ENSG00000034677

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000341084 ENSG00000034677 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF19A protein_coding protein_coding 5.627597 1.103747 10.30268 0.04850546 0.8352335 3.210926 3.9885210 0.9990026 6.855128 0.01281405 0.3078538 2.766357 0.7774667 0.9077 0.6783000 -0.2294000 0.097328983 0.001048708 FALSE TRUE
ENST00000520903 ENSG00000034677 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF19A protein_coding retained_intron 5.627597 1.103747 10.30268 0.04850546 0.8352335 3.210926 0.9399154 0.0000000 1.861919 0.00000000 0.5855514 7.548374 0.1043708 0.0000 0.1740333 0.1740333 0.001048708 0.001048708 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000034677 E001 0.5890081 0.0299290082 5.622625e-02 1.201020e-01 8 100237293 100237434 142 - 0.082 0.482 3.295
ENSG00000034677 E002 0.1472490 0.0426812495 7.060773e-01   8 100257060 100257066 7 - 0.043 0.000 -7.696
ENSG00000034677 E003 182.6640907 0.0244114415 4.683100e-07 4.166428e-06 8 100257067 100258884 1818 - 1.992 2.402 1.369
ENSG00000034677 E004 43.5557185 0.0005855117 2.309428e-02 5.794276e-02 8 100258885 100259062 178 - 1.456 1.351 -0.362
ENSG00000034677 E005 29.3047365 0.0711333884 2.396315e-01 3.736743e-01 8 100259063 100259106 44 - 1.292 1.219 -0.255
ENSG00000034677 E006 30.9912945 0.0007047024 4.996960e-01 6.359733e-01 8 100259107 100259163 57 - 1.303 1.331 0.099
ENSG00000034677 E007 38.8629223 0.0005497090 5.668242e-01 6.937030e-01 8 100259164 100259246 83 - 1.395 1.440 0.155
ENSG00000034677 E008 42.5204194 0.0005258031 1.282714e-01 2.317370e-01 8 100259854 100259997 144 - 1.442 1.407 -0.121
ENSG00000034677 E009 0.0000000       8 100259998 100260040 43 -      
ENSG00000034677 E010 51.0497802 0.0004681569 4.205941e-02 9.483716e-02 8 100261542 100261722 181 - 1.522 1.456 -0.226
ENSG00000034677 E011 25.1774462 0.0008162166 1.734957e-02 4.571128e-02 8 100261723 100261755 33 - 1.240 1.050 -0.682
ENSG00000034677 E012 43.9000145 0.0004810543 1.851863e-02 4.827115e-02 8 100264034 100264195 162 - 1.462 1.351 -0.384
ENSG00000034677 E013 1.2565685 0.0108647759 9.221561e-01 9.548989e-01 8 100264196 100264580 385 - 0.237 0.306 0.494
ENSG00000034677 E014 30.3002310 0.0009305018 6.979676e-02 1.430356e-01 8 100264671 100264785 115 - 1.307 1.213 -0.332
ENSG00000034677 E015 0.0000000       8 100268604 100268784 181 -      
ENSG00000034677 E016 35.2206203 0.0006431028 1.749140e-01 2.945663e-01 8 100268785 100268947 163 - 1.363 1.331 -0.113
ENSG00000034677 E017 29.6691501 0.0006623187 3.406264e-01 4.845807e-01 8 100269869 100270013 145 - 1.289 1.287 -0.006
ENSG00000034677 E018 30.5208315 0.0006912018 1.720447e-01 2.908614e-01 8 100274953 100275161 209 - 1.307 1.264 -0.152
ENSG00000034677 E019 33.6027138 0.0010481661 8.141193e-01 8.822166e-01 8 100287501 100287677 177 - 1.335 1.407 0.248
ENSG00000034677 E020 17.1543609 0.0010839498 9.610084e-01 9.794599e-01 8 100287678 100287704 27 - 1.060 1.155 0.342
ENSG00000034677 E021 27.5260746 0.0007335449 9.024867e-01 9.418918e-01 8 100287705 100287834 130 - 1.248 1.351 0.361
ENSG00000034677 E022 18.7498110 0.0095753529 4.260830e-01 5.689559e-01 8 100287835 100287906 72 - 1.104 1.088 -0.056
ENSG00000034677 E023 34.0818685 0.0006219468 7.917573e-03 2.357101e-02 8 100287907 100288091 185 - 1.363 1.185 -0.627
ENSG00000034677 E024 15.0058432 0.0011443631 7.020262e-02 1.436950e-01 8 100288092 100288130 39 - 1.031 0.853 -0.670
ENSG00000034677 E025 15.8831309 0.0010726213 7.981840e-01 8.711936e-01 8 100288131 100288206 76 - 1.031 1.088 0.205
ENSG00000034677 E026 14.5531578 0.0035863492 3.326703e-01 4.763457e-01 8 100288207 100288267 61 - 1.010 0.962 -0.176
ENSG00000034677 E027 0.1472490 0.0426812495 7.060773e-01   8 100300528 100300542 15 - 0.043 0.000 -7.696
ENSG00000034677 E028 0.1472490 0.0426812495 7.060773e-01   8 100300543 100300577 35 - 0.043 0.000 -7.696
ENSG00000034677 E029 0.2924217 0.0290785164 1.000000e+00   8 100300578 100300651 74 - 0.082 0.000 -8.695
ENSG00000034677 E030 1.6543084 0.1788131848 9.106595e-01 9.472137e-01 8 100303160 100303365 206 - 0.309 0.306 -0.016
ENSG00000034677 E031 0.4417471 0.6870626855 1.000000e+00 1.000000e+00 8 100303366 100303401 36 - 0.117 0.000 -9.278
ENSG00000034677 E032 0.5869198 0.1590780422 1.000000e+00 1.000000e+00 8 100309298 100309526 229 - 0.151 0.000 -9.694
ENSG00000034677 E033 12.9212136 0.0013385720 1.798465e-01 3.009892e-01 8 100309867 100310148 282 - 0.968 0.853 -0.437
ENSG00000034677 E034 0.0000000       8 100312344 100312410 67 -      
ENSG00000034677 E035 0.0000000       8 100313291 100313372 82 -      
ENSG00000034677 E036 0.0000000       8 100336108 100336218 111 -