ENSG00000035403

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000372755 ENSG00000035403 HEK293_OSMI2_2hA HEK293_TMG_2hB VCL protein_coding protein_coding 42.59755 34.27433 51.52354 3.618709 0.7758257 0.5879624 31.949882 33.2947674 32.85919 3.1747256 0.2315607 -0.01899301 0.7790083 0.9734333 0.6379000 -0.3355333 1.749751e-12 1.749751e-12 FALSE TRUE
MSTRG.4209.2 ENSG00000035403 HEK293_OSMI2_2hA HEK293_TMG_2hB VCL protein_coding   42.59755 34.27433 51.52354 3.618709 0.7758257 0.5879624 9.783737 0.7861971 17.90501 0.5515415 0.7417932 4.49189904 0.1975750 0.0204000 0.3472667 0.3268667 1.137402e-06 1.749751e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000035403 E001 0.0000000       10 73995193 73995231 39 +      
ENSG00000035403 E002 0.0000000       10 73997182 73997221 40 +      
ENSG00000035403 E003 9.3174806 0.0195902471 2.398122e-01 3.739143e-01 10 73998104 73998113 10 + 1.046 0.978 -0.250
ENSG00000035403 E004 9.4689960 0.0173905134 1.995055e-01 3.256174e-01 10 73998114 73998115 2 + 1.057 0.978 -0.288
ENSG00000035403 E005 250.0915692 0.0046583968 3.090083e-07 2.851471e-06 10 73998116 73998375 260 + 2.453 2.328 -0.416
ENSG00000035403 E006 0.0000000       10 74016832 74016878 47 +      
ENSG00000035403 E007 77.4928615 0.0007133698 3.272057e-04 1.489527e-03 10 74043083 74043090 8 + 1.928 1.856 -0.240
ENSG00000035403 E008 150.3321935 0.0002953149 1.383803e-11 2.817382e-10 10 74043091 74043153 63 + 2.233 2.110 -0.412
ENSG00000035403 E009 0.3666179 0.0333675388 8.990665e-01 9.396126e-01 10 74063686 74063890 205 + 0.105 0.142 0.506
ENSG00000035403 E010 264.8519707 0.0019105368 4.500438e-10 7.108508e-09 10 74070670 74070820 151 + 2.472 2.361 -0.370
ENSG00000035403 E011 246.2086554 0.0007859200 2.122986e-06 1.641740e-05 10 74070975 74071083 109 + 2.413 2.368 -0.152
ENSG00000035403 E012 263.4424623 0.0050176001 1.576294e-02 4.220062e-02 10 74072730 74072852 123 + 2.430 2.412 -0.061
ENSG00000035403 E013 355.7512693 0.0054434328 1.917096e-04 9.283710e-04 10 74074743 74074903 161 + 2.582 2.513 -0.230
ENSG00000035403 E014 0.8899506 0.0131838045 1.360238e-02 3.731153e-02 10 74074904 74077748 2845 + 0.422 0.000 -10.890
ENSG00000035403 E015 316.8076820 0.0014483969 4.101783e-12 9.093862e-11 10 74082454 74082544 91 + 2.549 2.439 -0.365
ENSG00000035403 E016 386.7426138 0.0019429544 2.411499e-12 5.559984e-11 10 74083366 74083513 148 + 2.638 2.523 -0.384
ENSG00000035403 E017 358.1581076 0.0036881818 1.542868e-05 9.818991e-05 10 74089196 74089349 154 + 2.585 2.516 -0.231
ENSG00000035403 E018 405.4305854 0.0025670134 1.310564e-06 1.061218e-05 10 74090023 74090198 176 + 2.637 2.571 -0.222
ENSG00000035403 E019 445.0620994 0.0001929168 9.818126e-14 2.793372e-12 10 74094271 74094461 191 + 2.672 2.619 -0.177
ENSG00000035403 E020 410.2113612 0.0008923966 1.320346e-04 6.677046e-04 10 74095656 74095855 200 + 2.617 2.610 -0.023
ENSG00000035403 E021 311.3819570 0.0002737930 1.967030e-06 1.532459e-05 10 74097204 74097332 129 + 2.506 2.484 -0.073
ENSG00000035403 E022 328.8321755 0.0002102459 6.428596e-04 2.690314e-03 10 74100948 74101053 106 + 2.515 2.524 0.032
ENSG00000035403 E023 220.3563274 0.0002023893 2.105194e-02 5.370265e-02 10 74101054 74101097 44 + 2.336 2.358 0.076
ENSG00000035403 E024 324.6832201 0.0001976648 7.371693e-03 2.218393e-02 10 74103820 74103928 109 + 2.503 2.527 0.082
ENSG00000035403 E025 1.9617168 0.0104439735 3.907345e-01 5.346972e-01 10 74104928 74105050 123 + 0.503 0.407 -0.496
ENSG00000035403 E026 608.8236715 0.0008889854 8.645372e-03 2.539874e-02 10 74105051 74105353 303 + 2.773 2.799 0.085
ENSG00000035403 E027 360.1004843 0.0001743731 6.475523e-03 1.986059e-02 10 74107230 74107354 125 + 2.546 2.573 0.088
ENSG00000035403 E028 447.8795496 0.0001457179 9.349701e-01 9.630669e-01 10 74108971 74109156 186 + 2.616 2.694 0.257
ENSG00000035403 E029 5.1855470 0.0247252710 8.916686e-01 9.348015e-01 10 74111362 74111523 162 + 0.752 0.833 0.323
ENSG00000035403 E030 9.0735988 0.0546579148 7.758078e-02 1.555763e-01 10 74111909 74112112 204 + 1.086 0.879 -0.769
ENSG00000035403 E031 477.6070952 0.0005843247 2.739692e-01 4.130638e-01 10 74114184 74114387 204 + 2.632 2.732 0.333
ENSG00000035403 E032 354.7670891 0.0001585958 1.454379e-01 2.554495e-01 10 74114795 74114899 105 + 2.498 2.605 0.355
ENSG00000035403 E033 3115.3629009 0.0071321121 2.090687e-14 6.561869e-13 10 74118023 74121363 3341 + 3.325 3.631 1.016