ENSG00000033178

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000322244 ENSG00000033178 HEK293_OSMI2_2hA HEK293_TMG_2hB UBA6 protein_coding protein_coding 19.34573 1.645654 35.99159 0.1703786 0.7968336 4.442588 1.714875 0.7871951 3.001183 0.1098570 0.09825505 1.917325 0.2151375 0.4841333 0.08343333 -0.4007000 6.484538e-11 3.896188e-23 FALSE TRUE
ENST00000506571 ENSG00000033178 HEK293_OSMI2_2hA HEK293_TMG_2hB UBA6 protein_coding retained_intron 19.34573 1.645654 35.99159 0.1703786 0.7968336 4.442588 7.747559 0.0000000 15.894485 0.0000000 0.75496284 10.635218 0.2230708 0.0000000 0.44123333 0.4412333 3.896188e-23 3.896188e-23 FALSE TRUE
MSTRG.24971.2 ENSG00000033178 HEK293_OSMI2_2hA HEK293_TMG_2hB UBA6 protein_coding   19.34573 1.645654 35.99159 0.1703786 0.7968336 4.442588 5.965851 0.7981909 10.345976 0.1897052 0.85710652 3.679624 0.4221500 0.4775333 0.28820000 -0.1893333 1.563046e-01 3.896188e-23 FALSE TRUE
MSTRG.24971.3 ENSG00000033178 HEK293_OSMI2_2hA HEK293_TMG_2hB UBA6 protein_coding   19.34573 1.645654 35.99159 0.1703786 0.7968336 4.442588 3.589264 0.0000000 6.322464 0.0000000 0.69336107 9.306623 0.0984750 0.0000000 0.17550000 0.1755000 5.323919e-08 3.896188e-23 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000033178 E001 209.6339452 0.0255650920 3.467867e-11 6.606104e-10 4 67612652 67617446 4795 - 1.942 2.615 2.248
ENSG00000033178 E002 334.0865753 0.0191107668 4.408953e-10 6.974474e-09 4 67617447 67618668 1222 - 2.173 2.723 1.836
ENSG00000033178 E003 87.8652418 0.0015567408 8.574452e-01 9.119668e-01 4 67618669 67618854 186 - 1.662 1.804 0.481
ENSG00000033178 E004 132.5390324 0.0002230787 9.411745e-01 9.670129e-01 4 67618855 67619132 278 - 1.838 1.989 0.509
ENSG00000033178 E005 81.4829577 0.0003197904 7.138863e-01 8.102570e-01 4 67622831 67622925 95 - 1.627 1.804 0.600
ENSG00000033178 E006 71.1856022 0.0003960137 7.970503e-01 8.704977e-01 4 67623135 67623222 88 - 1.573 1.709 0.460
ENSG00000033178 E007 80.0445085 0.0022160650 5.646034e-01 6.918501e-01 4 67624126 67624253 128 - 1.627 1.740 0.383
ENSG00000033178 E008 94.1101069 0.0003265480 1.772892e-01 2.977062e-01 4 67624994 67625187 194 - 1.699 1.768 0.233
ENSG00000033178 E009 80.5899494 0.0006471491 1.508833e-01 2.628667e-01 4 67626360 67626477 118 - 1.633 1.687 0.183
ENSG00000033178 E010 56.6583269 0.0012855180 1.866537e-01 3.095351e-01 4 67629071 67629142 72 - 1.484 1.526 0.143
ENSG00000033178 E011 0.1515154 0.0430723825 5.643682e-01   4 67630454 67630465 12 - 0.035 0.000 -7.264
ENSG00000033178 E012 50.1339562 0.0007860678 4.616518e-03 1.486581e-02 4 67630466 67630535 70 - 1.447 1.323 -0.428
ENSG00000033178 E013 47.3632788 0.0004943815 2.356278e-03 8.327545e-03 4 67631708 67631771 64 - 1.425 1.268 -0.547
ENSG00000033178 E014 43.3988250 0.0004702276 9.033383e-03 2.636208e-02 4 67631857 67631908 52 - 1.386 1.268 -0.412
ENSG00000033178 E015 60.9478219 0.0004085301 2.358112e-04 1.115839e-03 4 67633345 67633473 129 - 1.532 1.348 -0.634
ENSG00000033178 E016 53.4478538 0.0041474267 1.034020e-02 2.960525e-02 4 67634242 67634322 81 - 1.472 1.372 -0.344
