ENSG00000032219

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355431 ENSG00000032219 HEK293_OSMI2_2hA HEK293_TMG_2hB ARID4A protein_coding protein_coding 3.384068 0.8664863 5.54271 0.1366507 0.3350789 2.663389 2.2319136 0.5394702 3.28054648 0.1028270 0.20189805 2.582214 0.73276667 0.6561333 0.5924667 -0.06366667 0.879368381 0.001753674 FALSE TRUE
ENST00000417477 ENSG00000032219 HEK293_OSMI2_2hA HEK293_TMG_2hB ARID4A protein_coding protein_coding 3.384068 0.8664863 5.54271 0.1366507 0.3350789 2.663389 0.2635081 0.0000000 0.61943607 0.0000000 0.01910985 5.975988 0.04632500 0.0000000 0.1126667 0.11266667 0.001753674 0.001753674 FALSE TRUE
ENST00000466065 ENSG00000032219 HEK293_OSMI2_2hA HEK293_TMG_2hB ARID4A protein_coding retained_intron 3.384068 0.8664863 5.54271 0.1366507 0.3350789 2.663389 0.0569414 0.2766318 0.01065383 0.1419263 0.01065383 -3.794717 0.05155417 0.2965000 0.0020000 -0.29450000 0.020333446 0.001753674 FALSE FALSE
ENST00000469635 ENSG00000032219 HEK293_OSMI2_2hA HEK293_TMG_2hB ARID4A protein_coding retained_intron 3.384068 0.8664863 5.54271 0.1366507 0.3350789 2.663389 0.1553232 0.0000000 0.57919570 0.0000000 0.16822740 5.880675 0.03637917 0.0000000 0.1037000 0.10370000 0.028184522 0.001753674 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000032219 E001 0.4375944 0.0239974745 1.000000e+00 1.000000e+00 14 58298504 58298554 51 + 0.138 0.000 -8.652
ENSG00000032219 E002 0.5891098 0.0182041293 6.950093e-01 7.957076e-01 14 58298555 58298555 1 + 0.176 0.000 -11.048
ENSG00000032219 E003 0.5891098 0.0182041293 6.950093e-01 7.957076e-01 14 58298556 58298558 3 + 0.176 0.000 -11.048
ENSG00000032219 E004 2.8220326 0.0490021733 2.477316e-01 3.832656e-01 14 58298559 58298587 29 + 0.512 0.267 -1.407
ENSG00000032219 E005 8.3097619 0.0207671812 3.008735e-01 4.426498e-01 14 58298588 58298700 113 + 0.860 0.720 -0.558
ENSG00000032219 E006 0.5911862 0.0193874923 6.931876e-01 7.942513e-01 14 58299331 58299536 206 + 0.176 0.000 -11.045
ENSG00000032219 E007 0.8973375 0.1027690364 8.621771e-01 9.151293e-01 14 58299537 58299691 155 + 0.211 0.265 0.426
ENSG00000032219 E008 0.5901540 0.0198236789 5.440447e-01 6.745485e-01 14 58299692 58299797 106 + 0.138 0.266 1.172
ENSG00000032219 E009 11.5752938 0.0015118561 3.441600e-02 8.055583e-02 14 58299798 58299860 63 + 1.000 0.719 -1.089
ENSG00000032219 E010 16.7393958 0.0011347186 2.682928e-03 9.322513e-03 14 58301580 58301690 111 + 1.154 0.784 -1.384
ENSG00000032219 E011 0.2924217 0.0290785164 1.000000e+00   14 58303538 58303761 224 + 0.097 0.000 -10.132
ENSG00000032219 E012 12.8026194 0.0016199046 9.217620e-04 3.691237e-03 14 58304944 58305009 66 + 1.056 0.549 -2.031
ENSG00000032219 E013 15.5261546 0.0012194986 2.107380e-03 7.556724e-03 14 58306022 58306112 91 + 1.122 0.719 -1.533
