ENSG00000036257

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000409096 ENSG00000036257 HEK293_OSMI2_2hA HEK293_TMG_2hB CUL3 protein_coding protein_coding 37.43697 24.93128 52.62019 1.282683 1.597537 1.077355 24.741525 13.072230 34.89806 1.6900620 1.6134001 1.415952 0.64707083 0.5237333 0.6626667 0.1389333 1.673951e-01 8.781078e-19 FALSE TRUE
ENST00000454323 ENSG00000036257 HEK293_OSMI2_2hA HEK293_TMG_2hB CUL3 protein_coding nonsense_mediated_decay 37.43697 24.93128 52.62019 1.282683 1.597537 1.077355 2.056705 7.177456 0.00000 2.2060983 0.0000000 -9.489337 0.08295833 0.2846000 0.0000000 -0.2846000 8.781078e-19 8.781078e-19   FALSE
MSTRG.20026.3 ENSG00000036257 HEK293_OSMI2_2hA HEK293_TMG_2hB CUL3 protein_coding   37.43697 24.93128 52.62019 1.282683 1.597537 1.077355 8.712219 3.771597 14.60306 0.7971807 0.5827218 1.950190 0.22008333 0.1552000 0.2780333 0.1228333 1.184668e-01 8.781078e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000036257 E001 99.1601490 0.0003179739 8.894234e-02 1.735078e-01 2 224470150 224473901 3752 - 1.949 2.019 0.236
ENSG00000036257 E002 12.7053597 0.0013386727 9.144658e-03 2.664454e-02 2 224473902 224473909 8 - 1.008 1.261 0.910
ENSG00000036257 E003 16.1674984 0.0011232585 9.083959e-05 4.790261e-04 2 224473910 224473921 12 - 1.066 1.404 1.194
ENSG00000036257 E004 18.4359196 0.0010670801 1.645901e-04 8.117754e-04 2 224473922 224473938 17 - 1.138 1.445 1.078
ENSG00000036257 E005 154.4904095 0.0063457609 5.635319e-14 1.659559e-12 2 224473939 224474032 94 - 2.007 2.375 1.232
ENSG00000036257 E006 490.2609693 0.0074523646 1.916678e-10 3.222193e-09 2 224474033 224474376 344 - 2.550 2.832 0.937
ENSG00000036257 E007 293.0094451 0.0001832918 1.305119e-16 5.535054e-15 2 224478200 224478302 103 - 2.369 2.554 0.618
ENSG00000036257 E008 209.9408256 0.0002358532 1.406730e-11 2.858530e-10 2 224478303 224478345 43 - 2.228 2.408 0.599
ENSG00000036257 E009 2.5473889 0.0110915802 2.401581e-01 3.743212e-01 2 224478346 224480124 1779 - 0.459 0.658 0.918
ENSG00000036257 E010 339.5645800 0.0017335632 1.413976e-08 1.695626e-07 2 224481892 224482078 187 - 2.444 2.606 0.540
ENSG00000036257 E011 0.2924217 0.0272695963 5.615193e-01   2 224485211 224485384 174 - 0.151 0.000 -9.572
ENSG00000036257 E012 1.9875256 0.0712780708 4.080807e-01 5.516598e-01 2 224495006 224495831 826 - 0.391 0.551 0.807
ENSG00000036257 E013 297.7958177 0.0018207266 6.295525e-02 1.316386e-01 2 224495832 224495966 135 - 2.425 2.487 0.207
ENSG00000036257 E014 309.1454279 0.0001566220 3.969743e-01 5.407439e-01 2 224497753 224497849 97 - 2.455 2.480 0.082
ENSG00000036257 E015 342.1980377 0.0001873631 1.860656e-01 3.088193e-01 2 224500363 224500487 125 - 2.515 2.494 -0.071
ENSG00000036257 E016 195.6345814 0.0002138933 3.161205e-01 4.588780e-01 2 224502965 224502991 27 - 2.273 2.252 -0.071
ENSG00000036257 E017 220.3850317 0.0002575176 7.135211e-01 8.100008e-01 2 224502992 224503072 81 - 2.318 2.315 -0.010
ENSG00000036257 E018 307.5799136 0.0016536456 1.064637e-01 2.003327e-01 2 224503652 224503822 171 - 2.474 2.434 -0.132
ENSG00000036257 E019 0.6266857 0.0168516475 3.938198e-01 5.377191e-01 2 224503823 224504324 502 - 0.152 0.304 1.280
ENSG00000036257 E020 0.2934659 0.0296353764 5.237285e-01   2 224505749 224505955 207 - 0.082 0.178 1.279
ENSG00000036257 E021 310.2657216 0.0036577736 2.686103e-01 4.070895e-01 2 224505956 224506132 177 - 2.477 2.444 -0.111
ENSG00000036257 E022 258.5220138 0.0015855151 3.335370e-02 7.848805e-02 2 224506858 224507003 146 - 2.403 2.346 -0.188
ENSG00000036257 E023 220.5742230 0.0091288613 4.384444e-02 9.810131e-02 2 224511354 224511437 84 - 2.344 2.256 -0.292
ENSG00000036257 E024 263.3250692 0.0038495551 2.247409e-03 7.990633e-03 2 224511438 224511582 145 - 2.424 2.321 -0.344
ENSG00000036257 E025 162.9945184 0.0007338358 3.388074e-04 1.535735e-03 2 224513524 224513614 91 - 2.219 2.108 -0.372
ENSG00000036257 E026 122.8323124 0.0090585581 3.474432e-03 1.165157e-02 2 224513615 224513638 24 - 2.109 1.952 -0.527
ENSG00000036257 E027 151.6756936 0.0012293611 8.189211e-07 6.928463e-06 2 224514612 224514635 24 - 2.204 2.031 -0.578
ENSG00000036257 E028 273.2400690 0.0120220244 4.274936e-05 2.448006e-04 2 224514636 224514772 137 - 2.471 2.254 -0.724
ENSG00000036257 E029 0.0000000       2 224532981 224533094 114 -      
ENSG00000036257 E030 207.6106613 0.0022616569 7.140763e-12 1.522733e-10 2 224535528 224535606 79 - 2.356 2.113 -0.812
ENSG00000036257 E031 147.2382919 0.0002377382 2.468058e-14 7.661253e-13 2 224535607 224535641 35 - 2.212 1.956 -0.857
ENSG00000036257 E032 2.7462632 0.0247085419 2.361183e-01 3.696760e-01 2 224556295 224556389 95 - 0.616 0.402 -1.043
ENSG00000036257 E033 1.3328661 0.0216249895 2.338832e-01 3.669946e-01 2 224556390 224556420 31 - 0.264 0.482 1.282
ENSG00000036257 E034 221.5671259 0.0008883073 3.694775e-16 1.477029e-14 2 224557659 224557856 198 - 2.386 2.141 -0.820
ENSG00000036257 E035 0.0000000       2 224560440 224560668 229 -      
ENSG00000036257 E036 0.1817044 0.0407488306 2.009807e-01   2 224562700 224563193 494 - 0.000 0.178 10.861
ENSG00000036257 E037 0.0000000       2 224563194 224563320 127 -      
ENSG00000036257 E038 0.0000000       2 224569689 224569825 137 -      
ENSG00000036257 E039 155.5948415 0.0125303757 3.193816e-06 2.374922e-05 2 224584944 224585397 454 - 2.244 1.954 -0.972