ENSG00000037474

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264670 ENSG00000037474 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN2 protein_coding protein_coding 66.30766 54.83757 87.76253 2.580303 1.663584 0.6783418 50.237244 47.580448 62.42447 2.3024437 1.4917981 0.3916707 0.7617292 0.86760000 0.7113667 -0.15623333 1.271744e-13 1.271744e-13 FALSE TRUE
ENST00000506139 ENSG00000037474 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN2 protein_coding protein_coding 66.30766 54.83757 87.76253 2.580303 1.663584 0.6783418 8.501397 3.761618 14.73453 0.6164182 0.8503255 1.9669248 0.1179958 0.06906667 0.1679000 0.09883333 4.218781e-04 1.271744e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000037474 E001 3.752405 0.0042433617 1.740571e-07 1.688580e-06 5 6599239 6599239 1 - 0.107 0.951 4.826
ENSG00000037474 E002 3.752405 0.0042433617 1.740571e-07 1.688580e-06 5 6599240 6599240 1 - 0.107 0.951 4.826
ENSG00000037474 E003 342.361435 0.0035550825 9.691292e-14 2.762543e-12 5 6599241 6599399 159 - 2.386 2.662 0.921
ENSG00000037474 E004 469.034238 0.0035075190 5.737168e-22 4.553369e-20 5 6599400 6599568 169 - 2.483 2.819 1.117
ENSG00000037474 E005 1230.822107 0.0013442831 1.024514e-16 4.402156e-15 5 6599569 6600029 461 - 3.001 3.174 0.575
ENSG00000037474 E006 887.128623 0.0002102661 2.583492e-07 2.422705e-06 5 6600030 6600232 203 - 2.909 2.992 0.275
ENSG00000037474 E007 457.059953 0.0001375928 3.904412e-07 3.526816e-06 5 6602461 6602500 40 - 2.612 2.713 0.337
ENSG00000037474 E008 8.375083 0.0089941721 5.036438e-02 1.098162e-01 5 6603869 6604137 269 - 1.066 0.848 -0.817
ENSG00000037474 E009 666.358349 0.0006366006 1.218800e-05 7.936546e-05 5 6604138 6604276 139 - 2.780 2.873 0.310
ENSG00000037474 E010 531.707943 0.0009350728 4.185142e-02 9.445137e-02 5 6604605 6604685 81 - 2.700 2.755 0.183
ENSG00000037474 E011 33.806594 0.0043063507 5.641284e-04 2.399579e-03 5 6604686 6604800 115 - 1.633 1.408 -0.771
ENSG00000037474 E012 44.035021 0.0045729263 9.968939e-11 1.759279e-09 5 6604801 6605272 472 - 1.804 1.394 -1.399
ENSG00000037474 E013 350.418293 0.0013632515 8.282123e-01 8.920489e-01 5 6605273 6605286 14 - 2.538 2.556 0.059
ENSG00000037474 E014 613.147733 0.0019674902 3.971731e-01 5.409603e-01 5 6605287 6605408 122 - 2.793 2.782 -0.037
ENSG00000037474 E015 510.242220 0.0004850239 6.947140e-01 7.954807e-01 5 6606820 6606912 93 - 2.706 2.713 0.021
ENSG00000037474 E016 5.042696 0.0070818536 6.474786e-02 1.346331e-01 5 6606913 6606926 14 - 0.887 0.644 -0.981
ENSG00000037474 E017 755.250946 0.0004130528 2.480798e-01 3.836663e-01 5 6607200 6607384 185 - 2.881 2.876 -0.017
ENSG00000037474 E018 585.775416 0.0001070494 7.593638e-02 1.530091e-01 5 6609826 6609922 97 - 2.776 2.764 -0.040
ENSG00000037474 E019 610.073701 0.0001244361 1.241262e-07 1.238431e-06 5 6610955 6611085 131 - 2.817 2.752 -0.214
ENSG00000037474 E020 426.909313 0.0001468595 1.060779e-03 4.169235e-03 5 6611725 6611798 74 - 2.653 2.609 -0.145
ENSG00000037474 E021 542.102234 0.0001628590 8.849468e-03 2.590557e-02 5 6616727 6616857 131 - 2.750 2.722 -0.091
ENSG00000037474 E022 446.231291 0.0001267246 2.391185e-03 8.433451e-03 5 6617950 6618024 75 - 2.670 2.632 -0.125
ENSG00000037474 E023 277.599599 0.0001740283 3.669672e-02 8.490057e-02 5 6620106 6620115 10 - 2.461 2.430 -0.103
ENSG00000037474 E024 639.440031 0.0015773033 4.164182e-03 1.360346e-02 5 6620116 6620298 183 - 2.832 2.778 -0.179
ENSG00000037474 E025 29.629334 0.0023473700 1.219838e-12 2.953286e-11 5 6620299 6621489 1191 - 1.655 1.142 -1.781
ENSG00000037474 E026 9.476928 0.0179248620 1.651749e-02 4.388183e-02 5 6621603 6621744 142 - 1.134 0.848 -1.061
ENSG00000037474 E027 408.407010 0.0001657468 4.847510e-09 6.340857e-08 5 6622016 6622100 85 - 2.655 2.562 -0.308
ENSG00000037474 E028 377.856486 0.0004522087 8.163133e-08 8.435504e-07 5 6623214 6623285 72 - 2.622 2.524 -0.325
ENSG00000037474 E029 381.341048 0.0019435477 2.342448e-05 1.429394e-04 5 6625564 6625669 106 - 2.629 2.522 -0.358
ENSG00000037474 E030 221.396759 0.0017699476 9.398034e-08 9.592830e-07 5 6631873 6631931 59 - 2.413 2.256 -0.526
ENSG00000037474 E031 176.480900 0.0007367264 1.873424e-10 3.153956e-09 5 6631932 6631977 46 - 2.323 2.146 -0.594
ENSG00000037474 E032 342.119267 0.0031056024 3.696506e-10 5.928146e-09 5 6632599 6632756 158 - 2.618 2.418 -0.665
ENSG00000037474 E033 320.386777 0.0059594102 8.269443e-10 1.244733e-08 5 6632884 6633291 408 - 2.608 2.354 -0.848