ENSG00000035664

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261891 ENSG00000035664 HEK293_OSMI2_2hA HEK293_TMG_2hB DAPK2 protein_coding protein_coding 0.5743507 0.7322441 0.7068313 0.05805879 0.1084632 -0.05026001 0.11410599 0.07676344 0.09694155 0.07676344 0.01619224 0.3016633 0.22294167 0.1076667 0.15220000 0.04453333 0.64062776 0.01844307 FALSE TRUE
ENST00000457488 ENSG00000035664 HEK293_OSMI2_2hA HEK293_TMG_2hB DAPK2 protein_coding protein_coding 0.5743507 0.7322441 0.7068313 0.05805879 0.1084632 -0.05026001 0.16240874 0.49227702 0.06995770 0.09595243 0.06995770 -2.6511745 0.29760417 0.6668000 0.08863333 -0.57816667 0.01844307 0.01844307 FALSE TRUE
ENST00000558064 ENSG00000035664 HEK293_OSMI2_2hA HEK293_TMG_2hB DAPK2 protein_coding nonsense_mediated_decay 0.5743507 0.7322441 0.7068313 0.05805879 0.1084632 -0.05026001 0.06652685 0.00000000 0.06006345 0.00000000 0.06006345 2.8086619 0.08981667 0.0000000 0.07610000 0.07610000 0.76874274 0.01844307   FALSE
ENST00000558482 ENSG00000035664 HEK293_OSMI2_2hA HEK293_TMG_2hB DAPK2 protein_coding processed_transcript 0.5743507 0.7322441 0.7068313 0.05805879 0.1084632 -0.05026001 0.04153277 0.03876995 0.11234065 0.02066248 0.08724580 1.3268393 0.07022083 0.0511000 0.15580000 0.10470000 0.79176269 0.01844307 FALSE TRUE
ENST00000559007 ENSG00000035664 HEK293_OSMI2_2hA HEK293_TMG_2hB DAPK2 protein_coding nonsense_mediated_decay 0.5743507 0.7322441 0.7068313 0.05805879 0.1084632 -0.05026001 0.07393840 0.11369259 0.04279229 0.02282174 0.01469656 -1.2283599 0.14457917 0.1589667 0.06473333 -0.09423333 0.40754022 0.01844307 TRUE TRUE
ENST00000559731 ENSG00000035664 HEK293_OSMI2_2hA HEK293_TMG_2hB DAPK2 protein_coding processed_transcript 0.5743507 0.7322441 0.7068313 0.05805879 0.1084632 -0.05026001 0.04840856 0.00000000 0.13955412 0.00000000 0.07315738 3.9025958 0.06628750 0.0000000 0.23570000 0.23570000 0.26067159 0.01844307   FALSE
ENST00000561162 ENSG00000035664 HEK293_OSMI2_2hA HEK293_TMG_2hB DAPK2 protein_coding nonsense_mediated_decay 0.5743507 0.7322441 0.7068313 0.05805879 0.1084632 -0.05026001 0.01663983 0.00000000 0.13311865 0.00000000 0.13311865 3.8391398 0.01982500 0.0000000 0.15860000 0.15860000 0.80367130 0.01844307 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000035664 E001 5.0707006 0.055238255 0.743308944 0.831965506 15 63907036 63907922 887 - 0.784 0.730 -0.215
ENSG00000035664 E002 9.5216068 0.002928216 0.002526722 0.008842827 15 63907923 63908252 330 - 0.712 1.071 1.372
ENSG00000035664 E003 8.5880942 0.010207681 0.064322638 0.133902534 15 63908253 63908600 348 - 0.784 1.014 0.878
ENSG00000035664 E004 2.7324121 0.049887929 0.587141578 0.710486077 15 63908601 63909183 583 - 0.455 0.551 0.465
ENSG00000035664 E005 0.2998086 0.028798201 0.609399218   15 63909184 63909214 31 - 0.165 0.089 -1.014
ENSG00000035664 E006 1.1176488 0.134251631 0.854865817 0.910224927 15 63909215 63909541 327 - 0.285 0.328 0.282
ENSG00000035664 E007 1.3275399 0.255265580 0.379026374 0.523321086 15 63909677 63909774 98 - 0.165 0.384 1.620
ENSG00000035664 E008 0.3332198 0.029852439 0.614101970   15 63910623 63910699 77 - 0.165 0.090 -1.011
ENSG00000035664 E009 0.5169874 0.020594962 1.000000000 1.000000000 15 63910700 63911216 517 - 0.165 0.164 -0.011
ENSG00000035664 E010 0.9598924 0.018352040 0.397262616 0.541024793 15 63911217 63911851 635 - 0.378 0.227 -1.012