ENSG00000033178 E017 62.8713639 0.0016569218 1.210461e-02 3.378687e-02 4 67634429 67634518 90 - 1.536 1.475 -0.211
ENSG00000033178 E018 69.0826344 0.0005258999 6.002442e-03 1.861654e-02 4 67635453 67635558 106 - 1.577 1.510 -0.231
ENSG00000033178 E019 88.6724048 0.0003125119 9.332130e-04 3.730391e-03 4 67638943 67639124 182 - 1.684 1.600 -0.283
ENSG00000033178 E020 52.5619913 0.0004611787 1.631073e-02 4.341327e-02 4 67641151 67641228 78 - 1.462 1.395 -0.233
ENSG00000033178 E021 47.9941821 0.0006538487 2.146426e-01 3.440241e-01 4 67644698 67644778 81 - 1.415 1.456 0.140
ENSG00000033178 E022 45.8180006 0.0025453843 1.070343e-01 2.011599e-01 4 67645938 67646016 79 - 1.399 1.395 -0.015
ENSG00000033178 E023 41.1530459 0.0010821324 1.104480e-01 2.061950e-01 4 67646724 67646791 68 - 1.356 1.348 -0.028
ENSG00000033178 E024 58.1465544 0.0144969197 4.210179e-02 9.491126e-02 4 67649068 67649211 144 - 1.505 1.439 -0.227
ENSG00000033178 E025 0.6224193 0.0182655989 3.170244e-02 7.527080e-02 4 67661663 67662188 526 - 0.068 0.544 3.888
ENSG00000033178 E026 43.4256529 0.0005065008 9.052397e-03 2.640862e-02 4 67662189 67662255 67 - 1.386 1.268 -0.412
ENSG00000033178 E027 0.8837216 0.1385789613 1.000000e+00 1.000000e+00 4 67662256 67662261 6 - 0.178 0.000 -9.848
ENSG00000033178 E028 2.5111744 0.0285604540 6.282840e-01 7.438507e-01 4 67662487 67663138 652 - 0.339 0.546 1.088
ENSG00000033178 E029 51.0438178 0.0060435776 1.024923e-01 1.943056e-01 4 67663139 67663215 77 - 1.444 1.438 -0.022
ENSG00000033178 E030 1.7757486 0.0076680965 2.951341e-01 4.362557e-01 4 67663216 67663884 669 - 0.304 0.000 -10.847
ENSG00000033178 E031 51.5631011 0.0015470370 7.017959e-03 2.127046e-02 4 67663885 67663947 63 - 1.456 1.349 -0.371
ENSG00000033178 E032 63.0320748 0.0003907582 6.096689e-04 2.568320e-03 4 67665189 67665292 104 - 1.544 1.395 -0.514
ENSG00000033178 E033 80.6746412 0.0003355111 8.015770e-07 6.794772e-06 4 67668551 67668674 124 - 1.654 1.394 -0.891
ENSG00000033178 E034 88.9261598 0.0003020194 6.721960e-10 1.029887e-08 4 67670470 67670592 123 - 1.700 1.323 -1.293
ENSG00000033178 E035 68.5312113 0.0003498152 3.648540e-05 2.123126e-04 4 67673697 67673777 81 - 1.582 1.372 -0.722
ENSG00000033178 E036 73.3554295 0.0003332039 3.161947e-03 1.074343e-02 4 67677611 67677722 112 - 1.604 1.526 -0.265
ENSG00000033178 E037 0.4375944 0.0257113437 1.000000e+00 1.000000e+00 4 67678430 67678438 9 - 0.098 0.000 -8.847
ENSG00000033178 E038 62.3441945 0.0003734188 7.706436e-06 5.254305e-05 4 67678439 67678533 95 - 1.545 1.268 -0.958
ENSG00000033178 E039 42.5541709 0.0005469066 8.623121e-06 5.814851e-05 4 67681563 67681591 29 - 1.391 0.990 -1.428
ENSG00000033178 E040 57.6642599 0.0003799611 7.396938e-04 3.041736e-03 4 67682119 67682213 95 - 1.508 1.348 -0.551
ENSG00000033178 E041 40.4530029 0.0005175652 8.465260e-05 4.500654e-04 4 67696645 67696707 63 - 1.368 1.042 -1.155
ENSG00000033178 E042 37.6464861 0.0005596347 2.195173e-05 1.347985e-04 4 67701049 67701155 107 - 1.340 0.930 -1.474