.742 -1.150
ENSG00000040275 E026 7.7004424 0.0035089275 3.292124e-01 4.727199e-01 5 169590753 169590755 3 + 0.898 0.790 -0.419
ENSG00000040275 E027 20.9775961 0.0012570069 3.641470e-01 5.085258e-01 5 169590756 169590829 74 + 1.284 1.234 -0.177
ENSG00000040275 E028 179.7094484 0.0001873801 5.224869e-04 2.243762e-03 5 169591048 169591103 56 + 2.202 2.122 -0.265
ENSG00000040275 E029 242.8046944 0.0001627512 6.109263e-02 1.284738e-01 5 169591104 169591224 121 + 2.318 2.300 -0.059
ENSG00000040275 E030 125.7510549 0.0002845999 8.640506e-01 9.163397e-01 5 169593354 169593357 4 + 2.022 2.049 0.090
ENSG00000040275 E031 322.8322081 0.0002048898 6.623039e-03 2.024713e-02 5 169593358 169593548 191 + 2.445 2.413 -0.104
ENSG00000040275 E032 291.4498589 0.0001530724 1.384924e-02 3.788623e-02 5 169594145 169594294 150 + 2.400 2.372 -0.092
ENSG00000040275 E033 218.7247463 0.0003295814 5.045364e-02 1.099767e-01 5 169594394 169594492 99 + 2.275 2.251 -0.080
ENSG00000040275 E034 285.7375115 0.0002514166 4.884354e-02 1.071007e-01 5 169594571 169594681 111 + 2.389 2.373 -0.056
ENSG00000040275 E035 1.8540974 0.0100614842 2.217479e-01 3.524817e-01 5 169595274 169595291 18 + 0.448 0.216 -1.485
ENSG00000040275 E036 10.9310849 0.1869318784 1.939809e-01 3.187978e-01 5 169595610 169596178 569 + 1.042 0.857 -0.692
ENSG00000040275 E037 166.7699085 0.0023316767 6.638420e-01 7.718118e-01 5 169596561 169596584 24 + 2.146 2.163 0.057
ENSG00000040275 E038 252.6153323 0.0014493408 2.409392e-01 3.752761e-01 5 169596585 169596701 117 + 2.313 2.383 0.232
ENSG00000040275 E039 203.7111743 0.0042188547 4.011831e-01 5.448826e-01 5 169598476 169598579 104 + 2.221 2.289 0.227
ENSG00000040275 E040 293.4455860 0.0010148143 2.882111e-02 6.964823e-02 5 169598972 169599159 188 + 2.372 2.465 0.311
ENSG00000040275 E041 101.6711578 0.0003105296 1.952740e-02 5.047285e-02 5 169601280 169601281 2 + 1.905 2.031 0.424
ENSG00000040275 E042 416.2532079 0.0009170003 8.350029e-06 5.648389e-05 5 169601282 169601625 344 + 2.513 2.650 0.456
ENSG00000040275 E043 28.8848454 0.0007026389 4.732111e-02 1.044038e-01 5 169601626 169602021 396 + 1.356 1.530 0.602
ENSG00000040275 E044 2.4387373 0.1731128925 5.281153e-01 6.610190e-01 5 169604037 169604059 23 + 0.448 0.619 0.805
ENSG00000040275 E045 500.2611073 0.0089248991 2.304596e-07 2.183867e-06 5 169604060 169604778 719 + 2.550 2.818 0.893