ENSG00000035664 E011 0.1451727 0.052826496 0.246410588   15 63911852 63911907 56 - 0.165 0.000 -13.548
ENSG00000035664 E012 2.5839456 0.006232985 0.719859340 0.814588763 15 63911908 63911943 36 - 0.577 0.517 -0.276
ENSG00000035664 E013 2.8731451 0.005706867 0.611055820 0.730253413 15 63911944 63911991 48 - 0.627 0.546 -0.361
ENSG00000035664 E014 3.6075148 0.004938215 0.038988093 0.089173211 15 63912108 63912197 90 - 0.816 0.518 -1.273
ENSG00000035664 E015 0.1515154 0.043167566 0.247247178   15 63916147 63916575 429 - 0.165 0.000 -13.558
ENSG00000035664 E016 0.3030308 0.234489296 0.105318944   15 63916576 63916592 17 - 0.285 0.000 -14.202
ENSG00000035664 E017 0.8178402 0.017849070 0.522436862 0.656250017 15 63916593 63922734 6142 - 0.165 0.282 0.986
ENSG00000035664 E018 0.3268771 0.032574491 0.611228145   15 63922735 63923343 609 - 0.165 0.090 -1.010
ENSG00000035664 E019 0.0000000       15 63924512 63924815 304 -      
ENSG00000035664 E020 2.7991222 0.014646278 0.735162944 0.825975959 15 63924816 63924861 46 - 0.577 0.518 -0.270
ENSG00000035664 E021 4.1254421 0.004557949 0.373209167 0.517649457 15 63925941 63926074 134 - 0.577 0.710 0.574
ENSG00000035664 E022 2.3958960 0.006624939 0.105249313 0.198472737 15 63926075 63926093 19 - 0.284 0.574 1.573
ENSG00000035664 E023 0.3299976 0.027442404 0.466562891   15 63926094 63926201 108 - 0.000 0.164 12.120
ENSG00000035664 E024 2.1421709 0.008133366 0.040320773 0.091621282 15 63929551 63929577 27 - 0.165 0.547 2.448
ENSG00000035664 E025 0.0000000       15 63929674 63929778 105 -      
ENSG00000035664 E026 1.8423623 0.008482015 0.008317427 0.024585536 15 63930407 63930455 49 - 0.000 0.518 13.957
ENSG00000035664 E027 1.5403913 0.012449317 0.123750100 0.225296084 15 63939232 63939292 61 - 0.165 0.452 1.988
ENSG00000035664 E028 1.3221683 0.110193474 0.649533402 0.760792121 15 63939293 63939361 69 - 0.284 0.374 0.564
ENSG00000035664 E029 2.7730118 0.006014614 0.496021895 0.632755321 15 63971423 63971523 101 - 0.627 0.517 -0.498
ENSG00000035664 E030 2.1882570 0.007665771 0.696367078 0.796735452 15 63971524 63971561 38 - 0.520 0.452 -0.333
ENSG00000035664 E031 2.7523747 0.108599302 0.189362532 0.312931582 15 63983533 63983604 72 - 0.713 0.451 -1.194
ENSG00000035664 E032 2.0860500 0.042046118 0.014881098 0.040223997 15 63983605 63983624 20 - 0.713 0.283 -2.181
ENSG00000035664 E033 3.1083966 0.005652889 0.081839505 0.162443409 15 63983625 63983754 130 - 0.750 0.486 -1.163
ENSG00000035664 E034 0.0000000       15 63991228 63991351 124 -      
ENSG00000035664 E035 0.0000000       15 64029789 64030020 232 -      
ENSG00000035664 E036 1.8422657 0.010864776 0.232693043 0.365648915 15 64040170 64040267 98 - 0.577 0.375 -1.014
ENSG00000035664 E037 1.1028881 0.012106076 0.198893222 0.324819608 15 64046298 64046322 25 - 0.455 0.227 -1.427
ENSG00000035664 E038 0.9211837 0.013599154 0.096974842 0.185862642 15 64046323 64046381 59 - 0.455 0.164 -2.012
ENSG00000035664 E039 0.1515154 0.043167566 0.247247178   15 64046382 64046436 55 - 0.165 0.000 -13.558
ENSG00000035664 E040 0.0000000       15 64063160 64063318 159 -      
ENSG00000035664 E041 0.1472490 0.043628601 0.247317265   15 64064236 64064447 212 - 0.165 0.000 -13.558
ENSG00000035664 E042 0.0000000       15 64071929 64072033 105 -