ENSG00000032219 E014 18.9146051 0.0011116760 6.853605e-02 1.409399e-01 14 58318542 58318621 80 + 1.184 1.013 -0.614
ENSG00000032219 E015 19.7082188 0.0010961934 4.046038e-01 5.483168e-01 14 58318711 58318805 95 + 1.183 1.137 -0.166
ENSG00000032219 E016 18.0887071 0.0058292965 1.969050e-01 3.223578e-01 14 58323485 58323617 133 + 1.161 1.048 -0.410
ENSG00000032219 E017 13.0333634 0.0103838792 1.793027e-02 4.698611e-02 14 58328237 58328316 80 + 1.051 0.719 -1.274
ENSG00000032219 E018 13.9379741 0.0013082049 4.788023e-02 1.054127e-01 14 58329528 58329604 77 + 1.070 0.841 -0.860
ENSG00000032219 E019 17.7804465 0.0011991085 5.940111e-02 1.256096e-01 14 58330003 58330169 167 + 1.162 0.976 -0.674
ENSG00000032219 E020 0.2903454 0.2648898819 1.000000e+00   14 58331287 58331518 232 + 0.097 0.000 -9.595
ENSG00000032219 E021 11.5520774 0.0130543731 2.223600e-01 3.532186e-01 14 58344695 58344767 73 + 0.984 0.841 -0.541
ENSG00000032219 E022 15.3217750 0.0012618052 5.935405e-04 2.510120e-03 14 58346411 58346505 95 + 1.122 0.642 -1.856
ENSG00000032219 E023 13.4975373 0.0015690729 2.562309e-02 6.317224e-02 14 58347020 58347117 98 + 1.061 0.784 -1.048
ENSG00000032219 E024 22.6745971 0.0008499167 2.042563e-01 3.314894e-01 14 58347647 58347878 232 + 1.249 1.163 -0.307
ENSG00000032219 E025 23.0689002 0.0016118193 7.578364e-01 8.425311e-01 14 58351073 58351296 224 + 1.240 1.254 0.048
ENSG00000032219 E026 11.3796339 0.0253026414 6.352974e-01 7.496324e-01 14 58351297 58351323 27 + 0.967 0.935 -0.119
ENSG00000032219 E027 2.1874309 0.0093590747 9.849321e-01 9.946055e-01 14 58351324 58351676 353 + 0.398 0.431 0.175
ENSG00000032219 E028 0.8836079 0.0135329737 3.969339e-01 5.407083e-01 14 58353454 58353657 204 + 0.243 0.000 -11.545
ENSG00000032219 E029 29.2478195 0.0006484236 8.244703e-01 8.894184e-01 14 58353658 58353855 198 + 1.330 1.393 0.218
ENSG00000032219 E030 18.8153181 0.0025308610 2.311449e-01 3.638349e-01 14 58359132 58359216 85 + 1.130 1.293 0.576
ENSG00000032219 E031 20.6399372 0.0009413171 6.044388e-01 7.249934e-01 14 58360901 58361042 142 + 1.198 1.187 -0.036
ENSG00000032219 E032 78.3407569 0.0008600642 6.654650e-01 7.731436e-01 14 58364170 58365300 1131 + 1.742 1.810 0.232
ENSG00000032219 E033 0.7416549 0.0188973632 8.953767e-02 1.744179e-01 14 58365475 58365517 43 + 0.097 0.431 2.763
ENSG00000032219 E034 18.5638050 0.0066242719 1.369879e-02 3.753646e-02 14 58365518 58365622 105 + 1.088 1.379 1.028
ENSG00000032219 E035 11.9046459 0.0015002395 3.215664e-01 4.648220e-01 14 58366024 58366068 45 + 0.948 1.109 0.589
ENSG00000032219 E036 22.0135741 0.0009270650 3.644344e-01 5.088034e-01 14 58366069 58366230 162 + 1.201 1.329 0.449
ENSG00000032219 E037 19.3978811 0.0010403108 2.041909e-02 5.237105e-02 14 58366883 58367029 147 + 1.118 1.378 0.914
ENSG00000032219 E038 81.2261574 0.0250762546 2.572368e-06 1.954931e-05 14 58371886 58373887 2002 + 1.681 2.087 1.367