ENSG00000035499

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000265036 ENSG00000035499 HEK293_OSMI2_2hA HEK293_TMG_2hB DEPDC1B protein_coding protein_coding 15.60597 5.379692 27.91914 0.7624572 1.028604 2.373496 11.479405 5.0879253 19.36853 0.81020102 1.2473913 1.926477 0.8173292 0.94136667 0.6922667 -0.2491000 1.622488e-07 1.622488e-07 FALSE TRUE
ENST00000453022 ENSG00000035499 HEK293_OSMI2_2hA HEK293_TMG_2hB DEPDC1B protein_coding protein_coding 15.60597 5.379692 27.91914 0.7624572 1.028604 2.373496 3.098189 0.2073366 6.25740 0.07775889 0.9315975 4.849864 0.1365792 0.04273333 0.2240000 0.1812667 9.488906e-04 1.622488e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000035499 E001 108.4329864 0.0004928004 3.369120e-20 2.198655e-18 5 60596912 60597269 358 - 1.867 2.209 1.146
ENSG00000035499 E002 84.7305364 0.0007099942 2.667748e-21 1.974978e-19 5 60597270 60597442 173 - 1.739 2.135 1.330
ENSG00000035499 E003 87.7242033 0.0122969528 1.266927e-05 8.221682e-05 5 60597443 60597628 186 - 1.787 2.090 1.020
ENSG00000035499 E004 87.3986954 0.0003427589 1.159049e-04 5.951278e-04 5 60597629 60597667 39 - 1.829 2.001 0.576
ENSG00000035499 E005 193.9712398 0.0002197278 3.437098e-01 4.877658e-01 5 60597668 60597908 241 - 2.209 2.247 0.125
ENSG00000035499 E006 61.5445983 0.0004116921 2.145983e-01 3.439742e-01 5 60597909 60597914 6 - 1.707 1.781 0.249
ENSG00000035499 E007 146.6648605 0.0008168766 2.162254e-01 3.458659e-01 5 60599075 60599260 186 - 2.105 2.069 -0.121
ENSG00000035499 E008 149.2941662 0.0008333309 7.526271e-03 2.258096e-02 5 60603391 60603567 177 - 2.123 2.031 -0.309
ENSG00000035499 E009 0.0000000       5 60605684 60605689 6 -      
ENSG00000035499 E010 142.3929070 0.0002271060 1.315708e-01 2.363253e-01 5 60605690 60605856 167 - 2.095 2.051 -0.146
ENSG00000035499 E011 2.0066570 0.0079092713 6.526999e-01 7.633690e-01 5 60628214 60628691 478 - 0.445 0.363 -0.451
ENSG00000035499 E012 138.0465043 0.0002400192 6.335250e-01 7.482181e-01 5 60638750 60638890 141 - 2.074 2.066 -0.025
ENSG00000035499 E013 79.0894549 0.0013714991 9.635259e-01 9.810077e-01 5 60642812 60642859 48 - 1.832 1.838 0.020
ENSG00000035499 E014 115.0739119 0.0002728569 2.269398e-03 8.057321e-03 5 60644745 60644875 131 - 2.018 1.903 -0.386
ENSG00000035499 E015 56.3870424 0.0004195010 6.199004e-03 1.913891e-02 5 60645492 60645515 24 - 1.720 1.567 -0.519
ENSG00000035499 E016 119.6576976 0.0002614541 4.030430e-08 4.429218e-07 5 60645516 60645619 104 - 2.052 1.830 -0.746
ENSG00000035499 E017 124.9847255 0.0002722931 5.402435e-08 5.781365e-07 5 60647398 60647533 136 - 2.069 1.854 -0.721
ENSG00000035499 E018 0.0000000       5 60647534 60648147 614 -      
ENSG00000035499 E019 145.9886876 0.0002725598 2.821790e-08 3.193366e-07 5 60686962 60687227 266 - 2.134 1.934 -0.672
ENSG00000035499 E020 1.8217435 0.0297639621 7.586854e-01 8.432127e-01 5 60687725 60687838 114 - 0.419 0.362 -0.317
ENSG00000035499 E021 0.2924217 0.0275957316 1.000000e+00   5 60688985 60689050 66 - 0.122 0.000 -8.175
ENSG00000035499 E022 44.5173199 0.0067756342 4.562363e-05 2.596119e-04 5 60700046 60700190 145 - 1.645 1.313 -1.143